{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T01:01:45Z","timestamp":1793581305414,"version":"4.5.0"},"reference-count":35,"publisher":"IOP Publishing","issue":"2","license":[{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"National High Technology Research and Development Program of China","award":["2012AA020506"],"award-info":[{"award-number":["2012AA020506"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31070860"],"award-info":[{"award-number":["31070860"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biofabrication"],"published-print":{"date-parts":[[2017,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Integrative osteochondral repair is a useful strategy for cartilage-defect repair. To mimic the microenvironment, it is necessary that scaffolds effectively mimic the extracellular matrix of natural cartilage and subchondral bone. In this study, biomimetic osteochondral scaffolds containing an oriented cartilage layer, a compact layer, and a three-dimensional (3D)-printed core-sheath structured-bone layer were developed. The oriented cartilage layer was designed to mimic the structural and material characteristics of native cartilage tissue and was fabricated with cartilage matrix-chitosan materials, using thermal-induced phase-separation technology. The 3D-printed core-sheath structured-bone layer was fabricated with poly(L-lactide-co-glycolide)\/\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -tricalcium phosphate-collagen materials by low-temperature deposition technology, using a specially designed core-sheath nozzle, and was designed to mimic the mechanical characteristics of subchondral bone and improve scaffold hydrophilicity. The compact layer was designed to mimic the calcified-layer structure of natural cartilage to ensure the presence of different suitable microenvironments for the regeneration of bone and cartilage. A dissolving-bonding process was developed to effectively combine the three parts together, after which the bone and cartilage scaffolds exhibited good mechanical properties and hydrophilicity. Additionally, goat autologous bone mesenchymal stem cells (BMSCs) were isolated and then seeded into the bone and cartilage layers, respectively, and following a 1 week culture\n                    <jats:italic>in vitro<\/jats:italic>\n                    , the BMSC-scaffold constructs were implanted into a goat articular-defect model. Our results indicated that the scaffolds exhibited good biocompatibility, and 24 weeks after implantation, the femoral condyle surface was relatively flat and consisted of a large quantity of hyaloid cartilage. Furthermore, histological staining revealed regenerated trabecular bone formed in the subchondral bone-defect area. These results provided a new method to fabricate biomimetic osteochondral scaffolds and demonstrated their effectiveness for future clinical applications in cartilage-defect repair.\n                  <\/jats:p>","DOI":"10.1088\/1758-5090\/aa7078","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T07:15:13Z","timestamp":1493709313000},"page":"025021","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":80,"title":["Biomimetic design and fabrication of multilayered osteochondral scaffolds by low-temperature deposition manufacturing and thermal-induced phase-separation techniques"],"prefix":"10.1088","volume":"9","author":[{"given":"Ting","family":"Zhang","sequence":"first","affiliation":[{"name":"Tsinghua University"},{"name":"Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084,"},{"name":"\u2018Biomanufacturing and Engineering Living Systems\u2019 Innovation International Talents Base (111 Base), Beijing, 100084,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hefeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tsinghua University"},{"name":"Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084,"},{"name":"\u2018Biomanufacturing and Engineering Living Systems\u2019 Innovation International Talents Base (111 Base), Beijing, 100084,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tsinghua University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaijun","family":"Jia","sequence":"additional","affiliation":[{"name":"Xi\u2019an Jiaotong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Liu","sequence":"additional","affiliation":[{"name":"The Fourth Military Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Xiong","sequence":"additional","affiliation":[{"name":"Tsinghua University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Sun","sequence":"additional","affiliation":[{"name":"Tsinghua University"},{"name":"Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084,"},{"name":"\u2018Biomanufacturing and Engineering Living Systems\u2019 Innovation International Talents Base (111 Base), Beijing, 100084,"},{"name":"Drexel University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2017,5,23]]},"reference":[{"key":"bfaa7078bib1","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1097\/JSA.0b013e31818cdb82","type":"journal-article","article-title":"Overview of existing cartilage repair technology","volume":"16","author":"Mcnickle","year":"2008","journal-title":"Sports Med. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2017-02-01","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2017-05-02","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2017-05-23","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}