{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T05:09:46Z","timestamp":1775884186650,"version":"3.50.1"},"reference-count":51,"publisher":"IOP Publishing","issue":"6","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100017395","name":"University of Richmond","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100017395","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Phys. Biol."],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fibrin fibers are important structural elements in blood coagulation. They form a mesh network that acts as a scaffold and imparts mechanical strength to the clot. A review of published work measuring the mechanics of fibrin fibers reveals a range of values for fiber extensibility. This study investigates fibrinogen concentration as a variable responsible for variability in fibrin mechanics. It expands previous work to describe the modulus, strain hardening, extensibility, and the force required for fiber failure when fibers are formed with different fibrinogen concentrations using lateral force atomic force microscopy. Analysis of the mechanical properties showed fibers formed from 1 mg ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 2 mg ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    fibrinogen had significantly different mechanical properties. To help clarify our findings we developed two behavior profiles to describe individual fiber mechanics. The first describes a fiber with low initial modulus and high extensible, that undergoes significant strain hardening, and has moderate strength. Most fibers formed with 1 mg ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    fibrinogen had this behavior profile. The second profile describes a fiber with a high initial modulus, minimal strain hardening, high strength, and low extensibility. Most fibrin fibers formed with 2 mg ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    fibrinogen were described by this second profile. In conclusion, we see a range of behaviors from fibers formed from native fibrinogen molecules but various fibrinogen concentrations. Potential differences in fiber formation are investigated with SEM. It is likely this range of behaviors also occurs\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Understanding the variability in mechanical properties could contribute to a deeper understanding of pathophysiology of coagulative disorders.\n                  <\/jats:p>","DOI":"10.1088\/1478-3975\/ad899f","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T19:04:50Z","timestamp":1729537490000},"page":"066003","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Variability in individual native fibrin fiber mechanics"],"prefix":"10.1088","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1056-0859","authenticated-orcid":true,"given":"Christine C","family":"Helms","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"266","published-online":{"date-parts":[[2024,10,30]]},"reference":[{"key":"pbad899fbib1","doi-asserted-by":"publisher","first-page":"8272","DOI":"10.1039\/D0SM00916D","article-title":"Molecular packing structure of fibrin fibers resolved by x-ray scattering and molecular modeling","volume":"16","author":"Jansen","year":"2020","journal-title":"Soft Matter"},{"key":"pbad899fbib2","doi-asserted-by":"publisher","first-page":"2018","DOI":"10.1016\/j.bpj.2010.04.059","article-title":"Nanostructure of the fibrin clot","volume":"99","author":"Yeromonahos","year":"2010","journal-title":"Biophys. 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