{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T01:49:25Z","timestamp":1775094565828,"version":"3.50.1"},"reference-count":33,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"<jats:p>A central step in the gating of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel is the association of its two cytosolic nucleotide-binding domains (NBDs) into a head-to-tail dimer, with two nucleotides bound at the interface. Channel opening and closing, respectively, are coupled to formation and disruption of this tight NBD dimer. CFTR is an asymmetric adenosine triphosphate (ATP)-binding cassette protein in which the two interfacial-binding sites (composite sites 1 and 2) are functionally different. During gating, the canonical, catalytically active nucleotide-binding site (site 2) cycles between dimerized prehydrolytic (state O1), dimerized post-hydrolytic (state O2), and dissociated (state C) forms in a preferential C\u2192O1\u2192O2\u2192C sequence. In contrast, the catalytically inactive nucleotide-binding site (site 1) is believed to remain associated, ATP-bound, for several gating cycles. Here, we have examined the possibility of conformational changes in site 1 during gating, by studying gating effects of perturbations in site 1.<\/jats:p>\n               <jats:p>Previous work showed that channel closure is slowed, both under hydrolytic and nonhydrolytic conditions, by occupancy of site 1 by N6-(2-phenylethyl)-ATP (P-ATP) as well as by the site-1 mutation H1348A (NBD2 signature sequence). Here, we found that P-ATP prolongs wild-type (WT) CFTR burst durations by selectively slowing (&amp;gt;2\u00d7) transition O1\u2192O2 and decreases the nonhydrolytic closing rate (transition O1\u2192C) of CFTR mutants K1250A (\u223c4\u00d7) and E1371S (\u223c3\u00d7). Mutation H1348A also slowed (\u223c3\u00d7) the O1\u2192O2 transition in the WT background and decreased the nonhydrolytic closing rate of both K1250A (\u223c3\u00d7) and E1371S (\u223c3\u00d7) background mutants. Neither P-ATP nor the H1348A mutation affected the 1:1 stoichiometry between ATP occlusion and channel burst events characteristic to WT CFTR gating in ATP. The marked effect that different structural perturbations at site 1 have on both steps O1\u2192C and O1\u2192O2 suggests that the overall conformational changes that CFTR undergoes upon opening and coincident with hydrolysis at the active site 2 include significant structural rearrangement at site 1.<\/jats:p>","DOI":"10.1085\/jgp.201210954","type":"journal-article","created":{"date-parts":[[2013,6,11]],"date-time":"2013-06-11T02:43:09Z","timestamp":1370918589000},"page":"61-73","update-policy":"https:\/\/doi.org\/10.1085\/jgp.crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["Conformational changes in the catalytically inactive nucleotide-binding site of CFTR"],"prefix":"10.1085","volume":"142","author":[{"given":"L\u00e1szl\u00f3","family":"Csan\u00e1dy","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry 1 and 2"},{"name":"MTA-SE Ion Channel Research Group, Semmelweis University, Budapest H-1094, Hungary 1 and 2"},{"name":"Department of Medical Biochemistry 1 and 2"},{"name":"MTA-SE Ion Channel Research Group, Semmelweis University, Budapest H-1094, Hungary 1 and 2"}]},{"given":"Csaba","family":"Mih\u00e1lyi","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry 1 and 2"},{"name":"MTA-SE Ion Channel Research Group, Semmelweis University, Budapest H-1094, Hungary 1 and 2"}]},{"given":"Andras","family":"Szollosi","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry 1 and 2"},{"name":"MTA-SE Ion Channel Research Group, Semmelweis University, Budapest H-1094, Hungary 1 and 2"}]},{"given":"Be\u00e1ta","family":"T\u00f6r\u00f6csik","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry 1 and 2"},{"name":"MTA-SE Ion Channel Research Group, Semmelweis University, Budapest H-1094, Hungary 1 and 2"}]},{"given":"Paola","family":"Vergani","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Physiology and Pharmacology, University College London, London WC1E 6BT, England, UK 3"}]}],"member":"291","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"2023072622485166200_bib1","doi-asserted-by":"publisher","first-page":"15419","DOI":"10.1074\/jbc.M111713200","article-title":"The first nucleotide binding domain of cystic fibrosis transmembrane conductance regulator is a site of stable nucleotide interaction, whereas the second is a site of rapid turnover","volume":"277","author":"Aleksandrov","year":"2002","journal-title":"J. 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