{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:40:58Z","timestamp":1792478458538,"version":"4.5.0"},"reference-count":54,"publisher":"IOP Publishing","issue":"5","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":19,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/questions\/alternative-author-rights-policies\/"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/questions\/alternative-author-rights-policies\/"}],"funder":[{"name":"NHMRC-ARISTOCRAT","award":["MRF2017213"],"award-info":[{"award-number":["MRF2017213"]}]},{"name":"Australian National Fabrication Facility"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Mater."],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Clinically relevant cell numbers are essential for many tissue engineering applications, requiring scalable expansion platforms. Microcarrier-based bioreactor systems address this demand; however, conventional non-dissolvable carriers require harsh enzymatic or mechanical detachment, reducing cell viability and integrity. Although dissolvable microcarriers improve recovery, most are designed only for harvesting and lack functionality for downstream tissue engineering. Here, we present a sequential dual-crosslinkable alginate-methacrylate-RGD microsphere system (Alg\u2013MR\u2013MS) that integrates scalable cell expansion with scaffold formation in a single platform. The workflow involves: (1) microsphere fabrication via calcium-mediated ionic crosslinking; (2) three-dimensional cell expansion in a microcarrier culture system; (3) controlled phase change through calcium chelation with sodium citrate, enabling efficient cell recovery while preserving viability and phenotype; and (4) photocrosslinking to form a stable, cell-laden hydrogel suitable for bioprinting and tissue engineering. This sequential dual-crosslinking strategy supports expansion, dissolution, and structural stabilisation within one system. Human adipose-derived stem cells cultured on Alg\u2013MR\u2013MS showed \u223c5-fold increased metabolic activity over 7 d, comparable to 2D controls, with high viability and strong attachment. Compared with other dissolvable carriers, Alg\u2013MR\u2013MS offers efficient expansion, rapid phase transition, and simplified single-step fabrication, bridging biomanufacturing and functional tissue engineering.<\/jats:p>","DOI":"10.1088\/1748-605x\/aeb214","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:52:17Z","timestamp":1791499937000},"page":"055022","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Alginate\u2013methacrylate\u2013RGD microspheres (Alg\u2013MR\u2013MS) for cell expansion and delivery in tissue engineering"],"prefix":"10.1088","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6137-8320","authenticated-orcid":true,"given":"Alexandre Xavier","family":"Mendes","sequence":"first","affiliation":[{"name":"Swinburne University of Technology"},{"name":"St Vincent\u2019s Hospital Melbourne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6418-3323","authenticated-orcid":false,"given":"Serena","family":"Duchi","sequence":"additional","affiliation":[{"name":"St Vincent\u2019s Hospital Melbourne"},{"name":"University of Melbourne, St Vincent\u2019s Hospital Melbourne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1076-6419","authenticated-orcid":true,"given":"Carmine","family":"Onofrillo","sequence":"additional","affiliation":[{"name":"St Vincent\u2019s Hospital Melbourne"},{"name":"University of Melbourne, St Vincent\u2019s Hospital Melbourne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"University of Wollongong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3428-1501","authenticated-orcid":false,"given":"Sanjeev","family":"Gambhir","sequence":"additional","affiliation":[{"name":"University of Wollongong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2044-4197","authenticated-orcid":false,"given":"Adriana Teixeira","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Swinburne University of Technology"},{"name":"St Vincent\u2019s Hospital Melbourne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1690-9505","authenticated-orcid":false,"given":"Cathal","family":"O\u2019Connell","sequence":"additional","affiliation":[{"name":"St Vincent\u2019s Hospital Melbourne"},{"name":"RMIT University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3019-7559","authenticated-orcid":true,"given":"Steven","family":"Posniak","sequence":"additional","affiliation":[{"name":"University of Wollongong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1095-1566","authenticated-orcid":false,"given":"Payal","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Health and Human Sciences, Macquarie University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0381-7273","authenticated-orcid":false,"given":"Gordon G","family":"Wallace","sequence":"additional","affiliation":[{"name":"University of Wollongong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3522-7374","authenticated-orcid":false,"given":"Peter F","family":"Choong","sequence":"additional","affiliation":[{"name":"University of Melbourne, St Vincent\u2019s Hospital Melbourne"},{"name":"St Vincent\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8320-3852","authenticated-orcid":true,"given":"Simon E","family":"Moulton","sequence":"additional","affiliation":[{"name":"Swinburne University of Technology"},{"name":"St Vincent\u2019s Hospital Melbourne"},{"name":"Iverson Health Innovation Research Institute, Swinburne University of Technology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"bmmaeb214bib1","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.biomaterials.2018.07.016","type":"journal-article","article-title":"Large-scale production of stem cells utilizing microcarriers: a biomaterials engineering perspective from academic research to commercialized products","volume":"181","author":"Tavassoli","year":"2018","journal-title":"Biomaterials"},{"key":"bmmaeb214bib2","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1016\/j.biotechadv.2013.03.006","type":"journal-article","article-title":"Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: achievements and future direction","volume":"31","author":"Chen","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"bmmaeb214bib3","doi-asserted-by":"publisher","DOI":"10.1002\/biot.202000048","type":"journal-article","article-title":"Dissolvable gelatin-based microcarriers generated through droplet microfluidics for expansion and culture of mesenchymal stromal cells","volume":"16","author":"Ng","year":"2021","journal-title":"Biotechnol. J."},{"key":"bmmaeb214bib4","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2019.109782","type":"journal-article","article-title":"Attachment and detachment strategies in microcarrier-based cell culture technology: a comprehensive review","volume":"103","author":"Derakhti","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"bmmaeb214bib5","doi-asserted-by":"publisher","first-page":"2937","DOI":"10.1002\/jbm.b.34624","type":"journal-article","article-title":"Scale-up manufacturing of gelatin-based microcarriers for cell therapy","volume":"108","author":"Dosta","year":"2020","journal-title":"J. Biomed. Mater. Res. B"},{"key":"bmmaeb214bib6","doi-asserted-by":"publisher","first-page":"rbad068","DOI":"10.1093\/rb\/rbad068","type":"journal-article","article-title":"A controllable gelatin-based microcarriers fabrication system for the whole procedures of MSCs amplification and tissue engineering","volume":"10","author":"Wang","year":"2023","journal-title":"Regen. Biomater."},{"key":"bmmaeb214bib7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.137524","type":"journal-article","article-title":"Gelatin microcarriers as an effective adipose-derived stem cells delivery strategy in osteoarthritis treatment","volume":"283","author":"Peng","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"bmmaeb214bib8","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1002\/term.73","type":"journal-article","article-title":"Expansion of mesenchymal stem cells using a microcarrier-based cultivation system: growth and metabolism","volume":"2","author":"Schop","year":"2008","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"bmmaeb214bib9","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1016\/j.jcyt.2016.11.009","type":"journal-article","article-title":"Biodegradable poly-\u03f5-caprolactone microcarriers for efficient production of human mesenchymal stromal cells and secreted cytokines in batch and fed-batch bioreactors","volume":"19","author":"Lam","year":"2017","journal-title":"Cytotherapy"},{"key":"bmmaeb214bib10","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1002\/bit.22850","type":"journal-article","article-title":"Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells","volume":"107","author":"Storm","year":"2010","journal-title":"Biotechnol. Bioeng."},{"key":"bmmaeb214bib11","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.msec.2016.03.089","type":"journal-article","article-title":"Alginate\/PEG based microcarriers with cleavable crosslinkage for expansion and non-invasive harvest of human umbilical cord blood mesenchymal stem cells","volume":"64","author":"Li","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"bmmaeb214bib12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2022.213008","type":"journal-article","article-title":"Thermo\/redox-responsive dissolvable gelatin-based microsphere for efficient cell harvesting during 3D cell culturing","volume":"139","author":"Darge","year":"2022","journal-title":"Biomater. Adv."},{"key":"bmmaeb214bib13","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.bej.2014.02.005","type":"journal-article","article-title":"A potentially scalable method for the harvesting of hMSCs from microcarriers","volume":"85","author":"Nienow","year":"2014","journal-title":"Biochem. Eng. J."},{"key":"bmmaeb214bib14","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1002\/jbm.b.31394","type":"journal-article","article-title":"RGD modified PLGA\/gelatin microspheres as microcarriers for chondrocyte delivery","volume":"91B","author":"Tan","year":"2009","journal-title":"J. Biomed. Mater. Res. B"},{"key":"bmmaeb214bib15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.135176","type":"journal-article","article-title":"Development of injectable microgel-based scaffolds via enzymatic cross-linking of hyaluronic acid-tyramine\/gelatin-tyramine for potential bone tissue engineering","volume":"279","author":"Moghaddam","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"bmmaeb214bib16","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/adcc58","type":"journal-article","article-title":"Injectable microgel and micro-granular hydrogels for bone tissue engineering","volume":"17","author":"Mansouri Moghaddam","year":"2025","journal-title":"Biofabrication"},{"key":"bmmaeb214bib17","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2026.103103","type":"journal-article","article-title":"Compositional tuning of tyramine-modified gelatin-alginate microgels for modular tissue engineering applications","volume":"48","author":"Ghofrani-Beiragh","year":"2026","journal-title":"Appl. Mater. Today"},{"key":"bmmaeb214bib18","doi-asserted-by":"publisher","first-page":"4205","DOI":"10.1016\/j.biomaterials.2011.02.029","type":"journal-article","article-title":"Advantages of RGD peptides for directing cell association with biomaterials","volume":"32","author":"Bellis","year":"2011","journal-title":"Biomaterials"},{"key":"bmmaeb214bib19","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.112576","type":"journal-article","article-title":"3D bioprinting and photocrosslinking: emerging strategies & future perspectives","volume":"134","author":"Zennifer","year":"2022","journal-title":"Biomater. Adv."},{"key":"bmmaeb214bib20","article-title":"Novel Polymer.,WO2023097372","author":"Choong","year":"2022","type":"web-resource"},{"key":"bmmaeb214bib21","doi-asserted-by":"publisher","first-page":"4335","DOI":"10.3390\/molecules25184335","type":"journal-article","article-title":"Isolation and FTIR-ATR and 1H NMR characterization of alginates from the main alginophyte species of the atlantic coast of Morocco","volume":"25","author":"Belattmania","year":"2020","journal-title":"Molecules"},{"key":"bmmaeb214bib22","doi-asserted-by":"publisher","first-page":"8060","DOI":"10.1039\/C7TB01458A","type":"journal-article","article-title":"The development of cell-initiated degradable hydrogel based on methacrylated alginate applicable to multiple microfabrication technologies","volume":"5","author":"Sun","year":"2017","journal-title":"J. Mater. Chem. B"},{"key":"bmmaeb214bib23","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49244-4","type":"journal-article","article-title":"Centrifugal force-driven modular micronozzle system: generation of engineered alginate microspheres","volume":"9","author":"Kang","year":"2019","journal-title":"Sci. Rep."},{"key":"bmmaeb214bib24","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s13770-022-00487-9","type":"journal-article","article-title":"Towards clinical translation of in situ cartilage engineering strategies: optimizing the critical facets of a cell-laden hydrogel therapy","volume":"20","author":"Duchi","year":"2023","journal-title":"Tissue Eng. Regen. Med."},{"key":"bmmaeb214bib25","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2021.101150","type":"journal-article","article-title":"Synthesis, properties, and biomedical applications of alginate methacrylate (ALMA)-based hydrogels: current advances and challenges","volume":"24","author":"Hasany","year":"2021","journal-title":"Appl. Mater. Today"},{"key":"bmmaeb214bib26","doi-asserted-by":"publisher","first-page":"557","DOI":"10.3390\/md17100557","type":"journal-article","article-title":"Dual crosslinked methacrylated alginate hydrogel micron fibers and tissue constructs for cell biology","volume":"17","author":"Gao","year":"2019","journal-title":"Mar. Drugs"},{"key":"bmmaeb214bib27","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1177\/08853282231184294","type":"journal-article","article-title":"Architecting oxidized alginate methacrylate hydrogels with tunable characteristics by altering the sequence of the cross-linking steps, methacrylation reaction time, and polymer concentration","volume":"38","author":"Jalali Kandeloos","year":"2023","journal-title":"J. Biomater. Appl."},{"key":"bmmaeb214bib28","doi-asserted-by":"publisher","first-page":"2142","DOI":"10.1039\/C7SM02187A","type":"journal-article","article-title":"Tailoring the mechanical properties of gelatin methacryloyl hydrogels through manipulation of the photocrosslinking conditions","volume":"14","author":"O\u2019Connell","year":"2018","journal-title":"Soft Matter"},{"key":"bmmaeb214bib29","doi-asserted-by":"publisher","first-page":"264","DOI":"10.3390\/md19050264","type":"journal-article","article-title":"Preparation of alginate-based biomaterials and their applications in biomedicine","volume":"19","author":"Zhang","year":"2021","journal-title":"Mar. Drugs"},{"key":"bmmaeb214bib30","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.progpolymsci.2011.06.003","type":"journal-article","article-title":"Alginate: properties and biomedical applications","volume":"37","author":"Lee","year":"2012","journal-title":"Prog. Polym. Sci."},{"key":"bmmaeb214bib31","doi-asserted-by":"publisher","first-page":"85","DOI":"10.3390\/md19020085","type":"journal-article","article-title":"Influences of molecular weights on physicochemical and biological properties of collagen-alginate scaffolds","volume":"19","author":"Ho","year":"2021","journal-title":"Mar. Drugs"},{"key":"bmmaeb214bib32","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1016\/j.jmbbm.2016.02.003","type":"journal-article","article-title":"Mechanical properties and failure analysis of visible light crosslinked alginate-based tissue sealants","volume":"59","author":"Charron","year":"2016","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"bmmaeb214bib33","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121969","type":"journal-article","article-title":"Development and systematic characterization of GelMA\/alginate\/PEGDMA\/xanthan gum hydrogel bioink system for extrusion bioprinting","volume":"293","author":"Li","year":"2023","journal-title":"Biomaterials"},{"key":"bmmaeb214bib34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.129276","type":"journal-article","article-title":"Conductive GelMA\/alginate\/polypyrrole\/graphene hydrogel as a potential scaffold for cardiac tissue engineering; Physiochemical, mechanical, and biological evaluations","volume":"259","author":"Kaviani","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"bmmaeb214bib35","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2021.107947","type":"journal-article","article-title":"Agitation in a microcarrier-based spinner flask bioreactor modulates homeostasis of human mesenchymal stem cells","volume":"168","author":"Jeske","year":"2021","journal-title":"Biochem. Eng. J."},{"key":"bmmaeb214bib36","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1002\/elsc.201300013","type":"journal-article","article-title":"Stem cell engineering in bioreactors for large-scale bioprocessing","volume":"14","author":"Liu","year":"2014","journal-title":"Eng. Life Sci."},{"key":"bmmaeb214bib37","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1007\/s10529-023-03367-x","type":"journal-article","article-title":"Adipose-derived stem cells (ASCs) culture in spinner flask: improving the parameters of culture in a microcarrier-based system","volume":"45","author":"Sim\u00e3o","year":"2023","journal-title":"Biotechnol. Lett."},{"key":"bmmaeb214bib38","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1002\/term.545","type":"journal-article","article-title":"Modulation of mesenchymal stem cell actin organization on conventional microcarriers for proliferation and differentiation in stirred bioreactors","volume":"7","author":"Sart","year":"2013","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"bmmaeb214bib39","doi-asserted-by":"publisher","first-page":"829","DOI":"10.3390\/gels8120829","type":"journal-article","article-title":"Current advances in 3D dynamic cell culture systems","volume":"8","author":"Huang","year":"2022","journal-title":"Gels"},{"key":"bmmaeb214bib40","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2019.110264","type":"journal-article","article-title":"Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance","volume":"107","author":"Fontoura","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"bmmaeb214bib41","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1007\/s10616-017-0139-7","type":"journal-article","article-title":"Comparative studies of cellular viability levels on 2D and 3D in vitro culture matrices","volume":"70","author":"Gargotti","year":"2018","journal-title":"Cytotechnology"},{"key":"bmmaeb214bib42","doi-asserted-by":"publisher","first-page":"1340","DOI":"10.1002\/adma.201102560","type":"journal-article","article-title":"Controlled synthesis of 3D multi-compartmental particles with centrifuge-based microdroplet formation from a multi-barrelled capillary","volume":"24","author":"Maeda","year":"2012","journal-title":"Adv. Mater."},{"key":"bmmaeb214bib43","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1080\/00222348.2025.2488169","type":"journal-article","article-title":"Preparation and properties of hyaluronic acid\/glycidyl methacrylate microcarriers","volume":"65","author":"Lu","year":"2025","journal-title":"J. Macromol. Sci. B"},{"key":"bmmaeb214bib44","doi-asserted-by":"publisher","first-page":"2020","DOI":"10.3389\/fphar.2020.00654","type":"journal-article","article-title":"Biological considerations in scaling Up therapeutic cell manufacturing","volume":"11","author":"Cherian","year":"2020","journal-title":"Front. Pharmacol."},{"key":"bmmaeb214bib45","first-page":"77","type":"book","article-title":"Chapter 4\u2014Microcarrier culture systems for stem cell manufacturing","author":"Badenes","year":"2016"},{"key":"bmmaeb214bib46","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2023.100662","type":"journal-article","article-title":"A scalable culture system incorporating microcarrier for specialised mesenchymal stem cells from human embryonic stem cells","volume":"20","author":"Gao","year":"2023","journal-title":"Mater. Today Bio"},{"key":"bmmaeb214bib47","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.actbio.2015.10.046","type":"journal-article","article-title":"Cell sheets prepared via gel\u2013sol transition of calcium RGD\u2013alginate","volume":"30","author":"Yan","year":"2016","journal-title":"Acta Biomater."},{"key":"bmmaeb214bib48","doi-asserted-by":"publisher","first-page":"21641","DOI":"10.1021\/acsomega.0c02385","type":"journal-article","article-title":"Sacrificial alginate-assisted microfluidic engineering of cell-supportive protein microfibers for hydrogel-based cell encapsulation","volume":"5","author":"Saeki","year":"2020","journal-title":"ACS Omega"},{"key":"bmmaeb214bib49","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202100319","type":"journal-article","article-title":"Application of temporary, cell-containing alginate microcarriers to facilitate the fabrication of spatially defined cell pockets in 3D collagen hydrogels","volume":"22","author":"Lehnert","year":"2022","journal-title":"Macromol. Biosci."},{"key":"bmmaeb214bib50","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/j.ijbiomac.2020.02.042","type":"journal-article","article-title":"Multifunctional alginate-based hydrogel with reversible crosslinking for controlled therapeutics delivery","volume":"150","author":"Batool","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"bmmaeb214bib51","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1016\/j.tibtech.2024.01.001","type":"journal-article","article-title":"Mapping the microcarrier design pathway to modernise clinical mesenchymal stromal cell expansion","volume":"42","author":"Major","year":"2024","journal-title":"Trends Biotechnol."},{"key":"bmmaeb214bib52","doi-asserted-by":"publisher","first-page":"3491","DOI":"10.1021\/acsbiomaterials.0c00149","type":"journal-article","article-title":"Role of N-cadherin in a niche-mimicking microenvironment for chondrogenesis of mesenchymal stem cells in vitro","volume":"6","author":"Wang","year":"2020","journal-title":"ACS Biomater. Sci. Eng."},{"key":"bmmaeb214bib53","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2020.109297","type":"journal-article","article-title":"Effect of altering photocrosslinking conditions on the physical properties of alginate gels and the survival of photoencapsulated cells","volume":"179","author":"Zhao","year":"2020","journal-title":"Polym. Degrad. Stab."},{"key":"bmmaeb214bib54","doi-asserted-by":"publisher","first-page":"5837","DOI":"10.1038\/s41598-017-05699-x","type":"journal-article","article-title":"Handheld Co-axial bioprinting: application to in situ surgical cartilage repair","volume":"7","author":"Duchi","year":"2017","journal-title":"Sci. Rep."}],"container-title":["Biomedical Materials"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:10:22Z","timestamp":1792476622000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1748-605X\/aeb214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"references-count":54,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,10,20]]},"published-print":{"date-parts":[[2026,10,1]]}},"URL":"https:\/\/doi.org\/10.1088\/1748-605x\/aeb214","relation":{},"ISSN":["1748-6041","1748-605X"],"issn-type":[{"value":"1748-6041","type":"print"},{"value":"1748-605X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,1]]},"assertion":[{"value":"Alginate\u2013methacrylate\u2013RGD microspheres (Alg\u2013MR\u2013MS) for cell expansion and delivery in tissue engineering","name":"article_title","label":"Article Title"},{"value":"Biomedical Materials","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2026 The Author(s). Published by IOP Publishing Ltd","name":"copyright_information","label":"Copyright Information"},{"value":"2026-07-22","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-10-08","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-10-20","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}