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Epidemiol."],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0899823X0019823X_abs1\"><jats:title>Objective:<\/jats:title><jats:p>To describe microbiological characteristics and epidemiologic features of an outbreak of postpartum endometritis.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X0019823X_abs2\"><jats:title>Methods:<\/jats:title><jats:p>Various markers were investigated in five patients and three throat carriage isolates of<jats:italic>Streptococcus pyogenes<\/jats:italic>obtained during an outbreak of endometritis occurring in a 13-week period. Molecular characterization included biotyping, T-serotyping,<jats:italic>emm<\/jats:italic>gene sequence and restriction, pulsed-field gel electrophoresis (PFGE), and random amplified polymorphic DNA (RAPD) analysis.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X0019823X_abs3\"><jats:title>Results:<\/jats:title><jats:p>Biotype, T-serotype, and genotypic data (<jats:italic>emm<\/jats:italic>analysis, PFGE, and RAPD analysis) revealed a close relationship among the isolates from three patients, suggesting that cross-contamination had occurred. These isolates were biotype 1, T type 28, and<jats:italic>emm<\/jats:italic>type 28. The isolates from one patient and one carrier differed from those of the index patient by minor variations of the<jats:italic>emm<\/jats:italic>amplicon restriction pattern, PFGE pattern, or RAPD pattern. The remaining isolates were phenotypically and genetically different.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X0019823X_abs4\"><jats:title>Conclusion:<\/jats:title><jats:p>Identification of different isolates demonstrated that different strains may circulate simultaneously during a true outbreak and that the predominant strain might persist for several months.<\/jats:p><\/jats:sec>","DOI":"10.1086\/502567","type":"journal-article","created":{"date-parts":[[2006,4,14]],"date-time":"2006-04-14T20:26:29Z","timestamp":1145046389000},"page":"455-461","source":"Crossref","is-referenced-by-count":26,"title":["Molecular Characterization of<i>Streptococcus Pyogenes<\/i>Isolates to investigate an outbreak of Puerperal 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