{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:55:22Z","timestamp":1792713322985,"version":"4.5.0"},"reference-count":57,"publisher":"Rockefeller University Press","issue":"2","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31471370"],"award-info":[{"award-number":["31471370"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:p>The epididymis establishes a congenial environment for sperm maturation and protection. Its fluid is acidic, and the calcium concentration is low and declines along the length of the epididymal tubule. However, our knowledge of ionic currents and mechanisms of calcium homeostasis in rat epididymal epithelial cells remains enigmatic. In this study, to better understand calcium regulation in the epididymis, we use the patch-clamp method to record from single rat cauda epididymal principal cells. We detect a constitutively active Ca2+ current with characteristics that match the epithelial calcium channel TRPV6. Electrophysiological and pharmacological data also reveal a constitutively active calcium-activated chloride conductance (CaCC). Removal of extracellular calcium attenuates not only the TRPV6-like conductance, but also the CaCC. Lanthanide block is time dependent such that the TRPV6-like component is inhibited first, followed by the CaCC. The putative CaCC blocker niflumic acid partially inhibits whole-cell currents, whereas La3+ almost abolishes whole-cell currents in principal cells. Membrane potential measurements reveal an interplay between La3+-sensitive ion channels and those that are sensitive to the specific TMEM16A inhibitor tannic acid. In vivo perfusion of the cauda epididymal tubule shows a substantial rate of Ca2+ reabsorption from the luminal side, which is dose-dependently suppressed by ruthenium red, a putative blocker of epithelial Ca2+ channels and CaCC. Finally, we discover messenger RNA for both TRPV6 and TMEM16A in the rat epididymis and show that their proteins colocalize in the apical membrane of principal cells. Collectively, these data provide evidence for a coupling mechanism between TRPV6 and TMEM16A in principal cells that may play an important role in the regulation of calcium homeostasis in the epididymis.<\/jats:p>","DOI":"10.1085\/jgp.201611626","type":"journal-article","created":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T18:22:40Z","timestamp":1470075760000},"page":"161-182","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["Coupling of TRPV6 and TMEM16A in epithelial principal cells of the rat epididymis"],"prefix":"10.1085","volume":"148","author":[{"given":"Da Yuan","family":"Gao","sequence":"first","affiliation":[{"name":"School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew C.T.","family":"Leung","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon C.L.","family":"Au","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick Y.D.","family":"Wong","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnie W.C.","family":"Shum","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2016,8,1]]},"reference":[{"key":"2023072623533356200_bib1","doi-asserted-by":"publisher","first-page":"50872","DOI":"10.1074\/jbc.M308800200","article-title":"Store-operated Ca2+ current and TRPV6 channels in lymph node prostate cancer cells","volume":"278","author":"B\u00f6dding","year":"2003","journal-title":"J. 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