{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T11:46:08Z","timestamp":1759146368727},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,1,1]]},"abstract":"<jats:p>Properties of the whole-cell K+ currents and voltage-dependent activation and inactivation properties of single K+ channels in clonal pheochromocytoma (PC-12) cells were studied using the patch-clamp recording technique. Depolarizing pulses elicited slowly inactivating whole-cell K+ currents, which were blocked by external application of tetraethylammonium+, 4-aminopyridine, and quinidine. The amplitudes and time courses of these K+ currents were largely independent of the prepulse voltage. Although pharmacological agents and manipulation of the voltage-clamp pulse protocol failed to reveal any additional separable whole-cell currents in a majority of the cells examined, single-channel recordings showed that, in addition to the large Ca++-dependent K+ channels described previously in many other preparations, PC-12 cells had at least four distinct types of K+ channels activated by depolarization. These four types of K+ channels differed in the open-channel current-voltage relation, time course of activation and inactivation, and voltage dependence of activation and inactivation. These K+ channels were designated the Kw, Kz, Ky, and Kx channels. The typical chord conductances of these channels were 18, 12, 7, and 7 pS in the excised configuration using Na+-free saline solutions. These four types of K+ channels opened in the presence of low concentrations of internal Ca++ (1 nM). Their voltage-dependent gating properties can account for the properties of the whole-cell K+ currents in PC-12 cells.<\/jats:p>","DOI":"10.1085\/jgp.91.1.73","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T23:48:59Z","timestamp":1084492139000},"page":"73-106","update-policy":"http:\/\/dx.doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["Voltage-dependent K+ currents and underlying single K+ channels in pheochromocytoma cells."],"prefix":"10.1085","volume":"91","author":[{"given":"T","family":"Hoshi","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Stanford University School of Medicine, California 94305."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R W","family":"Aldrich","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Stanford University School of Medicine, California 94305."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1988,1,1]]},"container-title":["The Journal of general physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/91\/1\/73\/1813582\/73.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/91\/1\/73\/1813582\/73.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T12:25:11Z","timestamp":1690460711000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/91\/1\/73\/27629\/Voltage-dependent-K-currents-and-underlying-single"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,1,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.91.1.73","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,1,1]]}}}