{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:06:29Z","timestamp":1794002789723,"version":"4.8.0"},"reference-count":43,"publisher":"IOP Publishing","issue":"4","license":[{"start":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T00:00:00Z","timestamp":1502928000000},"content-version":"vor","delay-in-days":16,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T00:00:00Z","timestamp":1502928000000},"content-version":"tdm","delay-in-days":16,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"Central Silk Board, Bengaluru, India","award":["CSB-31\/2\/PROJECT\/2010-11-RCS"],"award-info":[{"award-number":["CSB-31\/2\/PROJECT\/2010-11-RCS"]}]},{"name":"Department of Biotechnology, New Delhi","award":["BT\/PR14042\/MED\/30\/338\/2010"],"award-info":[{"award-number":["BT\/PR14042\/MED\/30\/338\/2010"]}]},{"name":"Department of Biotechnology , Ministry of Science and Technology"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Mater."],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The present study describes the fabrication of polyaniline-silk fibroin (PASF) nanocomposite-based nerve conduits and their subsequent implantation in a rat sciatic nerve injury model for peripheral nerve regeneration. This is the first\n                    <jats:italic>in vivo<\/jats:italic>\n                    study of polyaniline-based nerve conduits describing the safety and efficacy of the conduits in treating peripheral nerve injuries. The nanocomposite was synthesized by electrospinning a mixture of silk fibroin protein and polyaniline wherein the silk nanofibers were observed to be uniformly coated with polyaniline nanoparticles. Tubular shaped nerve conduits were subsequently formed by multiple rolling of the electrospun sheet over a stainless steel mandrel. The conduits were characterized\n                    <jats:italic>in vitro<\/jats:italic>\n                    for their physico-chemical properties as well as their compatibility with rat Schwann cells. Upon implantation in a 10 mm sciatic nerve injury model, the conduits were evaluated for their neuro-regenerative potential through extensive electrophysiological studies and monitoring of gait pattern over a course of 12 months. Gross examination, histological and ultra-structure analyses of the conduits and the regenerated nerve were also performed to evaluate morphological regeneration of transected nerve. PASF nanocomposite conduits seeded with Schwann cell (cell seeded PASF) exhibited excellent nerve conduction velocity (NCV) (50 m s\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), compound muscle action potential (CMAP) (12.8 mV), motor unit potential (MUP) (124\n                    <jats:italic>\u03bc<\/jats:italic>\n                    V), growth of healthy tissue along the nerve gap and thick myelination of axons 12 months after implantation indicating enhanced neuro-regeneration. The excellent functional recovery achieved by animals implanted with cell seeded PASF conduits (86.2% NCV; 80.00% CMAP; 76.07% MUP) are superior to outcomes achieved previously with similar electrically conductive conduits. We believe that the present study would encourage further research in developing electrically active neural implants using synthetic conducting polymers and the\n                    <jats:italic>in vivo<\/jats:italic>\n                    applications of the same.\n                  <\/jats:p>","DOI":"10.1088\/1748-605x\/aa7802","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T10:57:50Z","timestamp":1497956270000},"page":"045025","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":79,"title":["Electrospun silk-polyaniline conduits for functional nerve regeneration in rat sciatic nerve injury model"],"prefix":"10.1088","volume":"12","author":[{"given":"Suradip","family":"Das","sequence":"first","affiliation":[{"name":"Indian Institute of Technology Guwahati"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manav","family":"Sharma","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Guwahati"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhiren","family":"Saharia","sequence":"additional","affiliation":[{"name":"Saharia\u2019s Path Lab & Blood Bank"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kushal Konwar","family":"Sarma","sequence":"additional","affiliation":[{"name":"College of Veterinary Sciences"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M","family":"Muir","sequence":"additional","affiliation":[{"name":"University of Cambridge"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utpal","family":"Bora","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Guwahati"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2017,8,17]]},"reference":[{"key":"bmmaa7802bib1","doi-asserted-by":"publisher","first-page":"24423","DOI":"10.1039\/c3ra44808h","type":"journal-article","article-title":"Fabrication of conducting polyaniline microspheres using droplet microfluidics","volume":"3","author":"Beesabathuni","year":"2013","journal-title":"RSC Adv."},{"key":"bmmaa7802bib2","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.jcis.2009.08.044","type":"journal-article","article-title":"Coating of multiwalled carbon nanotubes with polymer nanospheres through microemulsion polymerization","volume":"340","author":"Reddy","year":"2009","journal-title":"J. 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