{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:59:18Z","timestamp":1791687558307,"version":"4.3.4"},"reference-count":39,"publisher":"IOP Publishing","issue":"5","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical research (ICMR), India","doi-asserted-by":"crossref","award":["5\/3\/8\/35\/ITR-F\/2022"],"award-info":[{"award-number":["5\/3\/8\/35\/ITR-F\/2022"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Mater."],"published-print":{"date-parts":[[2023,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In this study, chitosan-gelatin-monetite (CGM)-based electrospun scaffolds have been developed that closely mimicked the microstructure and chemical composition of the extracellular matrix of natural bone. CGM-based nanofibrous composite scaffolds were prepared with the help of the electrospinning technique, post-cross-linked using ethyl(dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide solution to improve their stability in an aqueous environment. The prepared chitosan\/gelatin (CG) scaffold showed an average fiber diameter of 308 \u00b1 17 nm, whereas 5 and 7 wt% monetite containing CGM<jats:sub>5<\/jats:sub> and CGM<jats:sub>7<\/jats:sub> scaffolds, exhibited an average fiber diameter of 287 \u00b1 13 and 265 \u00b1 9 nm, respectively, revealing the fine distribution of monetite particles on the fibrous surface. The distribution of monetite nanoparticles onto the CG nanofibrous surface was confirmed using x-ray diffraction, Fourier transform infrared, and EDAX. Moreover, the addition of 7 wt% monetite into the CG electrospun matrix increased their ultimate tensile strength from 7.62 \u00b1 0.13 MPa in the CG scaffold to 14.34 \u00b1 0.39 MPa in the CGM<jats:sub>7<\/jats:sub> scaffold. Simulated body fluid study and staining with alizarin red S (ARS) confirmed the higher mineralization ability of monetite-containing scaffolds compared to that revealed by the CG scaffold. The monetite incorporation into the CG matrix improved its osteogenic properties, including pre-osteoblast MG-63 cell adhesion, proliferation, and differentiation, when seeded with the cells. A higher degree of cellular adhesion, spreading, and migration was observed on the monetite-incorporated CG scaffold than that on the CG scaffold. From 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) MTT assay, alkaline phosphatase activity, ARS staining, and immunocytochemistry study, the cultured cells discovered a more conducive microenvironment to proliferate and subsequently differentiate into osteoblast lineage in contact with CGM<jats:sub>7<\/jats:sub> nanofibers rather than that in CGM<jats:sub>0<\/jats:sub> and CGM<jats:sub>5<\/jats:sub>. <jats:italic>In-vitro<\/jats:italic> results indicated that electrospun CGM-based composite scaffolds could be used as a potential candidate to repair and regenerate new bone tissues.<\/jats:p>","DOI":"10.1088\/1748-605x\/ace7a3","type":"journal-article","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T18:27:08Z","timestamp":1689359228000},"page":"055006","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Preparation, characterization, and bioactivity of reinforced monetite with chitosan-gelatin electrospun composite scaffold for bone tissue engineering"],"prefix":"10.1088","volume":"18","author":[{"given":"Yogendra Pratap","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1423-521X","authenticated-orcid":true,"given":"ShivDutt","family":"Purohit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0125-0381","authenticated-orcid":true,"given":"Mukesh Kumar","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0181-6197","authenticated-orcid":true,"given":"Rakesh","family":"Bhaskar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Soo","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9370-8862","authenticated-orcid":true,"given":"Sudip","family":"Dasgupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"bmmace7a3bib1","doi-asserted-by":"publisher","first-page":"4110","DOI":"10.1016\/j.bioactmat.2021.03.043","type":"journal-article","article-title":"Bone physiological microenvironment and healing mechanism: basis for future bone-tissue engineering scaffolds","volume":"6","author":"Zhu","year":"2021","journal-title":"Bioact. 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