{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:47:47Z","timestamp":1762112867049},"reference-count":41,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,3,1]]},"abstract":"<jats:p>The Shaker B K+ conductance (GK) collapses when the channels are closed (deactivated) in Na+ solutions that lack K+ ions. Also, it is known that external TEA (TEAo) impedes the collapse of GK (Go\u0301mez-Lagunas, F. 1997. J. Physiol. 499:3\u201315; Go\u0301mez-Lagunas, F. 2001. J. Gen. Physiol. 118:639\u2013648), and that channel block by TEAo and scorpion toxins are two mutually exclusive events (Goldstein, S.A.N., and C. Miller. 1993. Biophys. J. 65:1613\u20131619). Therefore, we tested the ability of scorpion toxins to inhibit the collapse of GK in 0 K+. We have found that these toxins are not uniform regarding the capacity to protect GK. Those toxins, whose binding to the channels is destabilized by external K+, are also effective inhibitors of the collapse of GK. In addition to K+, other externally added cations also destabilize toxin block, with an effectiveness that does not match the selectivity sequence of K+ channels. The inhibition of the drop of GK follows a saturation relationship with [toxin], which is fitted well by the Michaelis-Menten equation, with an apparent Kd bigger than that of block of the K+ current. However, another plausible model is also presented and compared with the Michaelis-Menten model. The observations suggest that those toxins that protect GK in 0 K+ do so by interacting either with the most external K+ binding site of the selectivity filter (suggesting that the K+ occupancy of only that site of the pore may be enough to preserve GK) or with sites capable of binding K+ located in the outer vestibule of the pore, above the selectivity filter.<\/jats:p>","DOI":"10.1085\/jgp.200308871","type":"journal-article","created":{"date-parts":[[2004,2,23]],"date-time":"2004-02-23T19:43:07Z","timestamp":1077565387000},"page":"265-279","update-policy":"http:\/\/dx.doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Inhibition of the Collapse of the <i>Shaker<\/i> K+ Conductance by Specific Scorpion Toxins"],"prefix":"10.1085","volume":"123","author":[{"given":"Froylan","family":"Go\u0301mez-Lagunas","sequence":"first","affiliation":[{"name":"1Department of Physiology, School of Medicine, National Autonomous University of Me\u0301xico, Ciudad Universitaria, Me\u0301xico City D.F. 04510, Me\u0301xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar V.F.","family":"Batista","sequence":"additional","affiliation":[{"name":"2Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Me\u0301xico, Cuernavaca 62210, Me\u0301xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timoteo","family":"Olamendi-Portugal","sequence":"additional","affiliation":[{"name":"2Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Me\u0301xico, Cuernavaca 62210, Me\u0301xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha E.","family":"Rami\u0301rez-Domi\u0301nguez","sequence":"additional","affiliation":[{"name":"2Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Me\u0301xico, Cuernavaca 62210, Me\u0301xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[{"name":"2Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Me\u0301xico, Cuernavaca 62210, Me\u0301xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2004,2,23]]},"reference":[{"key":"2023072919500141700_BIB1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/0896-6273(95)90104-3","volume":"15","year":"1995","journal-title":"Neuron."},{"key":"2023072919500141700_BIB2","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1085\/jgp.91.3.317","volume":"91","year":"1988","journal-title":"J. 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