{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:10:39Z","timestamp":1758280239627},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,5,1]]},"abstract":"<jats:p>We used patch clamp techniques to identify and characterize a variety of K+ channels in primary human peripheral T lymphocytes. The most common channel observed in cell-attached configuration was voltage gated and inactivating. In ensemble averages, the kinetics of its activation and inactivation were similar to those of the whole-cell, voltage-gated K+ current described previously (Cahalan, M. D., K. G. Chandy, T. E. DeCoursey, and S. Gupta. 1985. J. Physiol. [Lond.]. 358:197-237; Deutsch, C., D. Krause, and S. C. Lee. 1986. J. Physiol. [Lond.]. 372:405-423), suggesting that this channel underlies the major portion of the outward current in lymphocytes. A small fraction of the time, this or another very similar channel was observed to inactivate significantly more slowly. Another channel type observed in cell-attached recording was seen less frequently and was transient in its appearance. This channel has a unitary conductance of approximately 10 pS, similar to the voltage-gated channel, but its voltage-independent gating, lack of inactivation, and different kinetic parameters showed it to be distinct. In whole-cell recording there is often a significant plateau current during sustained depolarization. Experiments using whole-cell and excised outside-out configurations indicate that at least part of this residual current is carried by K+ and, as opposed to the predominant voltage-gated current, is charybdotoxin insensitive. These findings are consistent with evidence that implicates charybdotoxin-sensitive and -insensitive components in T lymphocyte proliferation and volume regulation.<\/jats:p>","DOI":"10.1085\/jgp.99.5.771","type":"journal-article","created":{"date-parts":[[2004,5,13]],"date-time":"2004-05-13T23:48:59Z","timestamp":1084492139000},"page":"771-793","update-policy":"http:\/\/dx.doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":26,"title":["Diverse K+ channels in primary human T lymphocytes."],"prefix":"10.1085","volume":"99","author":[{"given":"S C","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D I","family":"Levy","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Deutsch","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1992,5,1]]},"container-title":["The Journal of general physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/99\/5\/771\/1814820\/771.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jgp\/article-pdf\/99\/5\/771\/1814820\/771.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T12:38:30Z","timestamp":1690461510000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jgp\/article\/99\/5\/771\/28036\/Diverse-K-channels-in-primary-human-T-lymphocytes"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,5,1]]}},"URL":"https:\/\/doi.org\/10.1085\/jgp.99.5.771","relation":{},"ISSN":["0022-1295","1540-7748"],"issn-type":[{"value":"0022-1295","type":"print"},{"value":"1540-7748","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5,1]]}}}