{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:48:20Z","timestamp":1790884100860,"version":"4.1.0"},"reference-count":64,"publisher":"IOP Publishing","issue":"4","license":[{"start":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T00:00:00Z","timestamp":1532649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T00:00:00Z","timestamp":1532649600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"Faculty of Medicine of Technische Universit\u00e4t Dresden","award":["MeDDrive start grant"],"award-info":[{"award-number":["MeDDrive start grant"]}]},{"name":"German Federal Ministry for Economic Affairs and Energy","award":["KF3359301AK4"],"award-info":[{"award-number":["KF3359301AK4"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biofabrication"],"published-print":{"date-parts":[[2018,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix. In contrast, 3D bioprinting of soft cell-laden hydrogels (bioinks) enables the fabrication of constructs with spatially defined cell distribution, which has the potential to overcome problems of conventional cell seeding techniques\u2014but such objects lack mechanical stability. Herein, we combine 3D plotting of CPC and bioprinting of a cell-laden bioink for the first time. As model bioink, an alginate-methylcellulose blend (alg\/mc) was used, previously developed by us. Firstly, a fabrication regime was established, enabling optimal setting of CPC and cell survival inside the bioink. As the cells are exposed to the chemical changes of CPC precursors during setting, we studied the compatibility of the complex system of CPC and cell-laden alg\/mc for a combined extrusion process and characterized the cellular behavior of encapsulated human mesenchymal stroma cells within the bioink at the interface and in direct vicinity to the CPC. Furthermore, biphasic scaffolds were mechanically characterized and a model for osteochondral tissue grafts is proposed. The manuscript discusses possible impacts of the CPC setting reaction on cells within the bioink and illustrates the advantages of CPC in bioprinting as alternative to the commonly used thermoplasts for bone tissue engineering.<\/jats:p>","DOI":"10.1088\/1758-5090\/aad36d","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T13:43:39Z","timestamp":1531489419000},"page":"045002","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":92,"title":["Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink"],"prefix":"10.1088","volume":"10","author":[{"given":"Tilman","family":"Ahlfeld","sequence":"first","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falko","family":"Doberenz","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Kilian","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corina","family":"Vater","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Korn","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"},{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter","family":"Lauer","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7704-6435","authenticated-orcid":false,"given":"Anja","family":"Lode","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-5121","authenticated-orcid":false,"given":"Michael","family":"Gelinsky","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Dresden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2018,7,27]]},"reference":[{"key":"bfaad36dbib1","doi-asserted-by":"publisher","first-page":"3107","DOI":"10.1023\/A:1016189724389","type":"journal-article","article-title":"Fabrication of soft tissue engineering scaffolds by means of rapid prototyping techniques","volume":"37","author":"Landers","year":"2002","journal-title":"J. Mater. Sci."},{"key":"bfaad36dbib2","doi-asserted-by":"publisher","first-page":"5011","DOI":"10.1002\/adma.201302042","type":"journal-article","article-title":"25th anniversary article: engineering hydrogels for biofabrication","volume":"25","author":"Malda","year":"2013","journal-title":"Adv. Mater."},{"key":"bfaad36dbib3","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.biomaterials.2013.09.078","type":"journal-article","article-title":"The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability","volume":"35","author":"Billiet","year":"2014","journal-title":"Biomaterials"},{"key":"bfaad36dbib4","doi-asserted-by":"publisher","first-page":"7406","DOI":"10.1002\/adfm.201503423","type":"journal-article","article-title":"Bioprinting complex cartilaginous structures with clinically compliant biomaterials","volume":"25","author":"Kesti","year":"2015","journal-title":"Adv. Funct. Mater."},{"key":"bfaad36dbib5","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.ijbiomac.2017.10.105","type":"journal-article","article-title":"Develoupment of a tannic acid cross-linking process for obtaining 3D porous cell-laden collagen structure","volume":"110","author":"Lee","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"bfaad36dbib6","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1021\/acs.biomac.5b00188","type":"journal-article","article-title":"3D bioprinting human chondrocytes with nanocellulose-alginate bioink for cartilage tissue engineering applications","volume":"16","author":"Markstedt","year":"2015","journal-title":"Biomacromolecules"},{"key":"bfaad36dbib7","doi-asserted-by":"publisher","first-page":"2282","DOI":"10.1039\/c3tb21280g","type":"journal-article","article-title":"Development and characterisation of a new bioink for additive tissue manufacturing","volume":"2","author":"Melchels","year":"2014","journal-title":"J. Mater. Chem. B"},{"key":"bfaad36dbib8","doi-asserted-by":"publisher","first-page":"2816","DOI":"10.1002\/anie.201409846","type":"journal-article","article-title":"Biofabrication of cell-loaded 3D spider silk constructs","volume":"54","author":"Schacht","year":"2015","journal-title":"Angew. Chem., Int. Ed. Engl."},{"key":"bfaad36dbib9","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5082\/3\/2\/021001","type":"journal-article","article-title":"Bioprinting of hybrid tissue constructs with tailorable mechanical properties","volume":"3","author":"Schuurman","year":"2011","journal-title":"Biofabrication"},{"key":"bfaad36dbib10","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1002\/term.1682","type":"journal-article","article-title":"An additive manufacturing\u2010based PCL\u2013alginate\u2013chondrocyte bioprinted scaffold for cartilage tissue engineering","volume":"9","author":"Kundu","year":"2015","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"bfaad36dbib11","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5082\/6\/2\/024103","type":"journal-article","article-title":"3D printing of composite tissue with complex shape applied to ear regeneration","volume":"6","author":"Lee","year":"2014","journal-title":"Biofabrication"},{"key":"bfaad36dbib12","doi-asserted-by":"publisher","first-page":"3413","DOI":"10.1038\/nbt.3413","type":"journal-article","article-title":"A 3D bioprinting system to produce human-scale tissue constructs with structural integrity","volume":"34","author":"Kang","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"bfaad36dbib13","doi-asserted-by":"publisher","first-page":"3935","DOI":"10.1038\/ncomms4935","type":"journal-article","article-title":"Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink","volume":"5","author":"Pati","year":"2014","journal-title":"Nat. Commun."},{"key":"bfaad36dbib14","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/aa8cb7","type":"journal-article","article-title":"Double printing of hyaluronic acid\/poly(glycidol) hybrid hydrogels with poly(\u03b5-caprolactone) for MSC chondrogenesis","volume":"9","author":"Stichler","year":"2017","journal-title":"Biofabrication"},{"key":"bfaad36dbib15","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1089\/ten.tea.2016.0498","type":"journal-article","article-title":"Three-dimensional bioprinting of polycaprolactone reinforced gene activated bioinks for bone tissue engineering","volume":"23","author":"Cunniffe","year":"2017","journal-title":"Tissue Eng. A"},{"key":"bfaad36dbib16","doi-asserted-by":"publisher","first-page":"2353","DOI":"10.1002\/adhm.201600182","type":"journal-article","article-title":"3D bioprinting of developmentally inspired templates for whole bone organ engineering","volume":"5","author":"Daly","year":"2016","journal-title":"Adv. Healthc. Mater."},{"key":"bfaad36dbib17","doi-asserted-by":"publisher","DOI":"10.1088\/0960-1317\/22\/8\/085014","type":"journal-article","article-title":"Bioprinting of a mechanically enhanced three-dimensional dual cell-laden construct for osteochondral tissue engineering using a multi-head tissue\/organ building system","volume":"22","author":"Shim","year":"2012","journal-title":"J. Micromech. Microeng."},{"key":"bfaad36dbib18","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/1\/014102","type":"journal-article","article-title":"Three-dimensional bioprinting of multilayered constructs containing human mesenchymal stromal cells for osteochondral tissue regeneration in the rabbit knee joint","volume":"8","author":"Shim","year":"2016","journal-title":"Biofabrication"},{"key":"bfaad36dbib19","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/3\/035013","type":"journal-article","article-title":"Three-dimensional bioprinting of cell-laden constructs with polycaprolactone protective layers for using various thermoplastic polymers","volume":"8","author":"Kim","year":"2016","journal-title":"Biofabrication"},{"key":"bfaad36dbib20","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.msec.2016.11.049","type":"journal-article","article-title":"Comparison between PCL\/hydroxyapatite (HA) and PCL\/halloysite nanotube (HNT) composite scaffolds prepared by co-extrusion and gas foaming","volume":"72","author":"Jing","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"bfaad36dbib21","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.3390\/ma10101123","type":"journal-article","article-title":"Production of poly(\u03b5-caprolactone)\/hydroxyapatite composite scaffolds with a tailored macro\/micro-porous structure, high mechanical properties, and excellent bioactivity","volume":"10","author":"Kim","year":"2017","journal-title":"Materials"},{"key":"bfaad36dbib22","doi-asserted-by":"publisher","DOI":"10.1002\/app.43031","type":"journal-article","article-title":"Fabrication of PCL\/\u03b2-TCP scaffolds by 3D mini-screw extrusion printing","volume":"133","author":"D\u00e1vila","year":"2016","journal-title":"J. Appl. Polym. Sci."},{"key":"bfaad36dbib23","doi-asserted-by":"publisher","first-page":"5369","DOI":"10.1016\/j.actbio.2012.10.009","type":"journal-article","article-title":"Moisture based three-dimensional printing of calcium phosphate structures for scaffold engineering","volume":"9","author":"Butscher","year":"2013","journal-title":"Acta Biomater."},{"key":"bfaad36dbib24","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5082\/6\/1\/015006","type":"journal-article","article-title":"Direct 3D powder printing of biphasic calcium phosphate scaffolds for substitution of complex bone defects","volume":"6","author":"Castilho","year":"2014","journal-title":"Biofabrication"},{"key":"bfaad36dbib25","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.actbio.2006.02.004","type":"journal-article","article-title":"Sintering and robocasting of \u03b2-tricalcium phosphate scaffolds for orthopaedic applications","volume":"2","author":"Miranda","year":"2006","journal-title":"Acta Biomater."},{"key":"bfaad36dbib26","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1016\/j.actbio.2009.06.031","type":"journal-article","article-title":"Direct write assembly of calcium phosphate scaffolds using a water-based hydrogel","volume":"6","author":"Franco","year":"2010","journal-title":"Acta Biomater."},{"key":"bfaad36dbib27","doi-asserted-by":"publisher","first-page":"3119","DOI":"10.1007\/s10856-010-4166-6","type":"journal-article","article-title":"Indirect rapid prototyping of biphasic calcium phosphate scaffolds as bone substitutes: influence of phase composition, macroporosity and pore geometry on mechanical properties","volume":"21","author":"Schumacher","year":"2010","journal-title":"J. Mater. Sci., Mater. Med."},{"key":"bfaad36dbib28","doi-asserted-by":"publisher","first-page":"5378","DOI":"10.1039\/C4TB00438H","type":"journal-article","article-title":"Robocasting of biomimetic hydroxyapatite scaffolds using self-setting inks","volume":"2","author":"Maazouz","year":"2014","journal-title":"J. Mater. Chem. B"},{"key":"bfaad36dbib29","doi-asserted-by":"publisher","first-page":"41722","DOI":"10.1021\/acsami.7b14175","type":"journal-article","article-title":"Osteoinduction by foamed and 3D-printed calcium phosphate scaffolds: effect of nanostructure and pore architecture","volume":"9","author":"Barba","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"bfaad36dbib30","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1016\/j.actbio.2016.11.043","type":"journal-article","article-title":"Self-hardening and thermoresponsive alpha tricalcium phosphate\/pluronic pastes","volume":"49","author":"Maazouz","year":"2017","journal-title":"Acta Biomater."},{"key":"bfaad36dbib31","doi-asserted-by":"publisher","first-page":"6199","DOI":"10.1016\/j.actbio.2012.12.017","type":"journal-article","article-title":"Properties of injectable ready-to-use calcium phosphate cement based on water-immiscible liquid","volume":"9","author":"Heinemann","year":"2013","journal-title":"Acta Biomater."},{"key":"bfaad36dbib32","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1002\/term.1563","type":"journal-article","article-title":"Fabrication of porous scaffolds by three-dimensional plotting of a pasty calcium phosphate bone cement under mild conditions","volume":"8","author":"Lode","year":"2014","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"bfaad36dbib33","doi-asserted-by":"publisher","first-page":"4088","DOI":"10.1039\/c3tb20511h","type":"journal-article","article-title":"Well-ordered biphasic calcium phosphate\u2013alginate scaffolds fabricated by multi-channel 3D plotting under mild conditions","volume":"1","author":"Luo","year":"2013","journal-title":"J. Mater. Chem. B"},{"key":"bfaad36dbib34","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.actbio.2015.08.036","type":"journal-article","article-title":"3D plotting of growth factor loaded calcium phosphate cement scaffolds","volume":"27","author":"Akkineni","year":"2015","journal-title":"Acta Biomater."},{"key":"bfaad36dbib35","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1007\/s10439-016-1685-4","type":"journal-article","article-title":"Design and fabrication of complex scaffolds for bone defect healing: combined 3D plotting of a calcium phosphate cement and a growth factor-loaded hydrogel","volume":"45","author":"Ahlfeld","year":"2017","journal-title":"Ann. Biomed. Eng."},{"key":"bfaad36dbib36","doi-asserted-by":"publisher","first-page":"1574","DOI":"10.1002\/term.2058","type":"journal-article","article-title":"Three-dimensional plotting of a cell-laden alginate\/methylcellulose blend: towards biofabrication of tissue engineering constructs with clinically relevant dimensions","volume":"11","author":"Sch\u00fctz","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"bfaad36dbib37","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1002\/jbm.b.30082","type":"journal-article","article-title":"Proliferation and differentiation of osteoblasts on biocement D modified with collagen type I and citric acid","volume":"71B","author":"Hempel","year":"2004","journal-title":"J. Biomed. Mater. Res. B"},{"key":"bfaad36dbib38","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1111\/j.1582-4934.2008.00299.x","type":"journal-article","article-title":"Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer","volume":"12","author":"B\u00f6cker","year":"2008","journal-title":"J. Cell. Mol. Med."},{"key":"bfaad36dbib39","doi-asserted-by":"publisher","first-page":"7536","DOI":"10.1016\/j.actbio.2013.03.014","type":"journal-article","article-title":"A novel and easy-to-prepare strontium(II) modified calcium phosphate bone cement with enhanced mechanical properties","volume":"9","author":"Schumacher","year":"2013","journal-title":"Acta Biomater."},{"key":"bfaad36dbib40","doi-asserted-by":"publisher","first-page":"23","DOI":"10.3389\/fbioe.2017.00023","type":"journal-article","article-title":"Recent advances in bioink design for 3D bioprinting of tissues and organs","volume":"5","author":"Ji","year":"2017","journal-title":"Front. Bioeng. Biotechnol."},{"key":"bfaad36dbib41","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1002\/1097-4636(200105)55:2<203::AID-JBM1007>3.0.CO;2-7","type":"journal-article","article-title":"Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling","volume":"55","author":"Hutmacher","year":"2001","journal-title":"J. Biomed. Mater. Res."},{"key":"bfaad36dbib42","doi-asserted-by":"publisher","first-page":"4317","DOI":"10.1002\/jbm.a.35107","type":"journal-article","article-title":"Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds","volume":"102","author":"Temple","year":"2014","journal-title":"J. Biomed. Mater. Res. A"},{"key":"bfaad36dbib43","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/3\/035004","type":"journal-article","article-title":"Hydrogel-based reinforcement of 3D bioprinted constructs","volume":"8","author":"Melchels","year":"2016","journal-title":"Biofabrication"},{"key":"bfaad36dbib44","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1596157","type":"journal-article","article-title":"Fabrication of poly(\u03b5-caprolactone) scaffolds reinforced with cellulose nanofibers, with and without the addition of hydroxyapatite nanoparticles","volume":"2016","author":"Morou\u00e7o","year":"2016","journal-title":"BioMed Res. Int."},{"key":"bfaad36dbib45","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1021\/acs.langmuir.5b03754","type":"journal-article","article-title":"(9R)\u22129-hydroxystearate-functionalized hydroxyapatite as antiproliferative and cytotoxic agent toward osteosarcoma cells","volume":"32","author":"Boanini","year":"2016","journal-title":"Langmuir"},{"key":"bfaad36dbib46","doi-asserted-by":"publisher","first-page":"3557","DOI":"10.1016\/j.actbio.2010.03.022","type":"journal-article","article-title":"Nanostructured poly(\u03b5-caprolactone)\u2013silica xerogel fibrous membrane for guided bone regeneration","volume":"6","author":"Lee","year":"2010","journal-title":"Acta Biomater."},{"key":"bfaad36dbib47","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/321549","type":"journal-article","article-title":"The osteogenesis of bone marrow stem cells on mPEG-PCL-mPEG\/hydroxyapatite composite scaffold via solid freeform fabrication","volume":"2014","author":"Liao","year":"2014","journal-title":"BioMed Res. Int."},{"key":"bfaad36dbib48","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.biomaterials.2006.08.031","type":"journal-article","article-title":"The effect of adsorbed serum proteins, RGD and proteoglycan-binding peptides on the adhesion of mesenchymal stem cells to hydroxyapatite","volume":"28","author":"Sawyer","year":"2007","journal-title":"Biomaterials"},{"key":"bfaad36dbib49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016813","type":"journal-article","article-title":"Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite","volume":"6","author":"Phipps","year":"2011","journal-title":"PLoS One"},{"key":"bfaad36dbib50","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.actbio.2017.10.036","type":"journal-article","article-title":"Strontium-modified premixed calcium phosphate cements for the therapy of osteoporotic bone defects","volume":"65","author":"Lode","year":"2018","journal-title":"Acta Biomater."},{"key":"bfaad36dbib51","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1016\/j.addr.2012.01.008","type":"journal-article","article-title":"Calcium phosphate cements as drug delivery materials","volume":"64","author":"Ginebra","year":"2012","journal-title":"Adv. Drug Deliv. Rev."},{"key":"bfaad36dbib52","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1089\/ten.tec.2014.0187","type":"journal-article","article-title":"Formation of osteoclasts on calcium phosphate bone cements and polystyrene depends on monocyte isolation conditions","volume":"21","author":"Bernhardt","year":"2014","journal-title":"Tissue Eng. C"},{"key":"bfaad36dbib53","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1016\/j.clinbiochem.2012.03.021","type":"journal-article","article-title":"An overview of the regulation of bone remodelling at the cellular level","volume":"45","author":"Kular","year":"2012","journal-title":"Clin. Biochem."},{"key":"bfaad36dbib54","first-page":"301","type":"journal-article","article-title":"Materials and scaffolds in medical 3D printing and bioprinting in the context of bone regeneration","volume":"19","author":"Heller","year":"2016","journal-title":"Int. J. Comput. Dent."},{"key":"bfaad36dbib55","doi-asserted-by":"publisher","first-page":"19468","DOI":"10.1038\/srep19468","type":"journal-article","article-title":"Design and fabrication of 3D printed Scaffolds with a mechanical strength comparable to cortical bone to repair large bone defects","volume":"6","author":"Roohani-Esfahani","year":"2016","journal-title":"Sci. Rep."},{"key":"bfaad36dbib56","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1016\/j.jmbbm.2009.01.009","type":"journal-article","article-title":"The influence of micropore size on the mechanical properties of bulk hydroxyapatite and hydroxyapatite scaffolds","volume":"2","author":"Cordell","year":"2009","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"bfaad36dbib57","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/0022-0248(93)90265-X","type":"journal-article","article-title":"The low temperature formation of octacalcium phosphate","volume":"132","author":"Graham","year":"1993","journal-title":"J. Cryst. Growth"},{"key":"bfaad36dbib58","doi-asserted-by":"publisher","first-page":"4628","DOI":"10.1021\/cr0782574","type":"journal-article","article-title":"Calcium orthophosphates: crystallization and dissolution","volume":"108","author":"Wang","year":"2008","journal-title":"Chem. Rev."},{"key":"bfaad36dbib59","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1016\/j.msec.2017.02.158","type":"journal-article","article-title":"Engineering bone regeneration with novel cell-laden hydrogel microfiber-injectable calcium phosphate scaffold","volume":"75","author":"Song","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"bfaad36dbib60","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/978-1-4614-1080-5_2","type":"book","article-title":"Matrix stiffness: a regulator of cellular behavior and tissue formation","author":"Mason","year":"2012"},{"key":"bfaad36dbib61","doi-asserted-by":"publisher","DOI":"10.4172\/2155-952X.1000150","type":"journal-article","article-title":"Effects of surface roughness of hydroxyapatite on cell attachment and proliferation","volume":"2","author":"Li","year":"2012","journal-title":"J. Biotechnol. Biomater."},{"key":"bfaad36dbib62","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/4\/045001","type":"journal-article","article-title":"A versatile method for combining different biopolymers in a core\/shell fashion by 3D plotting to achieve mechanically robust constructs","volume":"8","author":"Akkineni","year":"2016","journal-title":"Biofabrication"},{"key":"bfaad36dbib63","doi-asserted-by":"publisher","first-page":"4707","DOI":"10.1039\/C6TB00849F","type":"journal-article","article-title":"A simultaneous 3D printing process for the fabrication of bioceramic and cell-laden hydrogel core\/shell scaffolds with potential application in bone tissue regeneration","volume":"4","author":"Raja","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"bfaad36dbib64","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/aa9ef1","type":"journal-article","article-title":"Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs","volume":"10","author":"Mekhileri","year":"2018","journal-title":"Biofabrication"}],"container-title":["Biofabrication"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d","content-type":"text\/html","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T12:24:07Z","timestamp":1779539047000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/aad36d"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,27]]},"references-count":64,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,7,27]]},"published-print":{"date-parts":[[2018,9,1]]}},"URL":"https:\/\/doi.org\/10.1088\/1758-5090\/aad36d","relation":{},"ISSN":["1758-5082","1758-5090"],"issn-type":[{"value":"1758-5082","type":"print"},{"value":"1758-5090","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,27]]},"assertion":[{"value":"Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink","name":"article_title","label":"Article Title"},{"value":"Biofabrication","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2018 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2018-03-20","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2018-07-13","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2018-07-27","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}