{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T14:14:51Z","timestamp":1778336091670,"version":"3.51.4"},"reference-count":32,"publisher":"IOP Publishing","issue":"28","license":[{"start":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"National Key Basic Research Program of China","award":["2015CB352002"],"award-info":[{"award-number":["2015CB352002"]}]},{"DOI":"10.13039\/501100001809","name":"the Natural Science Foundation of China","doi-asserted-by":"crossref","award":["61535003"],"award-info":[{"award-number":["61535003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Natural Science Foundation of Jiangsu Province","award":["BK20150623"],"award-info":[{"award-number":["BK20150623"]}]},{"name":"the Fundamental Research Funds for the Central Universities","award":["2242017K41010"],"award-info":[{"award-number":["2242017K41010"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Nanotechnology"],"published-print":{"date-parts":[[2018,7,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Single molecule localization microscopy (SMLM) is a powerful tool for imaging biological targets at the nanoscale. In this report, we present SMLM imaging of telomeres and centromeres using fluorescence\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization (FISH). The FISH probes were fabricated by decorating CdSSe\/ZnS quantum dots (QDs) with telomere or centromere complementary DNA strands. SMLM imaging experiments using commercially available peptide nucleic acid (PNA) probes labeled with organic fluorophores were also conducted to demonstrate the advantages of using QDs FISH probes. Compared with the PNA probes, the QDs probes have the following merits. First, the fluorescence blinking of QDs can be realized in aqueous solution or PBS buffer without thiol, which is a key buffer component for organic fluorophores\u2019 blinking. Second, fluorescence blinking of the QDs probe needs only one excitation light (i.e. 405 nm). While fluorescence blinking of the organic fluorophores usually requires two illumination lights, that is, the activation light (i.e. 405 nm) and the imaging light. Third, the high quantum yield, multiple switching times and a good optical stability make the QDs more suitable for long-term imaging. The localization precision achieved in telomeres and centromeres imaging experiments is about 30 nm, which is far beyond the diffraction limit. SMLM has enabled new insights into telomeres or centromeres on the molecular level, and it is even possible to determine the length of telomere and become a potential technique for telomere-related investigation.\n                  <\/jats:p>","DOI":"10.1088\/1361-6528\/aabf72","type":"journal-article","created":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T11:16:12Z","timestamp":1524136572000},"page":"285602","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Single molecule localization imaging of telomeres and centromeres using fluorescence\n                    <i>in situ<\/i>\n                    hybridization and semiconductor quantum dots"],"prefix":"10.1088","volume":"29","author":[{"given":"Le","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenfei","family":"Zong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuyuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruohu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4648-2506","authenticated-orcid":false,"given":"Yiping","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"266","published-online":{"date-parts":[[2018,5,11]]},"reference":[{"key":"nanoaabf72bib1","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1126\/science.1154228","type":"journal-article","article-title":"Video-rate far-field optical nanoscopy dissects synaptic vesicle movement","volume":"320","author":"Volker Westphal","year":"2008","journal-title":"Science"},{"key":"nanoaabf72bib2","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/j.cell.2010.12.002","type":"journal-article","article-title":"Breaking the diffraction barrier: super-resolution imaging of cells","volume":"143","author":"Huang","year":"2010","journal-title":"Cell"},{"key":"nanoaabf72bib3","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1146\/annurev.biochem.77.061906.092014","type":"journal-article","article-title":"Super-resolution fluorescence microscopy","volume":"78","author":"Huang","year":"2009","journal-title":"Annu. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2018-02-01","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2018-04-19","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2018-05-11","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}