{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:12:58Z","timestamp":1785406378242,"version":"3.56.0"},"reference-count":22,"publisher":"Rockefeller University Press","issue":"6","license":[{"start":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T00:00:00Z","timestamp":1680652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.rupress.org\/terms\/"},{"start":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T00:00:00Z","timestamp":1701561600000},"content-version":"vor","delay-in-days":242,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"EU Horizon 2020","doi-asserted-by":"publisher","award":["739593"],"award-info":[{"award-number":["739593"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["CSANAD21G0"],"award-info":[{"award-number":["CSANAD21G0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["144199"],"award-info":[{"award-number":["144199"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,6,5]]},"abstract":"<jats:p>Light-controlled availability for phosphorylation reveals dominant roles of select R-domain serines in CFTR channel activation.<\/jats:p>","DOI":"10.1085\/jgp.202313336","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T02:20:27Z","timestamp":1679451627000},"update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Blue flash sheds light on the roles of individual phosphoserines in CFTR channel activation"],"prefix":"10.1085","volume":"155","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-5889","authenticated-orcid":true,"given":"L\u00e1szl\u00f3","family":"Csan\u00e1dy","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01g9ty582","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Semmelweis University 1 , Budapest, Hungary"},{"name":"HCEMM-SE Molecular Channelopathies Research Group 2 , Budapest, Hungary"},{"name":"ELKH-SE Ion Channel Research Group 3 , Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2023,4,5]]},"reference":[{"key":"2023041813463691900_bib1","doi-asserted-by":"publisher","first-page":"11304","DOI":"10.1016\/S0021-9258(18)82125-1","article-title":"Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites","volume":"268","author":"Chang","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023041813463691900_bib2","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1016\/0092-8674(91)90446-6","article-title":"Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channel","volume":"66","author":"Cheng","year":"1991","journal-title":"Cell"},{"key":"2023041813463691900_bib3","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1085\/jgp.116.3.477","article-title":"Severed channels probe regulation of gating of cystic fibrosis transmembrane conductance regulator by its cytoplasmic domains","volume":"116","author":"Csan\u00e1dy","year":"2000","journal-title":"J. Gen. Physiol."},{"key":"2023041813463691900_bib4","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1085\/jgp.200409076","article-title":"Preferential phosphorylation of R-domain Serine 768 dampens activation of CFTR channels by PKA","volume":"125","author":"Csan\u00e1dy","year":"2005","journal-title":"J. Gen. Physiol."},{"key":"2023041813463691900_bib5","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.devcel.2019.09.017","article-title":"An ancient CFTR ortholog informs molecular evolution in ABC transporters","volume":"51","author":"Cui","year":"2019","journal-title":"Dev. Cell"},{"key":"2023041813463691900_bib6","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1021\/acs.accounts.0c00292","article-title":"Useful caged compounds for cell physiology","volume":"53","author":"Ellis-Davies","year":"2020","journal-title":"Acc. Chem. Res."},{"key":"2023041813463691900_bib7","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.1016\/j.bbamem.2009.03.015","article-title":"Role of individual R domain phosphorylation sites in CFTR regulation by protein kinase A","volume":"1788","author":"Hegedus","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"2023041813463691900_bib8","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202213216","article-title":"Real-time observation of functional specialization among phosphorylation sites in CFTR","volume":"155","author":"Infield","year":"2023","journal-title":"J. Gen. Physiol."},{"key":"2023041813463691900_bib9","doi-asserted-by":"publisher","first-page":"1929","DOI":"10.1021\/bi00603a020","article-title":"Rapid photolytic release of adenosine 5\u2032-triphosphate from a protected analogue: Utilization by the Na:K pump of human red blood cell ghosts","volume":"17","author":"Kaplan","year":"1978","journal-title":"Biochemistry"},{"key":"2023041813463691900_bib10","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.cell.2017.02.024","article-title":"Molecular structure of the human CFTR ion channel","volume":"169","author":"Liu","year":"2017","journal-title":"Cell"},{"key":"2023041813463691900_bib11","doi-asserted-by":"publisher","first-page":"21740","DOI":"10.1073\/pnas.2007910117","article-title":"Simple binding of protein kinase A prior to phosphorylation allows CFTR anion channels to be opened by nucleotides","volume":"117","author":"Mih\u00e1lyi","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023041813463691900_bib12","doi-asserted-by":"publisher","first-page":"12742","DOI":"10.1016\/S0021-9258(18)42339-3","article-title":"Phosphorylation of the cystic fibrosis transmembrane conductance regulator","volume":"267","author":"Picciotto","year":"1992","journal-title":"J. Biol. Chem."},{"key":"2023041813463691900_bib13","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1146\/annurev-pharmtox-011112-140343","article-title":"Unnatural amino acids as probes of ligand-receptor interactions and their conformational consequences","volume":"53","author":"Pless","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"2023041813463691900_bib14","doi-asserted-by":"publisher","first-page":"20259","DOI":"10.1016\/S0021-9258(20)80723-6","article-title":"Regulation of the cystic fibrosis transmembrane conductance regulator Cl\u2212 channel by negative charge in the R domain","volume":"268","author":"Rich","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2023041813463691900_bib15","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1126\/science.1712985","article-title":"Effect of deleting the R domain on CFTR-generated chloride channels","volume":"253","author":"Rich","year":"1991","journal-title":"Science"},{"key":"2023041813463691900_bib16","doi-asserted-by":"publisher","first-page":"2158","DOI":"10.1074\/jbc.270.5.2158","article-title":"cAMP-dependent protein kinase-mediated phosphorylation of cystic fibrosis transmembrane conductance regulator residue Ser-753 and its role in channel activation","volume":"270","author":"Seibert","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2023041813463691900_bib17","doi-asserted-by":"publisher","first-page":"1865","DOI":"10.1002\/pro.5560050912","article-title":"Identification of protein kinase A phosphorylation sites on NBD1 and R domains of CFTR using electrospray mass spectrometry with selective phosphate ion monitoring","volume":"5","author":"Townsend","year":"1996","journal-title":"Protein Sci."},{"key":"2023041813463691900_bib18","doi-asserted-by":"publisher","first-page":"C737","DOI":"10.1152\/ajpcell.00504.2003","article-title":"Dibasic phosphorylation sites in the R domain of CFTR have stimulatory and inhibitory effects on channel activation","volume":"287","author":"Vais","year":"2004","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2023041813463691900_bib19","doi-asserted-by":"publisher","first-page":"876","DOI":"10.1038\/nature03313","article-title":"CFTR channel opening by ATP-driven tight dimerization of its nucleotide-binding domains","volume":"433","author":"Vergani","year":"2005","journal-title":"Nature"},{"key":"2023041813463691900_bib20","doi-asserted-by":"publisher","first-page":"L127","DOI":"10.1152\/ajplung.1997.273.1.L127","article-title":"CFTR activation: Additive effects of stimulatory and inhibitory phosphorylation sites in the R domain","volume":"273","author":"Wilkinson","year":"1997","journal-title":"Am. J. Physiol."},{"key":"2023041813463691900_bib21","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1038\/38514","article-title":"Stimulation of CFTR activity by its phosphorylated R domain","volume":"389","author":"Winter","year":"1997","journal-title":"Nature"},{"key":"2023041813463691900_bib22","doi-asserted-by":"publisher","first-page":"12757","DOI":"10.1073\/pnas.1815287115","article-title":"Molecular structure of the ATP-bound, phosphorylated human CFTR","volume":"115","author":"Zhang","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Journal of General Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/doi\/10.1085\/jgp.202313336\/1451264\/jgp_202313336.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/doi\/10.1085\/jgp.202313336\/1451264\/jgp_202313336.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T09:46:57Z","timestamp":1681811217000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/155\/6\/e202313336\/213998\/Blue-flash-sheds-light-on-the-roles-of-individual"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,5]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,5]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.202313336","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,5]]},"article-number":"e202313336"}}