{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:54:04Z","timestamp":1789538044183,"version":"build-2803163510"},"reference-count":40,"publisher":"Rockefeller University Press","issue":"10","license":[{"start":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T00:00:00Z","timestamp":1629331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC01362"],"award-info":[{"award-number":["R01 DC01362"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC015439"],"award-info":[{"award-number":["R01 DC015439"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC014685"],"award-info":[{"award-number":["R01 DC014685"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,10,4]]},"abstract":"<jats:p>Although mechanoelectrical transducer (MET) channels have been extensively studied, uncertainty persists about their molecular architecture and single-channel conductance. We made electrical measurements from mouse cochlear outer hair cells (OHCs) to reexamine the MET channel conductance comparing two different methods. Analysis of fluctuations in the macroscopic currents showed that the channel conductance in apical OHCs determined from nonstationary noise analysis was about half that of single-channel events recorded after tip link destruction. We hypothesized that this difference reflects a bandwidth limitation in the noise analysis, which we tested by simulations of stochastic fluctuations in modeled channels. Modeling indicated that the unitary conductance depended on the relative values of the channel activation time constant and the applied low-pass filter frequency. The modeling enabled the activation time constant of the channel to be estimated for the first time, yielding a value of only a few microseconds. We found that the channel conductance, assayed with both noise and recording of single-channel events, was reduced by a third in a new deafness mutant, Tmc1 p.D528N. Our results indicate that noise analysis is likely to underestimate MET channel amplitude, which is better characterized from recordings of single-channel events.<\/jats:p>","DOI":"10.1085\/jgp.202112959","type":"journal-article","created":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T19:48:24Z","timestamp":1629402504000},"update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["The speed of the hair cell mechanotransducer channel revealed by fluctuation analysis"],"prefix":"10.1085","volume":"153","author":[{"given":"Maryline","family":"Beurg","sequence":"first","affiliation":[{"name":"Department of Neuroscience, University of Wisconsin School of Medicine and Public Health, Madison, WI 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Hoon","family":"Nam","sequence":"additional","affiliation":[{"name":"Departments of Mechanical Engineering and Biomechanical Engineering, University of Rochester, Rochester, NY 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0754-7239","authenticated-orcid":true,"given":"Robert","family":"Fettiplace","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, University of Wisconsin School of Medicine and Public Health, Madison, WI 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2021,8,19]]},"reference":[{"key":"2023072922532618100_bib1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1152\/advan.00006.2002","article-title":"Counting channels: a tutorial guide on ion channel fluctuation analysis","volume":"26","author":"Alvarez","year":"2002","journal-title":"Adv. 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