{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:29:48Z","timestamp":1787243388325,"version":"build-2736575974"},"reference-count":18,"publisher":"Rockefeller University Press","issue":"1","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/rupress.org\/pages\/terms102024"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NHLBI R00141698"],"award-info":[{"award-number":["NHLBI R00141698"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R56165137"],"award-info":[{"award-number":["R56165137"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["23POST1023125"],"award-info":[{"award-number":["23POST1023125"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,1,5]]},"abstract":"<jats:p>Heart function depends on cardiomyocyte contractile apparatus and proper sarcomere protein expression. Variants in sarcomere genes cause inherited forms of cardiomyopathy and arrhythmias, including atrial fibrillation. Recently, a sarcomere component, myosin-binding protein-H like (MyBP-HL), was identified. MyBP-HL is mainly expressed in cardiac atria and is homologous to the last three C-terminal domains of cardiac myosin-binding protein-C (cMyBP-C). The MYBPHL R255X nonsense variant has been linked to atrial enlargement, dilated cardiomyopathy, and arrhythmias. Similar nonsense mutations in MYBPC3 are linked to hypertrophic cardiomyopathy, with these mutations preventing myofilament incorporation and the degradation of the truncated protein. However, the allele frequency of the MYBPHL R255X variant is too high in the human population to be pathogenic. We sought to determine whether MYBPHL nonsense variants impact on MyBP-HL sarcomere integration and degradation of the truncated protein, and whether the MyBPHL nonsense variants lead to changes in cardiomyocyte calcium dynamics and contractility. We mimicked human MYBPHL nonsense variants in the mouse Mybphl cDNA sequence and tested their sarcomere incorporation. We demonstrated that full-length MyBP-HL overexpression showed the expected C-zone sarcomere incorporation. Nonsense variants showed defective sarcomere incorporation. We demonstrated that full-length MyBP-HL and MyBP-HL nonsense variants were degraded by both proteasome and calpain mechanisms. We did not observe changes in calcium transients. In addition, we observed changes in contraction kinetics, including sarcomere shortening. Together, these data support the hypothesis that MYBPHL nonsense variants are functionally similar.<\/jats:p>","DOI":"10.1085\/jgp.202413630","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T06:20:30Z","timestamp":1758608430000},"update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Myosin-binding protein H-like nonsense variants exhibit impaired sarcomere incorporation and alter contractility"],"prefix":"10.1085","volume":"158","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9608-3004","authenticated-orcid":true,"given":"Alejandro","family":"Alvarez-Arce","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0260-6384","authenticated-orcid":true,"given":"Geena E.","family":"Fritzmann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9912-019X","authenticated-orcid":true,"given":"Hope V.","family":"Burnham","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1813-3621","authenticated-orcid":true,"given":"Kelly N.","family":"Araujo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3356-6345","authenticated-orcid":true,"given":"Alexandra","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6574-8533","authenticated-orcid":true,"given":"Lucas M.","family":"Wittenkeller","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7760-1143","authenticated-orcid":true,"given":"Hannah E.","family":"Cizauskas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6166-640X","authenticated-orcid":true,"given":"David Y.","family":"Barefield","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6x2g63","id-type":"ROR","asserted-by":"publisher"}],"name":"Loyola University Chicago 1 Department of Cell and Molecular Physiology, , Maywood, IL,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2025,11,19]]},"reference":[{"key":"2025111909464697000_bib2","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1016\/j.yjmcc.2009.11.014","article-title":"Phosphorylation and function of cardiac myosin binding protein-C in health and disease","volume":"48","author":"Barefield","year":"2010","journal-title":"J. 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