{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T13:23:40Z","timestamp":1766237020232,"version":"3.48.0"},"reference-count":28,"publisher":"IOP Publishing","issue":"29","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":4,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"tdm","delay-in-days":4,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Nanotechnology"],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We report the fabrication of silver nanoparticles (AgNPs) surface functionalized with gelatin at different concentrations (G10\/G20\/G40 AgNPs) with an average particle size of \u223c200 nm, bioconjugated with antisera antibodies (AsAbs) of the major and clinically significant blood groups (CSBGs) at different titres from neat to 1:128. Bioconjugation using ionic interaction at pH 7.4 enabled \u2018end-on\u2019 configuration, with the \u2013NH2 group of the antibody free for interaction with the red blood cell antigen, as confirmed by Fourier transform infrared spectroscopy. The tube agglutination test (TAT) revealed optimum agglutination with G20NPs, while SDS PAGE confirmed the optimal titre as 1:8 for the major blood groups A, B, AB and O. Bioconjugated AgNPs coated onto microtitre assay plates with the major blood groups and CSBGs to enable simultaneous identification, were validated against the TAT on 400 random blood samples for the major blood groups and revealed high accuracy (95%). While similar accuracy was seen for most of the CSBGs with only false negatives, the method was not found to be suitable for the Kell, Kidd and Duffy groups. The absence of false positives reflects high safety, and eliminates the risk of a mismatched blood transfusion. The method uses diluted blood and hence could enable point-of-care detection. The significantly lower AsAb requirement also provides a cost advantage.<\/jats:p>","DOI":"10.1088\/1361-6528\/ab83b9","type":"journal-article","created":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T18:30:17Z","timestamp":1585247417000},"page":"295102","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Fabrication of gelatin functionalized silver nanoparticles for blood group profiling"],"prefix":"10.1088","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5247-9071","authenticated-orcid":false,"given":"Shweta S","family":"Chawla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajit C","family":"Gorakshakar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanjaksha K","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manisha R","family":"Madkaikar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Padma V","family":"Devarajan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"266","published-online":{"date-parts":[[2020,5,5]]},"reference":[{"year":"2018","author":"Ramzan","key":"nanoab83b9bib1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-813661-4.00004-3","type":"book"},{"key":"nanoab83b9bib2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1556-276X-6-98","type":"journal-article","article-title":"Core shell hybrids based on noble metal nanoparticles and conjugated polymers: synthesis and characterization","volume":"6","author":"Fratoddi","year":"2011","journal-title":"Nanoscale Res. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2020-01-22","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2020-03-26","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2020-05-05","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}