{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:43:03Z","timestamp":1783687383563,"version":"3.55.0"},"reference-count":68,"publisher":"IOP Publishing","issue":"6","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"Canadian Institutes of Health Research (CIHR). Institute of Musculoskeletal Health and Arthritis","award":["#84368 and #111233"],"award-info":[{"award-number":["#84368 and #111233"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Mater."],"published-print":{"date-parts":[[2018,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Engineered adipose tissues are developed for their use as substitutes for tissue replacement in reconstructive surgery. To ensure a timely perfusion of the grafted substitutes, different strategies can be used such as the incorporation of an endothelial component. In this study, we engineered human adipose tissue substitutes comprising of functional adipocytes as well as a natural extracellular matrix using the self-assembly approach, without the use of exogenous scaffolding elements. Human microvascular endothelial cells (hMVECs) were incorporated during tissue production\n                    <jats:italic>in vitro<\/jats:italic>\n                    and we hypothesized that their presence would favor the early connection with the host vascular network translating into functional enhancement after implantation into nude mice in comparison to the substitutes that were not enriched in hMVECs.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , no significant differences were observed between the substitutes in terms of histological aspects. After implantation, both groups presented numerous adipocytes and an abundant matrix in addition to the presence of host capillaries within the grafts. The substitutes thickness and volume were not significantly different between groups over the short-term time course of 14 days (d). For the microvascularized adipose tissues, human CD31 staining revealed a human capillary network connecting with the host microvasculature as early as 3 d after grafting. The detection of murine red blood cells within human CD31+ structures confirmed the functionality of the human capillary network. By analyzing the extent of the global vascularization achieved, a tendency towards increased total capillary network surface and volume was revealed for prevascularized tissues over 14 d. Therefore, applying this strategy on thicker reconstructed adipose tissues with rate-limiting oxygen diffusion might procure added benefits and prove useful to provide voluminous substitutes for patients suffering from adipose tissue loss or defects.\n                  <\/jats:p>","DOI":"10.1088\/1748-605x\/aadff7","type":"journal-article","created":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T10:16:14Z","timestamp":1538475374000},"page":"065013","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Short-term post-implantation dynamics of\n                    <i>in vitro<\/i>\n                    engineered human microvascularized adipose tissues"],"prefix":"10.1088","volume":"13","author":[{"given":"Maryse","family":"Proulx","sequence":"first","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Mayrand","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Vincent","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Boisvert","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Aubin","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Trottier","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1851-5112","authenticated-orcid":false,"given":"Julie","family":"Fradette","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX"},{"name":"CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center"},{"name":"Universit\u00e9 Laval"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2018,10,1]]},"reference":[{"key":"bmmaadff7bib1","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.addr.2011.03.004","type":"journal-article","article-title":"Vascularization is the key challenge in tissue engineering","volume":"63","author":"Novosel","year":"2011","journal-title":"Adv. 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