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Additionally, the hybrid nanoflowers are self-assembled by cholesterol oxidase (ChOx) and copper phosphate (Cu\n                    <jats:sub>3<\/jats:sub>\n                    (PO\n                    <jats:sub>4<\/jats:sub>\n                    )\n                    <jats:sub>2<\/jats:sub>\n                    \u00b73H\n                    <jats:sub>2<\/jats:sub>\n                    O). Then a new strategy for cholesterol determination is developed based on MOF@MnO\n                    <jats:sub>2<\/jats:sub>\n                    nanocomposites and hybrid nanoflowers. Cholesterol is oxidized under the catalysis of hybrid nanoflowers to yield H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , which further reduces MnO\n                    <jats:sub>2<\/jats:sub>\n                    nanosheets into Mn\n                    <jats:sup>2+<\/jats:sup>\n                    . Hence, the fluorescence recovery of Tb\u2013MOFs is positively correlated to the concentration of cholesterol in the range of 10 to 360\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M. The limit of detection (LOD) of cholesterol is 1.57\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M. On the other hand, the hierarchical and confined structure of ChOx\u2013inorganic hybrid nanoflowers greatly improve the stability of the enzyme. The activity of hybrid nanoflowers remains at a high level for one week when stored at room temperature. Moreover, the hybrid nanoflowers can be collected by centrifugation and reused. The activity of hybrid nanoflowers can continue at a high level for five cycles of determination. Therefore, it can be concluded that the hybrid nanoflowers are more stable and more economic than free enzymes, and they show a similar sensitivity and specificity to cholesterol compared with free ChOx. Finally, this strategy has been further validated for the determination of cholesterol in serum samples with satisfactory recoveries.\n                  <\/jats:p>","DOI":"10.1088\/1361-6528\/abf692","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T18:35:42Z","timestamp":1617993342000},"page":"315502","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["MOF@MnO\n                    <sub>2<\/sub>\n                    nanocomposites prepared using\n                    <i>in situ<\/i>\n                    method and recyclable cholesterol oxidase\u2013inorganic hybrid nanoflowers for cholesterol determination"],"prefix":"10.1088","volume":"32","author":[{"given":"Dawei","family":"Xu","sequence":"first","affiliation":[{"name":"Qufu Normal University"},{"name":"Qufu Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Li","sequence":"additional","affiliation":[{"name":"Qufu Normal University"},{"name":"Qufu Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqiu","family":"Zi","sequence":"additional","affiliation":[{"name":"Qufu Normal University"},{"name":"Qufu Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dafeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Physical and Chemical Testing, Shandong Center for Disease Control and Prevention, Jinan 250014,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5394-9813","authenticated-orcid":false,"given":"Fei","family":"Qu","sequence":"additional","affiliation":[{"name":"Qufu Normal University"},{"name":"Qufu Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian-En","family":"Zhao","sequence":"additional","affiliation":[{"name":"Qufu Normal University"},{"name":"Qufu Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2021,5,10]]},"reference":[{"key":"nanoabf692bib1","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.snb.2015.02.045","type":"journal-article","article-title":"Electrochemical and optical properties of a conducting polymer and its use in a novel biosensor for the detection of cholesterol","volume":"212","author":"Soylemez","year":"2015","journal-title":"Sensor Actuat. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2020-11-08","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2021-04-09","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2021-05-10","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}