{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:04:03Z","timestamp":1791216243879,"version":"4.1.0"},"reference-count":56,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,8,1]]},"abstract":"<jats:p>We present here evidence for the enhancement, at rest, of an inositol 1,4,5-trisphosphate (IP3)\u2013mediated calcium signaling pathway in myotubes from dystrophin-deficient cell lines (SolC1(\u2212)) as compared to a cell line from the same origin but transfected with mini-dystrophin (SolD(+)). With confocal microscopy, the number of sites discharging calcium (release site density [RSD]) was quantified and found more elevated in SolC1(\u2212) than in SolD(+) myotubes. Variations of membrane potential had no significant effect on this difference, and higher resting [Ca2+]i in SolC1(\u2212) (Marchand, E., B. Constantin, H. Balghi, M.C. Claudepierre, A. Cantereau, C. Magaud, A. Mouzou, G. Raymond, S. Braun, and C. Cognard. 2004. Exp. Cell Res. 297:363\u2013379) cannot explain alone higher RSD. The exposure with SR Ca2+ channel inhibitors (ryanodine and 2-APB) and phospholipase C inhibitor (U73122) significantly reduced RSD in both cell types but with a stronger effect in dystrophin-deficient SolC1(\u2212) myotubes. Immunocytochemistry allowed us to localize ryanodine receptors (RyRs) as well as IP3 receptors (IP3Rs), IP3R-1 and IP3R-2 isoforms, indicating the presence of both RyRs-dependent and IP3-dependent release systems in both cells. We previously reported evidence for the enhancement, through a Gi protein, of the IP3-mediated calcium signaling pathway in SolC1(\u2212) as compared to SolD(+) myotubes during a high K+ stimulation (Balghi, H., S. Sebille, B. Constantin, S. Patri, V. Thoreau, L. Mondin, E. Mok, A. Kitzis, G. Raymond, and C. Cognard. 2006. J. Gen. Physiol. 127:171\u2013182). Here we show that, at rest, these regulation mechanisms are also involved in the modulation of calcium release activities. The enhancement of resting release activity may participate in the calcium overload observed in dystrophin-deficient myotubes, and our findings support the hypothesis of the regulatory role of mini-dystrophin on intracellular signaling.<\/jats:p>","DOI":"10.1085\/jgp.200609559","type":"journal-article","created":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T04:35:11Z","timestamp":1153197311000},"page":"219-230","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Mini-dystrophin Expression Down-regulates IP3-mediated Calcium Release Events in Resting Dystrophin-deficient Muscle Cells"],"prefix":"10.1085","volume":"128","author":[{"given":"Haouaria","family":"Balghi","sequence":"first","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phane","family":"Sebille","sequence":"additional","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludivine","family":"Mondin","sequence":"additional","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Cantereau","sequence":"additional","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Constantin","sequence":"additional","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Raymond","sequence":"additional","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Cognard","sequence":"additional","affiliation":[{"name":"Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite\u0301 de Poitiers, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"2023072920161442300_BIB1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1085\/jgp.20028671","volume":"121","year":"2003","journal-title":"J. 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