{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T13:35:49Z","timestamp":1770298549028,"version":"3.49.0"},"reference-count":53,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:p>Recently, it was reported that the epithelial Na+ channel (ENaC) is regulated by temperature (Askwith, C.C., C.J. Benson, M.J. Welsh, and P.M. Snyder. 2001. Proc. Natl. Acad. Sci. USA. 98:6459\u20136463). As these changes of temperature affect membrane lipid order and lipid\u2013protein interactions, we tested the hypothesis that ENaC activity can be modulated by membrane lipid interactions. Two approaches were used to modulate membrane anisotropy, a lipid order\u2013dependent parameter. The nonpharmacological approach used temperature changes, while the pharmacological one used chlorpromazine (CPZ), an agent known to decrease membrane order, and Gd+3. Experiments used Xenopus oocytes expressing human ENaC. Methods of impedance analysis were used to determine whether the effects of changing lipid order indirectly altered ENaC conductance via changes of membrane area. These data were further corroborated with quantitative morphology on micrographs from oocytes membranes studied via electron microscopy. We report biphasic effects of cooling (stimulation followed by inhibition) on hENaC conductance. These effects were relatively slow (minutes) and were delayed from the actual bath temperature changes. Peak stimulation occurred at a calculated Tmax of 15.2. At temperatures below Tmax, ENaC conductance was inhibited with cooling. The effects of temperature on gNa were distinct from those observed on ion channels endogenous to Xenopus oocytes, where the membrane conductance decreased monoexponentially with temperature (t = 6.2\u00b0C). Similar effects were also observed in oocytes with reduced intra- and extracellular [Na+], thereby ruling out effects of self or feedback inhibition. Addition of CPZ or the mechanosensitive channel blocker, Gd+3, caused inhibition of ENaC. The effects of Gd+3 were also attributed to its ability to partition into the outer membrane leaflet and to decrease anisotropy. None of the effects of temperature, CPZ, or Gd+3 were accompanied by changes of membrane area, indicating the likely absence of effects on channel trafficking. However, CPZ and Gd+3 altered membrane capacitance in an opposite manner to temperature, consistent with effects on the membrane-dielectric properties. The reversible effects of both Gd+3 and CPZ could also be blocked by cooling and trapping these agents in the rigidified membrane, providing further evidence for their mechanism of action. Our findings demonstrate a novel regulatory mechanism of ENaC.<\/jats:p>","DOI":"10.1085\/jgp.200308983","type":"journal-article","created":{"date-parts":[[2004,5,18]],"date-time":"2004-05-18T00:53:49Z","timestamp":1084841629000},"page":"709-727","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":55,"title":["ENaC\u2013Membrane Interactions"],"prefix":"10.1085","volume":"123","author":[{"given":"Mouhamed S.","family":"Awayda","sequence":"first","affiliation":[{"name":"1Department of Physiology, Tulane University Health Sciences Center, New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weijian","family":"Shao","sequence":"additional","affiliation":[{"name":"1Department of Physiology, Tulane University Health Sciences Center, New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengli","family":"Guo","sequence":"additional","affiliation":[{"name":"2Department of Cell Biology and Physiology, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Zeidel","sequence":"additional","affiliation":[{"name":"3Renal Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren G.","family":"Hill","sequence":"additional","affiliation":[{"name":"3Renal Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2004,5,17]]},"reference":[{"key":"2023072617353457800_BIB1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s002490100145","volume":"30","year":"2001","journal-title":"Eur. 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