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Conventional detection techniques rely on bulky instrumentation and complex workflows that hinder point-of-care implementation. Here, we report a portable smartphone-based fluorescence sensing platform specifically designed to address the limitations of existing exosome assays in portability, quantitative robustness, and operational simplicity. Exosome capture was achieved through a CD63-specific aptamer, followed by molecular recognition using a FAM-labeled, tumor-specific aptamer. A miniaturized optical readout device integrating an excitation unit, optical filters, and an imaging module enables fluorescence acquisition and quantitative analysis without large laboratory equipment. The system completes exosome detection within 45 min and achieves a limit of detection of 4.58 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    particles\/ml. Clinical sample analysis preliminarily demonstrates the feasibility of serum exosome detection using this portable platform, suggesting its potential utility for rapid, on-site biomedical screening.\n                  <\/jats:p>","DOI":"10.1088\/1361-6528\/ae9169","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T22:48:27Z","timestamp":1785278907000},"page":"315502","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A portable smartphone-based fluorescence biosensing strategy for exosome detection"],"prefix":"10.1088","volume":"37","author":[{"given":"Sijie","family":"Zhang","sequence":"first","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongju","family":"Shi","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemeng","family":"Li","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wu","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7113-1951","authenticated-orcid":true,"given":"Kuo","family":"Yang","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruohu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6848-6009","authenticated-orcid":true,"given":"Shenfei","family":"Zong","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1262-5342","authenticated-orcid":true,"given":"Zhuyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4648-2506","authenticated-orcid":true,"given":"Yiping","family":"Cui","sequence":"additional","affiliation":[{"name":"Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"nanoae9169bib1","doi-asserted-by":"publisher","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","type":"journal-article","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"nanoae9169bib2","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nri2567","type":"journal-article","article-title":"Membrane vesicles as conveyors of immune responses","volume":"9","author":"Th\u00e9ry","year":"2009","journal-title":"Nat. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2026-03-17","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-07-28","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-08-06","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}