{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:37:44Z","timestamp":1791059864650,"version":"4.1.0"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2016,6,21]],"date-time":"2016-06-21T00:00:00Z","timestamp":1466467200000},"content-version":"unspecified","delay-in-days":4038,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infect. Control Hosp. Epidemiol."],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec id=\"S0899823X0019837X_abs1\"><jats:title>Background:<\/jats:title><jats:p>Central venous catheter (CVC)-related bloodstream infections (BSIs) are known to increase rates of morbidity and mortality in both inpatients and outpatients, including hematology-oncology patients and those undergoing hemodialysis or home infusion therapy. Biofilm-associated organisms on the lumens of these catheters have reduced susceptibility to antimicrobial chemotherapy. This study tested the efficacy of tetrasodium EDTA as a catheter lock solution on biofilms of several clinically relevant microorganisms.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X0019837X_abs2\"><jats:title>Methods:<\/jats:title><jats:p>Biofilms of<jats:italic>Staphylococcus epidermidis<\/jats:italic>, methicillin-resistant<jats:italic>S. aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae<\/jats:italic>, and<jats:italic>Candida albicans<\/jats:italic>were grown to levels of approximately 1 \u00d7 10<jats:sup>5<\/jats:sup>colony-forming units (CFU)\/cm<jats:sup>\u22121<\/jats:sup>on CVC segments in a model system, then subjected to the tetrasodium EDTA lock treatment.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X0019837X_abs3\"><jats:title>Results:<\/jats:title><jats:p>Comparisons of biofilms before and after exposure to the 40-mg\/mL<jats:sup>\u22121<\/jats:sup>tetrasodium EDTA lock for 21 hours showed that the biofilm viable cell counts of all organisms tested were significantly reduced (<jats:italic>P<\/jats:italic>&lt; .05) after exposure to the treatment.<\/jats:p><\/jats:sec><jats:sec id=\"S0899823X0019837X_abs4\"><jats:title>Conclusion:<\/jats:title><jats:p>Antimicrobial lock treatment using 40 mg\/mL<jats:sup>\u22121<\/jats:sup>of tetrasodium EDTA for at least 21 hours could significantly reduce or potentially eradicate CVC-associated bio-films of clinically relevant microorganisms (<jats:italic>Infect Control Hosp Epidemiol<\/jats:italic>2005;26:515-519).<\/jats:p><\/jats:sec>","DOI":"10.1086\/502577","type":"journal-article","created":{"date-parts":[[2006,4,14]],"date-time":"2006-04-14T20:33:48Z","timestamp":1145046828000},"page":"515-519","source":"Crossref","is-referenced-by-count":141,"title":["Tetrasodium EDTA as a Novel Central Venous Catheter Lock Solution Against Biofilm"],"prefix":"10.1017","volume":"26","author":[{"given":"Steven 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