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Furthermore, the derivative CNI3-2 exhibited significantly enhanced potency compared to CNI3, achieving 50% cytotoxicity at 75 \u00b5M in 3D spheroids. In conclusion, by reproducing the drug resistance characteristics observed\n                    <jats:italic>in vivo<\/jats:italic>\n                    , this 3D tumor spheroid array chip provides a more clinically predictive model than traditional 2D methods, offering a robust solution for high-throughput screening that potentially bridges the gap between preclinical\n                    <jats:italic>in vitro<\/jats:italic>\n                    testing and clinical therapeutic outcomes.\n                  <\/jats:p>","DOI":"10.1088\/1748-605x\/ae79c5","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T22:53:37Z","timestamp":1780959217000},"page":"035032","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A scalable and cost-effective 3D tumor spheroid array chip for distortion-free optical monitoring and drug screening"],"prefix":"10.1088","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1995-0734","authenticated-orcid":true,"given":"Yang","family":"Lu","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3934-5911","authenticated-orcid":true,"given":"Haoran","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8018-322X","authenticated-orcid":true,"given":"Xiaoying","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"},{"name":"Shanghai Frontiers Science Research Center for Druggability of Cardiovascular noncoding RNA, Institute for Frontier Medical Technology, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanxing","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atta Ur Rehman","family":"Khan","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2704-1223","authenticated-orcid":true,"given":"Rui","family":"Xi","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinan","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"bmmae79c5bib1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201600505","type":"journal-article","article-title":"Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read\u2010outs","volume":"12","author":"Santo","year":"2017","journal-title":"Biotechnol. 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