{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T14:55:35Z","timestamp":1772290535460,"version":"3.50.1"},"reference-count":34,"publisher":"IOP Publishing","issue":"2","license":[{"start":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T00:00:00Z","timestamp":1541721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T00:00:00Z","timestamp":1541721600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Nanotechnology"],"published-print":{"date-parts":[[2019,1,11]]},"abstract":"Abstract<\/jats:title>\n \n Foodborne pathogens are perpetual threats to human and animal health. Detection of pathogens requires accurate, sensitive, rapid and point-of-care diagnostic assays. In this study, we described a simple and sensitive electrochemiluminescent (ECL) assay to detect the deadly bacteria\n Escherichia coli<\/jats:italic>\n O157:H7 by\n \n \n \n <\/jats:tex-math>\n \n Ru<\/mml:mi>\n \n \n \n \n (<\/mml:mo>\n \n bpy<\/mml:mi>\n <\/mml:mrow>\n )<\/mml:mo>\n <\/mml:mrow>\n <\/mml:mrow>\n \n 3<\/mml:mn>\n <\/mml:mrow>\n <\/mml:msub>\n \n 2<\/mml:mn>\n +<\/mml:mo>\n <\/mml:mrow>\n <\/mml:msup>\n <\/mml:math>\n <\/jats:inline-formula>\n -coated ZnO nanorods arrays (NAs). The\n \n \n \n <\/jats:tex-math>\n \n Ru<\/mml:mi>\n \n \n \n \n (<\/mml:mo>\n \n bpy<\/mml:mi>\n <\/mml:mrow>\n )<\/mml:mo>\n <\/mml:mrow>\n <\/mml:mrow>\n \n 3<\/mml:mn>\n <\/mml:mrow>\n <\/mml:msub>\n \n 2<\/mml:mn>\n +<\/mml:mo>\n <\/mml:mrow>\n <\/mml:msup>\n <\/mml:math>\n <\/jats:inline-formula>\n -coated ZnO NAs were fabricated by immobilizing\n \n \n \n <\/jats:tex-math>\n \n Ru<\/mml:mi>\n \n \n \n \n (<\/mml:mo>\n \n bpy<\/mml:mi>\n <\/mml:mrow>\n )<\/mml:mo>\n <\/mml:mrow>\n <\/mml:mrow>\n \n 3<\/mml:mn>\n <\/mml:mrow>\n <\/mml:msub>\n \n 2<\/mml:mn>\n +<\/mml:mo>\n <\/mml:mrow>\n <\/mml:msup>\n <\/mml:math>\n <\/jats:inline-formula>\n on ZnO NAs with a large specific surface area and good conductivity. An\n \n \n \n <\/jats:tex-math>\n \n Ru<\/mml:mi>\n \n \n \n \n (<\/mml:mo>\n \n bpy<\/mml:mi>\n <\/mml:mrow>\n )<\/mml:mo>\n <\/mml:mrow>\n <\/mml:mrow>\n \n 3<\/mml:mn>\n <\/mml:mrow>\n <\/mml:msub>\n \n 2<\/mml:mn>\n +<\/mml:mo>\n <\/mml:mrow>\n <\/mml:msup>\n <\/mml:math>\n <\/jats:inline-formula>\n -2-(dibutylamino)-ethanol (DBAE) system coated on ZnO NAs exhibits high ECL intensity, rapid response and good stability. This system was further developed as an ECL immunosensor used in the detection of\n E. coli<\/jats:italic>\n O157:H7. The proposed ECL immunosensor exhibits a broad detection range within the scope of 200\u2013100 000 CFU ml\n \u22121<\/jats:sup>\n and quite a low detection limit of 143 CFU ml\n \u22121<\/jats:sup>\n . The high specificity, remarkable reproducibility and good stability offer a sensitive, selective, and convenient pathway for detecting\n E. coli<\/jats:italic>\n O157:H7 in the field of food safety and clinical diagnosis.\n <\/jats:p>","DOI":"10.1088\/1361-6528\/aaea36","type":"journal-article","created":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T18:51:45Z","timestamp":1540234305000},"page":"025501","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Electrochemiluminescent detection of\n Escherichia coli<\/i>\n O157:H7 based on Ru(bpy)\n 3<\/sub>\n 2+<\/sup>\n \/ZnO nanorod arrays"],"prefix":"10.1088","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6191-4429","authenticated-orcid":false,"given":"Danqing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianchan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3466-5169","authenticated-orcid":false,"given":"Weicheng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoqin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"266","published-online":{"date-parts":[[2018,11,9]]},"reference":[{"key":"nanoaaea36bib1","doi-asserted-by":"publisher","first-page":"4818","DOI":"10.1039\/C6CS00693K","type":"journal-article","article-title":"Methods for the detection and identification of pathogenic bacteria: past, present, and future","volume":"46","author":"Varadi","year":"2017","journal-title":"Chem. Soc. Rev."},{"key":"nanoaaea36bib2","doi-asserted-by":"publisher","first-page":"2623","DOI":"10.4315\/0362-028X-68.12.2623","type":"journal-article","article-title":"Economic cost of illness due to Escherichia coli O157 infections in the United States","volume":"68","author":"Frenzen","year":"2005","journal-title":"J. Food Prot."},{"key":"nanoaaea36bib3","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1038\/nature06536","type":"journal-article","article-title":"Global trends in emerging infectious diseases","volume":"451","author":"Jones","year":"2008","journal-title":"Nature"},{"key":"nanoaaea36bib4","doi-asserted-by":"publisher","first-page":"4991","DOI":"10.1039\/C5AN00637F","type":"journal-article","article-title":"Electrochemical nanoparticle-enzyme sensors for screening bacterial contamination in drinking water","volume":"140","author":"Chen","year":"2015","journal-title":"Analyst"},{"key":"nanoaaea36bib5","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.ijfoodmicro.2014.05.019","type":"journal-article","article-title":"Preventing adhesion of Escherichia coli O157:H7 and Salmonella typhimurium LT2 on tomato surfaces via ultrathin polyethylene glycol film","volume":"185","author":"Zhang","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"nanoaaea36bib6","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0167-7012(01)00351-7","type":"journal-article","article-title":"Detection and enumeration of coliforms in drinking water: current methods and emerging approaches","volume":"49","author":"Rompre","year":"2002","journal-title":"J. Microbiol. Methods"},{"key":"nanoaaea36bib7","doi-asserted-by":"publisher","first-page":"3193","DOI":"10.1039\/c2cs15340h","type":"journal-article","article-title":"Nanomaterials for targeted detection and photothermal killing of bacteria","volume":"41","author":"Ray","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"nanoaaea36bib8","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1146\/annurev-food-030216-030216","type":"journal-article","article-title":"Food analysis using organelle DNA and the effects of processing on assays","volume":"8","author":"Caldwell","year":"2017","journal-title":"Annu. Rev. Food. Sci. Technol."},{"key":"nanoaaea36bib9","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1146\/annurev-anchem-071213-020249","type":"journal-article","article-title":"Nano\/micro and spectroscopic approaches to food pathogen detection","volume":"7","author":"Cho","year":"2014","journal-title":"Annu. Rev. Anal. Chem."},{"key":"nanoaaea36bib10","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1146\/annurev-anchem-061516-045310","type":"journal-article","article-title":"Nanosensor technology applied to living plant systems","volume":"10","author":"Kwak","year":"2017","journal-title":"Annu. Rev. Anal. Chem."},{"key":"nanoaaea36bib11","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1146\/annurev-anchem-071213-020051","type":"journal-article","article-title":"Cell-based microarrays for in vitro toxicology","volume":"8","author":"Wegener","year":"2015","journal-title":"Annu. Rev. Anal. Chem."},{"key":"nanoaaea36bib12","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1016\/j.bios.2016.09.029","type":"journal-article","article-title":"A nuclease-assisted label-free aptasensor for fluorescence turn-on detection of ATP based on the in situ formation of copper nanoparticles","volume":"87","author":"Song","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"nanoaaea36bib13","doi-asserted-by":"publisher","first-page":"9042","DOI":"10.1039\/C7CC04300G","type":"journal-article","article-title":"Recent trends in electrochemiluminescence aptasensors and their applications","volume":"53","author":"Nasiri Khonsari","year":"2017","journal-title":"Chem. Commun."},{"key":"nanoaaea36bib14","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.bios.2013.11.011","type":"journal-article","article-title":"Current trends in the development of the electrochemiluminescent immunosensors","volume":"54","author":"Muzyka","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"nanoaaea36bib15","doi-asserted-by":"publisher","first-page":"3117","DOI":"10.1039\/C5CS00086F","type":"journal-article","article-title":"Recent advances in electrochemiluminescence","volume":"44","author":"Liu","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"nanoaaea36bib16","doi-asserted-by":"publisher","first-page":"3911","DOI":"10.1007\/s00216-015-8557-x","type":"journal-article","article-title":"A review of electrochemiluminescence (ECL) in and for microfluidic analytical devices","volume":"407","author":"Kirschbaum","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"nanoaaea36bib17","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.aca.2013.01.045","type":"journal-article","article-title":"Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination","volume":"770","author":"Ballesta-Claver","year":"2013","journal-title":"Anal. Chim. Acta"},{"key":"nanoaaea36bib18","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1016\/j.bios.2012.10.005","type":"journal-article","article-title":"A novel electrochemiluminescence ethanol biosensor based on tris(2,2\u2019-bipyridine) ruthenium (II) and alcohol dehydrogenase immobilized in graphene\/bovine serum albumin composite film","volume":"41","author":"Gao","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"nanoaaea36bib19","doi-asserted-by":"publisher","first-page":"3549","DOI":"10.1007\/s00216-012-6510-9","type":"journal-article","article-title":"Highly porous magnetite\/graphene nanocomposites for a solid-state electrochemiluminescence sensor on paper-based chips","volume":"405","author":"Xu","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"nanoaaea36bib20","doi-asserted-by":"publisher","first-page":"4745","DOI":"10.1002\/adma.201201931","type":"journal-article","article-title":"Electrogenerated chemiluminescence of metal-organic complex nanowires: reduced graphene oxide enhancement and biosensing application","volume":"24","author":"Li","year":"2012","journal-title":"Adv. Mater."},{"key":"nanoaaea36bib21","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1039\/C1AN15942A","type":"journal-article","article-title":"Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels","volume":"137","author":"Zhang","year":"2012","journal-title":"Analyst"},{"key":"nanoaaea36bib22","doi-asserted-by":"publisher","first-page":"5892","DOI":"10.1021\/ac100435z","type":"journal-article","article-title":"Enhanced solid-state electrochemiluminescence of tris(2,2\u2032-bipyridyl) ruthenium (II) incorporated into electrospun nanofibrous mat","volume":"82","author":"Shan","year":"2010","journal-title":"Anal. Chem."},{"key":"nanoaaea36bib23","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.talanta.2013.01.007","type":"journal-article","article-title":"A solid-state electrochemiluminescence sensing platform for detection of catechol based on novel luminescent composite nanofibers","volume":"107","author":"Wang","year":"2013","journal-title":"Talanta"},{"key":"nanoaaea36bib24","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1002\/celc.201500329","type":"journal-article","article-title":"Vertically ordered silica mesochannel modified bipolar electrode for electrochemiluminescence imaging analysis","volume":"3","author":"Guo","year":"2016","journal-title":"Chemelectrochem"},{"key":"nanoaaea36bib25","first-page":"48","type":"other","article-title":"Two orders-of-magnitude enhancement in the electrochemiluminescence of Ru(bpy)32+ by vertically ordered silica mesochannels","volume":"886","author":"Zhou","year":"2015"},{"key":"nanoaaea36bib26","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1039\/C6EE02265K","type":"journal-article","article-title":"Noble metal\u2013metal oxide nanohybrids with tailored nanostructures for efficient solar energy conversion, photocatalysis and environmental remediation","volume":"10","author":"Liu","year":"2017","journal-title":"Energy Environ. Sci."},{"key":"nanoaaea36bib27","doi-asserted-by":"publisher","first-page":"95","DOI":"10.3390\/nano7050095","type":"journal-article","article-title":"One-dimensional electron transport layers for perovskite solar cells","volume":"7","author":"Thakur","year":"2017","journal-title":"Nanomaterials"},{"key":"nanoaaea36bib28","doi-asserted-by":"publisher","first-page":"26529","DOI":"10.1021\/jp410692y","type":"journal-article","article-title":"CdTe quantum dots encapsulated ZnO nanorods for highly efficient photoelectrochemical degradation of phenols","volume":"117","author":"Liu","year":"2013","journal-title":"J. Phys. Chem. C"},{"key":"nanoaaea36bib29","doi-asserted-by":"publisher","first-page":"3762","DOI":"10.1002\/smll.201500527","type":"journal-article","article-title":"Continuous microfluidic self-assembly of hybrid janus-like vesicular motors: autonomous propulsion and controlled release","volume":"11","author":"Wang","year":"2015","journal-title":"Small"},{"key":"nanoaaea36bib30","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/s12010-014-0850-1","type":"journal-article","article-title":"Luminol-based chemiluminescent signals: clinical and non-clinical application and future uses","volume":"173","author":"Khan","year":"2014","journal-title":"Appl. Biochem. Biotechnol."},{"key":"nanoaaea36bib31","doi-asserted-by":"publisher","first-page":"8262","DOI":"10.1002\/chem.201406002","type":"journal-article","article-title":"Photoisomerisation in aminoazobenzene-substituted ruthenium(II) tris(bipyridine) complexes: influence of the conjugation pathway","volume":"21","author":"Amar","year":"2015","journal-title":"Chem. Eur. J."},{"key":"nanoaaea36bib32","doi-asserted-by":"publisher","first-page":"1579","DOI":"10.1016\/j.elecom.2009.05.059","type":"journal-article","article-title":"Mechanism for inhibition of Ru(bpy)32+ \/DBAE electrochemiluminescence system by dopamine","volume":"11","author":"Xue","year":"2009","journal-title":"Electrochem. Commun."},{"key":"nanoaaea36bib33","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.trac.2013.06.012","type":"journal-article","article-title":"Electrochemiluminescence quenching of tris(2,2\u2032-bipyridyl)ruthenium","volume":"51","author":"Huang","year":"2013","journal-title":"TRAC Trends. Anal. Chem."},{"key":"nanoaaea36bib34","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.aca.2015.11.047","type":"journal-article","article-title":"Sensitive and versatile electrogenerated chemiluminescence biosensing platform for protein kinase based on Ru(bpy)32+ functionalized gold nanoparticles mediated signal transduction","volume":"906","author":"Dong","year":"2016","journal-title":"Anal. Chim. Acta"}],"container-title":["Nanotechnology"],"original-title":[],"link":[{"URL":"http:\/\/stacks.iop.org\/0957-4484\/30\/i=2\/a=025501?key=crossref.f27543c3beacfc26dad1639386f0c7f8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"http:\/\/stacks.iop.org\/0957-4484\/30\/i=2\/a=025501\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"http:\/\/stacks.iop.org\/0957-4484\/30\/i=2\/a=025501\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/stacks.iop.org\/0957-4484\/30\/i=2\/a=025501?key=crossref.f27543c3beacfc26dad1639386f0c7f8","content-type":"text\/html","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T13:56:55Z","timestamp":1772287015000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1361-6528\/aaea36"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,9]]},"references-count":34,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,11,9]]},"published-print":{"date-parts":[[2019,1,11]]}},"URL":"https:\/\/doi.org\/10.1088\/1361-6528\/aaea36","relation":{},"ISSN":["0957-4484","1361-6528"],"issn-type":[{"value":"0957-4484","type":"print"},{"value":"1361-6528","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,9]]},"assertion":[{"value":"Electrochemiluminescent detection of\n Escherichia coli\n O157:H7 based on Ru(bpy)\n 3\n 2+\n \/ZnO nanorod arrays","name":"article_title","label":"Article Title"},{"value":"Nanotechnology","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2018 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2018-08-13","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2018-10-22","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2018-11-09","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}