{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T06:38:14Z","timestamp":1771569494150,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,12,1]]},"abstract":"<jats:p>The hydrolysis-resistant GTP analogue GTP-gamma-S was introduced into rods isolated from the retina of the salamander Ambystoma tigrinum to study the origin of the persistent excitation induced by intense bleaching illumination. Dialysis of a dark-adapted rod with a whole-cell patch pipette containing 2 mM GTP-gamma-S resulted in a gradual decrease in circulating current. If the rod was first bleached and its sensitivity allowed to stabilize for at least 30 min, then dialysis with GTP-gamma-S produced a much faster current decay. The circulating current could be restored by superfusion with the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, suggesting that the decay in current originated from persistent excitation of the phosphodiesterase by transducin bound to GTP-gamma-S. We conclude that the persistent excitation which follows bleaching is likely to involve the GTP-binding protein transducin, which mediates the normal photoresponse. This observation suggests that a form of rhodopsin which persists long after bleaching can activate transducin much as does photoisomerized rhodopsin, although with considerably lower gain.<\/jats:p>","DOI":"10.1085\/jgp.108.6.557","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T23:51:34Z","timestamp":1084492294000},"page":"557-563","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":23,"title":["Persistent activation of transducin by bleached rhodopsin in salamander rods."],"prefix":"10.1085","volume":"108","author":[{"given":"H R","family":"Matthews","sequence":"first","affiliation":[{"name":"Physiological Laboratory, University of Cambridge, United Kingdom. hrml@cam.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M C","family":"Cornwall","sequence":"additional","affiliation":[{"name":"Physiological Laboratory, University of Cambridge, United Kingdom. hrml@cam.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G L","family":"Fain","sequence":"additional","affiliation":[{"name":"Physiological Laboratory, University of Cambridge, United Kingdom. hrml@cam.ac.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1996,12,1]]},"container-title":["The Journal of general physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/108\/6\/557\/1728523\/557.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/108\/6\/557\/1728523\/557.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T20:17:27Z","timestamp":1690316247000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/108\/6\/557\/26534\/Persistent-activation-of-transducin-by-bleached"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,12,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.108.6.557","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12,1]]}}}