{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T20:28:02Z","timestamp":1781555282088,"version":"3.54.5"},"reference-count":46,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,2,1]]},"abstract":"<jats:p>Voltage-dependent potassium (Kv) channels play a pivotal role in the modulation of macrophage physiology. Macrophages are professional antigen-presenting cells and produce inflammatory and immunoactive substances that modulate the immune response. Blockage of Kv channels by specific antagonists decreases macrophage cytokine production and inhibits proliferation. Numerous pharmacological agents exert their effects on specific target cells by modifying the activity of their plasma membrane ion channels. Investigation of the mechanisms involved in the regulation of potassium ion conduction is, therefore, essential to the understanding of potassium channel functions in the immune response to infection and inflammation. Here, we demonstrate that the biophysical properties of voltage-dependent K+ currents are modified upon activation or immunosuppression in macrophages. This regulation is in accordance with changes in the molecular characteristics of the heterotetrameric Kv1.3\/Kv1.5 channels, which generate the main Kv in macrophages. An increase in K+ current amplitude in lipopolysaccharide-activated macrophages is characterized by a faster C-type inactivation, a greater percentage of cumulative inactivation, and a more effective margatoxin (MgTx) inhibition than control cells. These biophysical parameters are related to an increase in Kv1.3 subunits in the Kv1.3\/Kv1.5 hybrid channel. In contrast, dexamethasone decreased the C-type inactivation, the cumulative inactivation, and the sensitivity to MgTx concomitantly with a decrease in Kv1.3 expression. Neither of these treatments apparently altered the expression of Kv1.5. Our results demonstrate that the immunomodulation of macrophages triggers molecular and biophysical consequences in Kv1.3\/Kv1.5 hybrid channels by altering the subunit stoichiometry.<\/jats:p>","DOI":"10.1085\/jgp.200910334","type":"journal-article","created":{"date-parts":[[2010,1,25]],"date-time":"2010-01-25T17:38:31Z","timestamp":1264441111000},"page":"135-147","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":71,"title":["Immunomodulation of voltage-dependent K+ channels in macrophages: molecular and biophysical consequences"],"prefix":"10.1085","volume":"135","author":[{"given":"N\u00faria","family":"Villalonga","sequence":"first","affiliation":[{"name":"Molecular Physiology Laboratory, Departament de Bioqu\u00edmica i Biolog\u00eda Molecular, Institut de Biomedicina, Universitat de Barcelona, E-08028 Barcelona, Spain 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miren","family":"David","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas \u201cAlberto Sols,\u201d (CSIC-UAM), E-28029 Madrid, Spain 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Bielanska","sequence":"additional","affiliation":[{"name":"Molecular Physiology Laboratory, Departament de Bioqu\u00edmica i Biolog\u00eda Molecular, Institut de Biomedicina, Universitat de Barcelona, E-08028 Barcelona, Spain 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n","family":"Vicente","sequence":"additional","affiliation":[{"name":"Molecular Physiology Laboratory, Departament de Bioqu\u00edmica i Biolog\u00eda Molecular, Institut de Biomedicina, Universitat de Barcelona, E-08028 Barcelona, Spain 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00faria","family":"Comes","sequence":"additional","affiliation":[{"name":"Molecular Physiology Laboratory, Departament de Bioqu\u00edmica i Biolog\u00eda Molecular, Institut de Biomedicina, Universitat de Barcelona, E-08028 Barcelona, Spain 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biom\u00e9dicas \u201cAlberto Sols,\u201d (CSIC-UAM), E-28029 Madrid, Spain 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Felipe","sequence":"additional","affiliation":[{"name":"Molecular Physiology Laboratory, Departament de Bioqu\u00edmica i Biolog\u00eda Molecular, Institut de Biomedicina, Universitat de Barcelona, E-08028 Barcelona, Spain 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2010,1,25]]},"reference":[{"key":"2023072922430495600_bib1","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1097\/01.anes.0000282100.32923.5c","article-title":"Kvbeta1.3 reduces the degree of stereoselective bupivacaine block of Kv1.5 channels","volume":"107","author":"Arias","year":"2007","journal-title":"Anesthesiology."},{"key":"2023072922430495600_bib2","first-page":"287","article-title":"Glucocorticoid induced up-regulation of a pituitary K+ channel mRNA in vitro and in vivo","volume":"1","author":"Attardi","year":"1993","journal-title":"Receptors Channels."},{"key":"2023072922430495600_bib3","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.resp.2007.12.007","article-title":"Upregulation of AQP3 and AQP5 induced by dexamethasone and ambroxol in A549 cells","volume":"161","author":"Ben","year":"2008","journal-title":"Respir. 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