{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:55:17Z","timestamp":1791640517510,"version":"4.3.4"},"reference-count":28,"publisher":"IOP Publishing","issue":"7","license":[{"start":{"date-parts":[[2015,3,19]],"date-time":"2015-03-19T00:00:00Z","timestamp":1426723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2015,3,19]],"date-time":"2015-03-19T00:00:00Z","timestamp":1426723200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Phys. Med. Biol."],"published-print":{"date-parts":[[2015,4,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Complete resection of brain tumors improves life expectancy and quality. Thus, there is a strong need for high-resolution detection and microscopically controlled removal of brain neoplasms. The goal of this study was to test demeclocycline as a contrast enhancer for the intraoperative detection of brain tumors. We have imaged benign and cancerous brain tumors using multimodal confocal microscopy. The tumors investigated included pituitary adenoma, meningiomas, glioblastomas, and metastatic brain cancers. Freshly excised brain tissues were stained in 0.75\u2009mg\u2009ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    aqueous solution of demeclocyline. Reflectance images were acquired at 402\u2009nm. Fluorescence signals were excited at 402\u2009nm and registered between 500 and 540\u2009nm. After imaging, histological sections\u00a0were processed from the imaged specimens and compared to the optical images. Fluorescence images highlighted normal and cancerous brain cells, while reflectance images emphasized the morphology of connective tissue. The optical and histological images were in accordance with each other for all types of tumors investigated. Demeclocyline shows promise as a contrast agent for intraoperative detection of brain tumors.\n                  <\/jats:p>","DOI":"10.1088\/0031-9155\/60\/7\/3003","type":"journal-article","created":{"date-parts":[[2015,3,19]],"date-time":"2015-03-19T10:13:15Z","timestamp":1426759995000},"page":"3003-3011","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Demeclocycline as a contrast agent for detecting brain neoplasms using confocal microscopy"],"prefix":"10.1088","volume":"60","author":[{"given":"Dennis","family":"Wirth","sequence":"first","affiliation":[{"name":"University of Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas W","family":"Smith","sequence":"additional","affiliation":[{"name":"University of Massachusetts Memorial Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Moser","sequence":"additional","affiliation":[{"name":"University of Massachusetts Memorial Medical Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna N","family":"Yaroslavsky","sequence":"additional","affiliation":[{"name":"University of Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2015,3,19]]},"reference":[{"key":"pmb508723bib001","article-title":"Acromegaly and gigantism","author":"Chapman","year":"2014","type":"web-resource"},{"key":"pmb508723bib002","article-title":"Brain and spinal cord tumors in adults","author":"American Cancer Society","year":"2014","type":"other"},{"key":"pmb508723bib003","first-page":"798","type":"journal-article","article-title":"The cytogenesis and pathogenesis of pituitary adenomas","volume":"19","author":"Asa","year":"1998","journal-title":"Endocr. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2014-09-23","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2015-01-20","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2015-03-19","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}