{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:34:45Z","timestamp":1790872485095,"version":"4.1.0"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2015,1,2]],"date-time":"2015-01-02T00:00:00Z","timestamp":1420156800000},"content-version":"unspecified","delay-in-days":4810,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infect. Control Hosp. Epidemiol."],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"S0195941700072623_abs1\">\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      To investigate the presence and clonal distribution of\n                      <jats:italic>Legionella<\/jats:italic>\n                      species in the water supply of 20 hospitals in Catalonia, Spain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S0195941700072623_abs2\">\n                    <jats:title>Setting:<\/jats:title>\n                    <jats:p>\n                      20 hospitals in Catalonia, an area of 32,000 km\n                      <jats:sup>2<\/jats:sup>\n                      , located in northeast Spain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S0195941700072623_abs3\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Environmental cultures of 186 points of potable water supply and 10 cooling towers were performed for the presence of\n                      <jats:italic>Legionella<\/jats:italic>\n                      species. Following filtration and acid treatment, the samples were seeded in selective MWY (modified Wadowsky Yee)-buffered charcoal yeast extract-a agar. All isolates obtained were characterized microbiologically and genotyped by\n                      <jats:italic>Sfil<\/jats:italic>\n                      pulsed-field gel electrophoresis (PFGE).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S0195941700072623_abs4\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      73 of 196 water samples, representing 17 of the 20 hospitals included in the study, were positive for\n                      <jats:italic>Legionella pneumophila<\/jats:italic>\n                      (serogroups 1, 2-14, or both). The degree of contamination ranged from 200 to 74,250 colony-forming units\/L. Twenty-five chromosomal DNA subtypes were detected by PFGE. A single DNA subtype was identified in 10 hospitals, 2 DNA subtypes were observed in 6 hospitals, and 1 hospital exhibited 3 different DNA subtypes. Each hospital had its own\n                      <jats:italic>Legionella<\/jats:italic>\n                      DNA subtype, which was not shared with any other hospitals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"S0195941700072623_abs5\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Legionella<\/jats:italic>\n                      was present in the water of most of the hospitals studied; each such hospital had a unique, dominant chromosomal DNA subtype. The verification of several genomic DNA restriction profiles in such a small geographic area demonstrates the great genetic diversity of\n                      <jats:italic>Legionella<\/jats:italic>\n                      in the aquatic environment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1017\/s0195941700072623","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T18:35:13Z","timestamp":1557254113000},"page":"673-676","source":"Crossref","is-referenced-by-count":112,"title":["Presence and Chromosomal Subtyping of\n                    <i>Legionella<\/i>\n                    Species in Potable Water Systems in 20 Hospitals of Catalonia, Spain"],"prefix":"10.1017","volume":"22","author":[{"given":"Miquel","family":"Sabri\u00e0","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Garc\u00eda-N\u00fa\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Pedro-Botet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Sopena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep 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