{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:11:56Z","timestamp":1791277916597,"version":"4.1.0"},"reference-count":63,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,2,1]]},"abstract":"<jats:p>Using whole-cell recording in Drosophila S2 cells, we characterized a Ca2+-selective current that is activated by depletion of intracellular Ca2+ stores. Passive store depletion with a Ca2+-free pipette solution containing 12 mM BAPTA activated an inwardly rectifying Ca2+ current with a reversal potential &amp;gt;60 mV. Inward currents developed with a delay and reached a maximum of 20\u201350 pA at \u2212110 mV. This current doubled in amplitude upon increasing external Ca2+ from 2 to 20 mM and was not affected by substitution of choline for Na+. A pipette solution containing \u223c300 nM free Ca2+ and 10 mM EGTA prevented spontaneous activation, but Ca2+ current activated promptly upon application of ionomycin or thapsigargin, or during dialysis with IP3. Isotonic substitution of 20 mM Ca2+ by test divalent cations revealed a selectivity sequence of Ba2+ &amp;gt; Sr2+ &amp;gt; Ca2+ &amp;gt;&amp;gt; Mg2+. Ba2+ and Sr2+ currents inactivated within seconds of exposure to zero-Ca2+ solution at a holding potential of 10 mV. Inactivation of Ba2+ and Sr2+ currents showed recovery during strong hyperpolarizing pulses. Noise analysis provided an estimate of unitary conductance values in 20 mM Ca2+ and Ba2+ of 36 and 420 fS, respectively. Upon removal of all external divalent ions, a transient monovalent current exhibited strong selectivity for Na+ over Cs+. The Ca2+ current was completely and reversibly blocked by Gd3+, with an IC50 value of \u223c50 nM, and was also blocked by 20 \u03bcM SKF 96365 and by 20 \u03bcM 2-APB. At concentrations between 5 and 14 \u03bcM, application of 2-APB increased the magnitude of Ca2+ currents. We conclude that S2 cells express store-operated Ca2+ channels with many of the same biophysical characteristics as CRAC channels in mammalian cells.<\/jats:p>","DOI":"10.1085\/jgp.200308982","type":"journal-article","created":{"date-parts":[[2004,1,26]],"date-time":"2004-01-26T15:26:12Z","timestamp":1075130772000},"page":"167-182","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":76,"title":["A Store-operated Calcium Channel in\n                    <i>Drosophila<\/i>\n                    S2 Cells"],"prefix":"10.1085","volume":"123","author":[{"given":"Andriy V.","family":"Yeromin","sequence":"first","affiliation":[{"name":"1Department of Physiology and Biophysics, University of California, Irvine, CA 92697"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Roos","sequence":"additional","affiliation":[{"name":"2Neurogenetics, Inc., La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth A.","family":"Stauderman","sequence":"additional","affiliation":[{"name":"2Neurogenetics, Inc., La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Cahalan","sequence":"additional","affiliation":[{"name":"1Department of Physiology and Biophysics, University of California, Irvine, CA 92697"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2004,1,26]]},"reference":[{"key":"2023072617323995800_BIB1","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1113\/jphysiol.1984.sp015352","volume":"353","year":"1984","journal-title":"J. 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