{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T13:35:35Z","timestamp":1762954535201},"reference-count":53,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:p>High conductance, calcium- and voltage-activated potassium (BK, MaxiK) channels are widely expressed in mammals. In some tissues, the biophysical properties of BK channels are highly affected by coexpression of regulatory (\u03b2) subunits. The most remarkable effects of \u03b21 and \u03b22 subunits are an increase of the calcium sensitivity and the slow down of channel kinetics. However, the detailed characteristics of channels formed by \u03b1 and \u03b21 or \u03b22 are dissimilar, the most remarkable difference being a reduction of the voltage sensitivity in the presence of \u03b21 but not \u03b22. Here we reveal the molecular regions in these \u03b2 subunits that determine their differential functional coupling with the pore-forming \u03b1-subunit. We made chimeric constructs between \u03b21 and \u03b22 subunits, and BK channels formed by \u03b1 and chimeric \u03b2 subunits were expressed in Xenopus laevis oocytes. The electrophysiological characteristics of the resulting channels were determined using the patch clamp technique. Chimeric exchange of the different regions of the \u03b21 and \u03b22 subunits demonstrates that the NH3 and COOH termini are the most relevant regions in defining the behavior of either subunit. This strongly suggests that the intracellular domains are crucial for the fine tuning of the effects of these \u03b2 subunits. Moreover, the intracellular domains of \u03b21 are responsible for the reduction of the BK channel voltage dependence. This agrees with previous studies that suggested the intracellular regions of the \u03b1-subunit to be the target of the modulation by the \u03b21-subunit.<\/jats:p>","DOI":"10.1085\/jgp.200509370","type":"journal-article","created":{"date-parts":[[2006,1,31]],"date-time":"2006-01-31T01:14:02Z","timestamp":1138670042000},"page":"191-204","update-policy":"http:\/\/dx.doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":50,"title":["Structural Determinants for Functional Coupling Between the \u03b2 and \u03b1 Subunits in the Ca2+-activated K+ (BK) Channel"],"prefix":"10.1085","volume":"127","author":[{"given":"Patricio","family":"Orio","sequence":"first","affiliation":[{"name":"1Department of Biophysics and Molecular Physiology, Centro de Estudios Cienti\u0301ficos, Valdivia 5110246, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolima","family":"Torres","sequence":"additional","affiliation":[{"name":"1Department of Biophysics and Molecular Physiology, Centro de Estudios Cienti\u0301ficos, Valdivia 5110246, Chile"},{"name":"2Universidad del Valle, Cali, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricio","family":"Rojas","sequence":"additional","affiliation":[{"name":"1Department of Biophysics and Molecular Physiology, Centro de Estudios Cienti\u0301ficos, Valdivia 5110246, Chile"},{"name":"3Facultad de Ciencias, Universidad de Chile, Santiago 7800024, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Carvacho","sequence":"additional","affiliation":[{"name":"1Department of Biophysics and Molecular Physiology, Centro de Estudios Cienti\u0301ficos, Valdivia 5110246, Chile"},{"name":"4Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria L.","family":"Garcia","sequence":"additional","affiliation":[{"name":"5Department of Ion Channels, Merck Research Laboratories, Rahway, NJ 07065"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligia","family":"Toro","sequence":"additional","affiliation":[{"name":"6Department of Anesthesiology University of California, Los Angeles, CA 90095"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel A.","family":"Valverde","sequence":"additional","affiliation":[{"name":"7Grup de Canalopaties, Unitat de Senyalitzacio\u0301 Celular, Universitat Pompeu Fabra, Barcelona 08003, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramon","family":"Latorre","sequence":"additional","affiliation":[{"name":"1Department of Biophysics and Molecular Physiology, Centro de Estudios Cienti\u0301ficos, Valdivia 5110246, Chile"},{"name":"3Facultad de Ciencias, Universidad de Chile, Santiago 7800024, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2006,1,30]]},"reference":[{"key":"2023072521054125500_BIB1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1085\/jgp.200509346","volume":"126","year":"2005","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1085\/jgp.20028627","volume":"120","year":"2002","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB3","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1085\/jgp.200409052","volume":"123","year":"2004","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB4","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0014-5793(00)01584-2","volume":"474","year":"2000","journal-title":"FEBS Lett."},{"key":"2023072521054125500_BIB5","doi-asserted-by":"crossref","first-page":"4776","DOI":"10.1073\/pnas.081072398","volume":"98","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072521054125500_BIB6","doi-asserted-by":"crossref","first-page":"6453","DOI":"10.1074\/jbc.275.9.6453","volume":"275","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB7","doi-asserted-by":"crossref","first-page":"11277","DOI":"10.1073\/pnas.201250598","volume":"98","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072521054125500_BIB8","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1085\/jgp.116.3.411","volume":"116","year":"2000","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB9","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1085\/jgp.110.3.257","volume":"110","year":"1997","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB10","doi-asserted-by":"crossref","first-page":"32430","DOI":"10.1074\/jbc.273.49.32430","volume":"273","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB11","doi-asserted-by":"crossref","first-page":"34594","DOI":"10.1074\/jbc.M104689200","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB12","doi-asserted-by":"crossref","first-page":"4543","DOI":"10.1523\/JNEUROSCI.16-15-04543.1996","volume":"16","year":"1996","journal-title":"J. Neurosci."},{"key":"2023072521054125500_BIB13","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1172\/JCI200420347","volume":"113","year":"2004","journal-title":"J. Clin. Invest."},{"key":"2023072521054125500_BIB14","doi-asserted-by":"crossref","first-page":"16289","DOI":"10.1074\/jbc.273.26.16289","volume":"273","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB15","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1085\/jgp.20028605","volume":"120","year":"2002","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB16","first-page":"528","volume":"8","year":"1990","journal-title":"Biotechniques."},{"key":"2023072521054125500_BIB17","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0896-6273(01)00236-7","volume":"29","year":"2001","journal-title":"Neuron."},{"key":"2023072521054125500_BIB18","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/417515a","volume":"417","year":"2002","journal-title":"Nature."},{"key":"2023072521054125500_BIB19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1006\/geno.1998.5627","volume":"55","year":"1999","journal-title":"Genomics."},{"key":"2023072521054125500_BIB20","doi-asserted-by":"crossref","first-page":"10014","DOI":"10.1074\/jbc.M107682200","volume":"277","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB21","doi-asserted-by":"crossref","first-page":"23336","DOI":"10.1016\/S0021-9258(17)31658-7","volume":"269","year":"1994","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB22","doi-asserted-by":"crossref","first-page":"17274","DOI":"10.1016\/S0021-9258(17)32551-6","volume":"269","year":"1994","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB23","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1085\/jgp.117.6.583","volume":"117","year":"2001","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB24","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1085\/jgp.20028696","volume":"120","year":"2002","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB25","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1113\/jphysiol.1991.sp018861","volume":"443","year":"1991","journal-title":"J. Physiol."},{"key":"2023072521054125500_BIB26","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/0896-6273(95)90321-6","volume":"14","year":"1995","journal-title":"Neuron."},{"key":"2023072521054125500_BIB27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.bb.07.060178.000245","volume":"7","year":"1978","journal-title":"Annu. Rev. Biophys. Bioeng."},{"key":"2023072521054125500_BIB28","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0014-5793(96)83884-1","volume":"385","year":"1996","journal-title":"FEBS Lett."},{"key":"2023072521054125500_BIB29","doi-asserted-by":"crossref","first-page":"14066","DOI":"10.1073\/pnas.94.25.14066","volume":"94","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072521054125500_BIB30","doi-asserted-by":"crossref","first-page":"5562","DOI":"10.1073\/pnas.100118597","volume":"97","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072521054125500_BIB31","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1085\/jgp.115.6.719","volume":"115","year":"2000","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB32","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.neuron.2004.05.001","volume":"42","year":"2004","journal-title":"Neuron."},{"key":"2023072521054125500_BIB33","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1085\/jgp.200409236","volume":"125","year":"2005","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB34","first-page":"156","volume":"17","year":"2002","journal-title":"News Physiol. Sci."},{"key":"2023072521054125500_BIB35","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1085\/jgp.20028692","volume":"120","year":"2002","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB36","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/S0006-3495(97)78168-2","volume":"73","year":"1997","journal-title":"Biophys. J."},{"key":"2023072521054125500_BIB37","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1038\/8077","volume":"2","year":"1999","journal-title":"Nat. Neurosci."},{"key":"2023072521054125500_BIB38","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1007\/BF00388308","volume":"426","year":"1994","journal-title":"Pflugers Arch."},{"key":"2023072521054125500_BIB39","year":"1995"},{"key":"2023072521054125500_BIB40","first-page":"112","volume":"13","year":"1998","journal-title":"News Physiol. Sci."},{"key":"2023072521054125500_BIB41","doi-asserted-by":"crossref","first-page":"18994","DOI":"10.1074\/jbc.M200410200","volume":"277","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB42","doi-asserted-by":"crossref","first-page":"23211","DOI":"10.1074\/jbc.M910187199","volume":"275","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2023072521054125500_BIB43","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1126\/science.285.5435.1929","volume":"285","year":"1999","journal-title":"Science."},{"key":"2023072521054125500_BIB44","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0959-4388(98)80056-1","volume":"8","year":"1998","journal-title":"Curr. Opin. Neurobiol."},{"key":"2023072521054125500_BIB45","first-page":"185","volume":"3","year":"1995","journal-title":"Receptors Channels."},{"key":"2023072521054125500_BIB46","doi-asserted-by":"crossref","first-page":"14922","DOI":"10.1073\/pnas.93.25.14922","volume":"93","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072521054125500_BIB47","doi-asserted-by":"crossref","first-page":"4137","DOI":"10.1073\/pnas.96.7.4137","volume":"96","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072521054125500_BIB48","doi-asserted-by":"crossref","first-page":"5255","DOI":"10.1523\/JNEUROSCI.19-13-05255.1999","volume":"19","year":"1999","journal-title":"J. Neurosci."},{"key":"2023072521054125500_BIB49","doi-asserted-by":"crossref","first-page":"4890","DOI":"10.1523\/JNEUROSCI.20-13-04890.2000","volume":"20","year":"2000","journal-title":"J. Neurosci."},{"key":"2023072521054125500_BIB50","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nature00956","volume":"418","year":"2002","journal-title":"Nature."},{"key":"2023072521054125500_BIB51","doi-asserted-by":"crossref","first-page":"5585","DOI":"10.1523\/JNEUROSCI.1296-04.2004","volume":"24","year":"2004","journal-title":"J. Neurosci."},{"key":"2023072521054125500_BIB52","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1085\/jgp.117.6.607","volume":"117","year":"2001","journal-title":"J. Gen. Physiol."},{"key":"2023072521054125500_BIB53","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/nsb932","volume":"10","year":"2003","journal-title":"Nat. Struct. Biol."}],"container-title":["The Journal of General Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/127\/2\/191\/1733322\/191.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/127\/2\/191\/1733322\/191.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T21:07:55Z","timestamp":1690319275000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/127\/2\/191\/53200\/Structural-Determinants-for-Functional-Coupling"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,30]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,2,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.200509370","relation":{},"ISSN":["1540-7748","0022-1295"],"issn-type":[{"value":"1540-7748","type":"electronic"},{"value":"0022-1295","type":"print"}],"subject":[],"published":{"date-parts":[[2006,1,30]]}}}