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However, when studying miniature glutamatergic currents at cerebellar synapses between parallel fibers and molecular layer interneurons, we found that these currents were sometimes organized in bursts of events occurring at high frequency (about 30 Hz). Bursts displayed homogeneous quantal size amplitudes. Furthermore, in the presence of the desensitization inhibitor cyclothiazide, successive events within a burst displayed quantal amplitude occlusion. Based on these findings, we conclude that bursts originate in individual synapses. Bursts were enhanced by increasing either the external potassium concentration or the external calcium concentration, and they were strongly inhibited when blocking voltage-gated calcium channels by cadmium. Bursts were prevalent in elevated potassium concentration during the formation of the molecular layer but were infrequent later in development. Since postsynaptic AMPA receptors are largely calcium permeant in developing parallel fiber-interneuron synapses, we propose that bursts involve presynaptic calcium transients implicating presynaptic voltage-gated calcium channels, together with postsynaptic calcium transients implicating postsynaptic AMPA receptors. These simultaneous pre- and postsynaptic calcium transients may contribute to the formation and\/or stabilization of synaptic connections.<\/jats:p>","DOI":"10.1085\/jgp.202213212","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T02:20:34Z","timestamp":1678760434000},"update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Depolarization-induced bursts of miniature synaptic currents in individual synapses of developing cerebellum"],"prefix":"10.1085","volume":"155","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3618-4791","authenticated-orcid":true,"given":"Bastien","family":"Le Guellec","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05f82e368","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Paris Cit\u00e9 1 , , Paris, France"},{"name":"Saints P\u00e8res Paris Institute for the Neurosciences, CNRS 1 , , Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7890-1392","authenticated-orcid":true,"given":"Laura C.","family":"Gomez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05f82e368","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Paris Cit\u00e9 1 , , Paris, France"},{"name":"Saints P\u00e8res Paris Institute for the Neurosciences, CNRS 1 , , Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1181-0116","authenticated-orcid":true,"given":"Gerardo","family":"Malagon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05f82e368","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Paris Cit\u00e9 1 , , Paris, France"},{"name":"Saints P\u00e8res Paris Institute for the Neurosciences, CNRS 1 , , Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3233-4860","authenticated-orcid":true,"given":"Thibault","family":"Collin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05f82e368","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Paris Cit\u00e9 1 , , Paris, France"},{"name":"Saints P\u00e8res Paris Institute for the Neurosciences, CNRS 1 , , Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6478-6880","authenticated-orcid":true,"given":"Alain","family":"Marty","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05f82e368","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 Paris Cit\u00e9 1 , , Paris, France"},{"name":"Saints P\u00e8res Paris Institute for the Neurosciences, CNRS 1 , , Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2023,4,3]]},"reference":[{"key":"2023040313302521400_","doi-asserted-by":"publisher","first-page":"6336","DOI":"10.1523\/JNEUROSCI.22-15-06336.2002","article-title":"Multimodal quantal release at individual hippocampal synapses: Evidence for no lateral inhibition","volume":"22","author":"Abenavoli","year":"2002","journal-title":"J. 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