{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T07:38:35Z","timestamp":1768549115469,"version":"3.49.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1948,11,20]]},"abstract":"<jats:p>Crystalline soybean trypsin inhibitor protein undergoes denaturation on heating which is reversed on cooling. In the range of temperature of 35 to 50\u00b0C. a solution of the protein consists of a mixture of native and denatured forms in equilibrium with each other. The equilibrium is only slowly established and its final value at any temperature is the same whether a heated, denatured solution of the protein is cooled to the given temperature or whether a fresh solution is raised to that temperature. The kinetics of reversible denaturation of the soybean protein as well as the reversal of denaturation is that of a reversible unimolecular reaction, each process consisting at a given temperature of the same two simultaneous reactions acting in opposite directions.<\/jats:p>\n               <jats:p>The experimental data on the effect of temperature on the velocity and the equilibrium constants of the opposing reaction were utilized in evaluating the reaction energies and activation energies.<\/jats:p>\n               <jats:p>The reaction energies for denaturation were found to be as follows:\u2014<\/jats:p>\n               <jats:p>Change in total heat of reaction \u0394H = 57,000 calories per mole<\/jats:p>\n               <jats:p>Change in entropy of reaction \u0394S = 180 calories per degree per mole<\/jats:p>\n               <jats:p>The heat of activation \u0394H1\u2021 for denaturation = 55,000<\/jats:p>\n               <jats:p>The heat of activation \u0394H2\u2021 for the reversal of denaturation = \u20131900<\/jats:p>\n               <jats:p>The entropy \u0394S1\u2021 for denaturation = 95<\/jats:p>\n               <jats:p>The entropy \u0394S2\u2021 for reversal of denaturation = \u201384<\/jats:p>","DOI":"10.1085\/jgp.32.2.241","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T21:41:10Z","timestamp":1084484470000},"page":"241-263","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":73,"title":["THE KINETICS AND THERMODYNAMICS OF REVERSIBLE DENATURATION OF CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR"],"prefix":"10.1085","volume":"32","author":[{"given":"M.","family":"Kunitz","sequence":"first","affiliation":[{"name":"From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1948,11,20]]},"container-title":["Journal of General Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/rupress.org\/jgp\/article-pdf\/32\/2\/241\/1240331\/241.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/rupress.org\/jgp\/article-pdf\/32\/2\/241\/1240331\/241.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T19:14:16Z","timestamp":1601752456000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/32\/2\/241\/12065\/THE-KINETICS-AND-THERMODYNAMICS-OF-REVERSIBLE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1948,11,20]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1948,11,20]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.32.2.241","relation":{},"ISSN":["1540-7748","0022-1295"],"issn-type":[{"value":"1540-7748","type":"electronic"},{"value":"0022-1295","type":"print"}],"subject":[],"published":{"date-parts":[[1948,11,20]]}}}