{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:13:59Z","timestamp":1787505239397,"version":"build-2736575974"},"reference-count":118,"publisher":"IOP Publishing","issue":"4","license":[{"start":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:00:00Z","timestamp":1692576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:00:00Z","timestamp":1692576000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32025007"],"award-info":[{"award-number":["32025007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Key Research and Development Program of China","award":["2018YFA0108100"],"award-info":[{"award-number":["2018YFA0108100"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biofabrication"],"published-print":{"date-parts":[[2023,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Organoid technology offers sophisticated\n                    <jats:italic>in vitro<\/jats:italic>\n                    human models for basic research and drug development. However, low batch-to-batch reproducibility and high cost due to laborious procedures and materials prevent organoid culture standardization for automation and high-throughput applications. Here, using a novel platform based on the findings that Pluronic F-127 (PF-127) could trigger highly uniform spheroid assembly through a mechanism different from plate coating, we develop a one-pot organoid differentiation strategy. Using our strategy, we successfully generate cortical, nephron, hepatic, and lung organoids with improved reproducibility compared to previous methods while reducing the original costs by 80%\u201395%. In addition, we adapt our platform to microfluidic chips allowing automated culture. We showcase that our platform can be applied to tissue-specific screening, such as drug toxicity and transfection reagents testing. Finally, we generate\n                    <jats:italic>NEAT1<\/jats:italic>\n                    knockout tissue-specific organoids and show\n                    <jats:italic>NEAT1<\/jats:italic>\n                    modulates multiple signaling pathways fine-tuning the differentiation of nephron and hepatic organoids and suppresses immune responses in cortical organoids. In summary, our strategy provides a powerful platform for advancing organoid research and studying human development and diseases.\n                  <\/jats:p>","DOI":"10.1088\/1758-5090\/acee21","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T18:27:28Z","timestamp":1691519248000},"page":"045014","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly"],"prefix":"10.1088","volume":"15","author":[{"given":"Xiao-Shan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5672-732X","authenticated-orcid":true,"given":"Gang","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangjin","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Xia","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Fei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5213-5084","authenticated-orcid":true,"given":"Qiang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7297-1266","authenticated-orcid":false,"given":"Yanyi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6974-6060","authenticated-orcid":true,"given":"Yangming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2023,8,21]]},"reference":[{"key":"bfacee21bib1","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1126\/science.282.5391.1145","article-title":"Embryonic stem cell lines derived from human blastocysts","volume":"282","author":"Thomson","year":"1998","journal-title":"Science"},{"key":"bfacee21bib2","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1016\/j.cell.2007.11.019","article-title":"Induction of pluripotent stem cells from adult human fibroblasts by defined factors","volume":"131","author":"Takahashi","year":"2007","journal-title":"Cell"},{"key":"bfacee21bib3","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1002\/jcp.24683","article-title":"3D cell culture systems: advantages and applications","volume":"230","author":"Ravi","year":"2015","journal-title":"J. Cell. Physiol."},{"key":"bfacee21bib4","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1073\/pnas.84.1.136","article-title":"Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells","volume":"84","author":"Li","year":"1987","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"bfacee21bib5","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.stem.2008.09.002","article-title":"Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals","volume":"3","author":"Eiraku","year":"2008","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib6","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1038\/nature07935","article-title":"Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche","volume":"459","author":"Sato","year":"2009","journal-title":"Nature"},{"key":"bfacee21bib7","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1038\/nature12517","article-title":"Cerebral organoids model human brain development and microcephaly","volume":"501","author":"Lancaster","year":"2013","journal-title":"Nature"},{"key":"bfacee21bib8","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1016\/j.cell.2016.04.032","article-title":"Brain-region-specific organoids using mini-bioreactors for modeling ZIKV exposure","volume":"165","author":"Qian","year":"2016","journal-title":"Cell"},{"key":"bfacee21bib9","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.3415","article-title":"Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture","volume":"12","author":"Pasca","year":"2015","journal-title":"Nat. Methods"},{"key":"bfacee21bib10","doi-asserted-by":"publisher","first-page":"15672","DOI":"10.1073\/pnas.1520760112","article-title":"Human cerebral organoids recapitulate gene expression programs of fetal neocortex development","volume":"112","author":"Camp","year":"2015","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"bfacee21bib11","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1038\/nmeth.4304","article-title":"Fused cerebral organoids model interactions between brain regions","volume":"14","author":"Bagley","year":"2017","journal-title":"Nat. Methods"},{"key":"bfacee21bib12","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1038\/nature22330","article-title":"Assembly of functionally integrated human forebrain spheroids","volume":"545","author":"Birey","year":"2017","journal-title":"Nature"},{"key":"bfacee21bib13","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.stem.2017.07.007","article-title":"Fusion of regionally specified hPSC-derived organoids models human brain development and interneuron migration","volume":"21","author":"Xiang","year":"2017","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib14","doi-asserted-by":"publisher","first-page":"1762","DOI":"10.1053\/j.gastro.2011.07.050","article-title":"Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett\u2019s epithelium","volume":"141","author":"Sato","year":"2011","journal-title":"Gastroenterology"},{"key":"bfacee21bib15","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1038\/nm.2470","article-title":"Isolation and in vitro expansion of human colonic stem cells","volume":"17","author":"Jung","year":"2011","journal-title":"Nat. Med."},{"key":"bfacee21bib16","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1038\/nature09691","article-title":"Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro","volume":"470","author":"Spence","year":"2011","journal-title":"Nature"},{"key":"bfacee21bib17","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1242\/dev.138453","article-title":"in vitro patterning of pluripotent stem cell-derived intestine recapitulates in vivo human development","volume":"144","author":"Tsai","year":"2017","journal-title":"Development"},{"key":"bfacee21bib18","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.stem.2009.11.013","article-title":"Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro","volume":"6","author":"Barker","year":"2010","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib19","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/j.cell.2013.09.008","article-title":"Differentiated Troy+ chief cells act as reserve stem cells to generate all lineages of the stomach epithelium","volume":"155","author":"Stange","year":"2013","journal-title":"Cell"},{"key":"bfacee21bib20","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1053\/j.gastro.2014.09.042","article-title":"in vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection","volume":"148","author":"Bartfeld","year":"2015","journal-title":"Gastroenterology"},{"key":"bfacee21bib21","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1038\/nature21021","article-title":"Wnt\/beta-catenin promotes gastric fundus specification in mice and humans","volume":"541","author":"McCracken","year":"2017","journal-title":"Nature"},{"key":"bfacee21bib22","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1038\/nature13863","article-title":"Modelling human development and disease in pluripotent stem-cell-derived gastric organoids","volume":"516","author":"McCracken","year":"2014","journal-title":"Nature"},{"key":"bfacee21bib23","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1038\/nature12271","article-title":"Vascularized and functional human liver from an iPSC-derived organ bud transplant","volume":"499","author":"Takebe","year":"2013","journal-title":"Nature"},{"key":"bfacee21bib24","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1038\/nbt.3294","article-title":"Directed differentiation of cholangiocytes from human pluripotent stem cells","volume":"33","author":"Ogawa","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"bfacee21bib25","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1038\/nbt.3275","article-title":"Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation","volume":"33","author":"Sampaziotis","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"bfacee21bib26","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1038\/nature22796","article-title":"Multilineage communication regulates human liver bud development from pluripotency","volume":"546","author":"Camp","year":"2017","journal-title":"Nature"},{"key":"bfacee21bib27","doi-asserted-by":"publisher","first-page":"1591","DOI":"10.1016\/j.cell.2018.11.013","article-title":"Long-term expansion of functional mouse and human hepatocytes as 3D organoids","volume":"175","author":"Hu","year":"2018","journal-title":"Cell"},{"key":"bfacee21bib28","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.1038\/s41422-019-0242-8","article-title":"Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury","volume":"29","author":"Wang","year":"2019","journal-title":"Cell Res."},{"key":"bfacee21bib29","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1038\/nature15695","article-title":"Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis","volume":"526","author":"Takasato","year":"2015","journal-title":"Nature"},{"key":"bfacee21bib30","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1038\/nbt.3392","article-title":"Nephron organoids derived from human pluripotent stem cells model kidney development and injury","volume":"33","author":"Morizane","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"bfacee21bib31","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.stem.2013.11.010","article-title":"Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells","volume":"14","author":"Taguchi","year":"2014","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib32","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1038\/ncb2894","article-title":"Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney","volume":"16","author":"Takasato","year":"2014","journal-title":"Nat. Cell Biol."},{"key":"bfacee21bib33","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1016\/j.stem.2017.10.011","article-title":"Higher-order kidney organogenesis from pluripotent stem cells","volume":"21","author":"Taguchi","year":"2017","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib34","doi-asserted-by":"publisher","first-page":"8715","DOI":"10.1038\/ncomms9715","article-title":"Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids","volume":"6","author":"Freedman","year":"2015","journal-title":"Nat. Commun."},{"key":"bfacee21bib35","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1038\/ncb3510","article-title":"A three-dimensional model of human lung development and disease from pluripotent stem cells","volume":"19","author":"Chen","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"bfacee21bib36","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1038\/s41596-018-0104-8","article-title":"Generation of lung organoids from human pluripotent stem cells in vitro","volume":"14","author":"Miller","year":"2019","journal-title":"Nat. Protocols"},{"key":"bfacee21bib37","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.19732","article-title":"A bioengineered niche promotes in vivo engraftment and maturation of pluripotent stem cell derived human lung organoids","volume":"5","author":"Dye","year":"2016","journal-title":"Elife"},{"key":"bfacee21bib38","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05098","article-title":"in vitro generation of human pluripotent stem cell derived lung organoids","volume":"4","author":"Dye","year":"2015","journal-title":"Elife"},{"key":"bfacee21bib39","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2018100300","article-title":"Long-term expanding human airway organoids for disease modeling","volume":"38","author":"Sachs","year":"2019","journal-title":"EMBO J."},{"key":"bfacee21bib40","doi-asserted-by":"publisher","first-page":"876","DOI":"10.1038\/nbt.2328","article-title":"Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein","volume":"30","author":"Wong","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"bfacee21bib41","doi-asserted-by":"publisher","first-page":"2612","DOI":"10.1038\/s41467-019-10497-2","article-title":"Modeling microcephaly with cerebral organoids reveals a WDR62-CEP170-KIF2A pathway promoting cilium disassembly in neural progenitors","volume":"10","author":"Zhang","year":"2019","journal-title":"Nat. Commun."},{"key":"bfacee21bib42","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1101\/gad.332494.119","article-title":"Cerebral organoid and mouse models reveal a RAB39b-PI3K-mTOR pathway-dependent dysregulation of cortical development leading to macrocephaly\/autism phenotypes","volume":"34","author":"Zhang","year":"2020","journal-title":"Genes Dev."},{"key":"bfacee21bib43","doi-asserted-by":"publisher","first-page":"941","DOI":"10.1038\/s41593-018-0175-4","article-title":"A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer\u2019s disease","volume":"21","author":"Park","year":"2018","journal-title":"Nat. Neurosci."},{"key":"bfacee21bib44","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1016\/j.stemcr.2019.01.020","article-title":"Modeling G2019S-LRRK2 sporadic Parkinson\u2019s disease in 3D midbrain organoids","volume":"12","author":"Kim","year":"2019","journal-title":"Stem Cell Rep."},{"key":"bfacee21bib45","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/j.stem.2017.06.017","article-title":"High-content screening in hPSC-neural progenitors identifies drug candidates that inhibit zika virus infection in fetal-like organoids and adult brain","volume":"21","author":"Zhou","year":"2017","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib46","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1016\/j.stem.2018.04.022","article-title":"High-throughput screening enhances kidney organoid differentiation from human pluripotent stem cells and enables automated multidimensional phenotyping","volume":"22","author":"Czerniecki","year":"2018","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib47","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1038\/nm.3201","article-title":"A functional CFTR assay using primary cystic fibrosis intestinal organoids","volume":"19","author":"Dekkers","year":"2013","journal-title":"Nat. Med."},{"key":"bfacee21bib48","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1016\/j.stem.2018.09.016","article-title":"A comprehensive human gastric cancer organoid biobank captures tumor subtype heterogeneity and enables therapeutic screening","volume":"23","author":"Yang","year":"2018","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib49","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/j.cell.2016.08.041","article-title":"A biobank of breast cancer explants with preserved intra-tumor heterogeneity to screen anticancer compounds","volume":"167","author":"Bruna","year":"2016","journal-title":"Cell"},{"key":"bfacee21bib50","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1126\/science.aao2774","article-title":"Patient-derived organoids model treatment response of metastatic gastrointestinal cancers","volume":"359","author":"Vlachogiannis","year":"2018","journal-title":"Science"},{"key":"bfacee21bib51","doi-asserted-by":"publisher","first-page":"344ra84","DOI":"10.1126\/scitranslmed.aad8278","article-title":"Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis","volume":"8","author":"Dekkers","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"bfacee21bib52","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.cell.2017.11.010","article-title":"A living biobank of breast cancer organoids captures disease heterogeneity","volume":"172","author":"Sachs","year":"2018","journal-title":"Cell"},{"key":"bfacee21bib53","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1038\/nature09941","article-title":"Self-organizing optic-cup morphogenesis in three-dimensional culture","volume":"472","author":"Eiraku","year":"2011","journal-title":"Nature"},{"key":"bfacee21bib54","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.cell.2019.01.017","article-title":"Establishing cerebral organoids as models of human-specific brain evolution","volume":"176","author":"Pollen","year":"2019","journal-title":"Cell"},{"key":"bfacee21bib55","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3389\/fmolb.2020.00033","article-title":"Is it time to start transitioning from 2D to 3D cell culture?","volume":"7","author":"Jensen","year":"2020","journal-title":"Front. Mol. Biosci."},{"key":"bfacee21bib56","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/s41578-020-0199-8","article-title":"Synthetic alternatives to Matrigel","volume":"5","author":"Aisenbrey","year":"2020","journal-title":"Nat. Rev. Mater."},{"key":"bfacee21bib57","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107814","article-title":"Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium","volume":"9","author":"Jabaji","year":"2014","journal-title":"PLoS One"},{"key":"bfacee21bib58","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1038\/ncb3632","article-title":"Synthetic hydrogels for human intestinal organoid generation and colonic wound repair","volume":"19","author":"Cruz-Acuna","year":"2017","journal-title":"Nat. Cell Biol."},{"key":"bfacee21bib59","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1038\/s42003-021-02910-8","article-title":"Towards organoid culture without Matrigel","volume":"4","author":"Kozlowski","year":"2021","journal-title":"Commun. Biol."},{"key":"bfacee21bib60","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1038\/s41578-021-00279-y","article-title":"Engineering organoids","volume":"6","author":"Hofer","year":"2021","journal-title":"Nat. Rev. Mater."},{"key":"bfacee21bib61","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1038\/s41378-020-00185-3","article-title":"Microtechnology-based methods for organoid models","volume":"6","author":"Velasco","year":"2020","journal-title":"Microsyst. Nanoeng."},{"key":"bfacee21bib62","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2022.100297","article-title":"Deep-learning analysis of micropattern-based organoids enables high-throughput drug screening of Huntington\u2019s disease models","volume":"2","author":"Metzger","year":"2022","journal-title":"Cell Rep. Methods"},{"key":"bfacee21bib63","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1038\/s41551-021-00757-2","article-title":"Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip","volume":"5","author":"Wiedenmann","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"bfacee21bib64","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1038\/s41551-020-0565-2","article-title":"High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays","volume":"4","author":"Brandenberg","year":"2020","journal-title":"Nat. Biomed. Eng."},{"key":"bfacee21bib65","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1038\/s41563-020-00853-9","article-title":"Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation","volume":"20","author":"Lawlor","year":"2021","journal-title":"Nat. Mater."},{"key":"bfacee21bib66","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1007\/s40846-015-0029-4","article-title":"Anti-fouling coatings of poly(dimethylsiloxane) devices for biological and biomedical applications","volume":"35","author":"Zhang","year":"2015","journal-title":"J. Med. Biol. Eng."},{"key":"bfacee21bib67","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.jconrel.2008.04.013","article-title":"Pluronic block copolymers: evolution of drug delivery concept from inert nanocarriers to biological response modifiers","volume":"130","author":"Batrakova","year":"2008","journal-title":"J. Control Release"},{"key":"bfacee21bib68","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1142\/S2339547817300013","article-title":"Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology","volume":"5","author":"Gokaltun","year":"2017","journal-title":"Technology"},{"key":"bfacee21bib69","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1039\/D2SD00004K","article-title":"Uniformity of spheroids-on-a-chip by surface treatment of PDMS microfluidic platforms","volume":"1","author":"Azizipour","year":"2022","journal-title":"Sens. Diagn."},{"key":"bfacee21bib70","doi-asserted-by":"publisher","first-page":"e27","DOI":"10.1038\/am.2012.50","article-title":"Configurable 2D and 3D spheroid tissue cultures on bioengineered surfaces with acquisition of epithelial-mesenchymal transition characteristics","volume":"4","author":"Kuo","year":"2012","journal-title":"NPG Asia Mater."},{"key":"bfacee21bib71","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/abb529","article-title":"Membrane-bottomed microwell array added to Transwell insert to facilitate non-contact co-culture of spermatogonial stem cell and STO feeder cell","volume":"12","author":"Lee","year":"2020","journal-title":"Biofabrication"},{"key":"bfacee21bib72","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/s41378-020-00201-6","article-title":"A microfluidic platform for cultivating ovarian cancer spheroids and testing their responses to chemotherapies","volume":"6","author":"Dadgar","year":"2020","journal-title":"Microsyst. Nanoeng."},{"key":"bfacee21bib73","doi-asserted-by":"publisher","first-page":"48478","DOI":"10.1021\/acsami.1c18249","article-title":"Impact of a desmoplastic tumor microenvironment for colon cancer drug sensitivity: a study with 3D chimeric tumor spheroids","volume":"13","author":"Goudar","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"bfacee21bib74","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201901462","article-title":"A dynamic 3D tumor spheroid chip enables more accurate nanomedicine uptake evaluation","volume":"6","author":"Zhuang","year":"2019","journal-title":"Adv. Sci."},{"key":"bfacee21bib75","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/acaa00","article-title":"The interface stiffness and topographic feature dictate interfacial invasiveness of cancer spheroids","volume":"15","author":"Mao","year":"2023","journal-title":"Biofabrication"},{"key":"bfacee21bib76","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/j.cell.2005.02.018","article-title":"Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues","volume":"121","author":"Hochedlinger","year":"2005","journal-title":"Cell"},{"key":"bfacee21bib77","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1016\/j.scib.2021.03.005","article-title":"Acidic pH transiently prevents the silencing of self-renewal and dampens microRNA function in embryonic stem cells","volume":"66","author":"Guo","year":"2021","journal-title":"Sci. Bull."},{"key":"bfacee21bib78","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/nprot.2012.153","article-title":"Production of hepatocyte-like cells from human pluripotent stem cells","volume":"8","author":"Hannan","year":"2013","journal-title":"Nat. Protocols"},{"key":"bfacee21bib79","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1002\/hep.23354","article-title":"Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells","volume":"51","author":"Si-Tayeb","year":"2010","journal-title":"Hepatology"},{"key":"bfacee21bib80","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1038\/s41565-020-0669-6","article-title":"Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR-Cas gene editing","volume":"15","author":"Cheng","year":"2020","journal-title":"Nat. Nanotechnol."},{"key":"bfacee21bib81","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1038\/nbt1310","article-title":"A ROCK inhibitor permits survival of dissociated human embryonic stem cells","volume":"25","author":"Watanabe","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"bfacee21bib82","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.stem.2010.06.018","article-title":"Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells","volume":"7","author":"Ohgushi","year":"2010","journal-title":"Cell Stem Cell"},{"key":"bfacee21bib83","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.jconrel.2015.04.032","article-title":"Recent progress in biomedical applications of Pluronic (PF127): pharmaceutical perspectives","volume":"209","author":"Akash","year":"2015","journal-title":"J. Control Release"},{"key":"bfacee21bib84","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1016\/j.cell.2020.04.010","article-title":"Adhesion protein structure, molecular affinities, and principles of cell-cell recognition","volume":"181","author":"Honig","year":"2020","journal-title":"Cell"},{"key":"bfacee21bib85","doi-asserted-by":"publisher","first-page":"18149","DOI":"10.3390\/ijms160818149","article-title":"A review of cell adhesion studies for biomedical and biological applications","volume":"16","author":"Khalili","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"bfacee21bib86","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1016\/j.cmet.2019.05.007","article-title":"Modeling steatohepatitis in humans with pluripotent stem cell-derived organoids","volume":"30","author":"Ouchi","year":"2019","journal-title":"Cell Metab."},{"key":"bfacee21bib87","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1016\/j.jhep.2019.06.030","article-title":"Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids","volume":"71","author":"Mun","year":"2019","journal-title":"J. Hepatol."},{"key":"bfacee21bib88","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.574047","article-title":"Liver organoids: recent developments, limitations and potential","volume":"8","author":"Harrison","year":"2021","journal-title":"Front. Med."},{"key":"bfacee21bib89","doi-asserted-by":"publisher","first-page":"1825","DOI":"10.1002\/hep.31857","article-title":"Functional characterization of organoids derived from irreversibly damaged liver of patients with NASH","volume":"74","author":"McCarron","year":"2021","journal-title":"Hepatology"},{"key":"bfacee21bib90","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1038\/s41556-019-0359-5","article-title":"Modelling liver cancer initiation with organoids derived from directly reprogrammed human hepatocytes","volume":"21","author":"Sun","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"bfacee21bib91","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312808","article-title":"Cytochrome P450 enzymes and drug metabolism in humans","volume":"22","author":"Zhao","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"bfacee21bib92","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1016\/j.jhep.2018.12.028","article-title":"Generation of hepatobiliary organoids from human induced pluripotent stem cells","volume":"70","author":"Wu","year":"2019","journal-title":"J. Hepatol."},{"key":"bfacee21bib93","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1038\/nprot.2016.170","article-title":"Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells","volume":"12","author":"Morizane","year":"2017","journal-title":"Nat. Protocols"},{"key":"bfacee21bib94","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1126\/science.aaw7894","article-title":"Organoids-on-a-chip","volume":"364","author":"Park","year":"2019","journal-title":"Science"},{"key":"bfacee21bib95","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/j.cmi.2016.03.026","article-title":"The refusal of the society to accept antibiotic toxicity: missing opportunities for therapy of severe infections","volume":"22","author":"Rolain","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"bfacee21bib96","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1080\/10408444.2021.2024496","article-title":"Toxicity induced by ciprofloxacin and enrofloxacin: oxidative stress and metabolism","volume":"51","author":"Badawy","year":"2021","journal-title":"Crit. Rev. Toxicol."},{"key":"bfacee21bib97","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.addr.2020.06.002","article-title":"Lipid nanoparticles for nucleic acid delivery: current perspectives","volume":"154\u2013155","author":"Samaridou","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"bfacee21bib98","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/s41596-022-00755-x","article-title":"Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery","volume":"18","author":"Wang","year":"2023","journal-title":"Nat. Protocols"},{"key":"bfacee21bib99","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.copbio.2021.09.016","article-title":"Principles for designing an optimal mRNA lipid nanoparticle vaccine","volume":"73","author":"Kon","year":"2022","journal-title":"Curr. Opin. Biotechnol."},{"key":"bfacee21bib100","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1056\/NEJMoa2035389","article-title":"Efficacy and safety of the mRNA-1273 SARS-CoV-2 vaccine","volume":"384","author":"Baden","year":"2021","journal-title":"N. Engl. J. Med."},{"key":"bfacee21bib101","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1038\/nrg3456","article-title":"Functional and evolutionary implications of gene orthology","volume":"14","author":"Gabaldon","year":"2013","journal-title":"Nat. Rev. Genet."},{"key":"bfacee21bib102","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1093\/bib\/bbr031","article-title":"When orthologs diverge between human and mouse","volume":"12","author":"Gharib","year":"2011","journal-title":"Brief. Bioinformatics"},{"key":"bfacee21bib103","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/s41580-020-00315-9","article-title":"Gene regulation by long non-coding RNAs and its biological functions","volume":"22","author":"Statello","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"bfacee21bib104","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.cell.2018.01.011","article-title":"Functional classification and experimental dissection of long noncoding RNAs","volume":"172","author":"Kopp","year":"2018","journal-title":"Cell"},{"key":"bfacee21bib105","doi-asserted-by":"publisher","first-page":"4618","DOI":"10.1242\/dev.110544","article-title":"The lncRNA Neat1 is required for corpus luteum formation and the establishment of pregnancy in a subpopulation of mice","volume":"141","author":"Nakagawa","year":"2014","journal-title":"Development"},{"key":"bfacee21bib106","doi-asserted-by":"publisher","first-page":"1844","DOI":"10.1261\/rna.047332.114","article-title":"The long noncoding RNA Neat1 is required for mammary gland development and lactation","volume":"20","author":"Standaert","year":"2014","journal-title":"RNA"},{"key":"bfacee21bib107","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1038\/s41416-021-01588-3","article-title":"Expression and functions of long non-coding RNA NEAT1 and isoforms in breast cancer","volume":"126","author":"Knutsen","year":"2022","journal-title":"Br. J. Cancer"},{"key":"bfacee21bib108","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1007\/s00109-020-01978-9","article-title":"Wnt signaling in kidney: the initiator or terminator?","volume":"98","author":"Meng","year":"2020","journal-title":"J. Mol. Med."},{"key":"bfacee21bib109","doi-asserted-by":"publisher","first-page":"2094","DOI":"10.1242\/dev.114215","article-title":"Orchestrating liver development","volume":"142","author":"Gordillo","year":"2015","journal-title":"Development"},{"key":"bfacee21bib110","doi-asserted-by":"publisher","first-page":"1490","DOI":"10.1126\/science.1161431","article-title":"Generation and regeneration of cells of the liver and pancreas","volume":"322","author":"Zaret","year":"2008","journal-title":"Science"},{"key":"bfacee21bib111","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1038\/s41568-018-0007-6","article-title":"Organoids in cancer research","volume":"18","author":"Drost","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"bfacee21bib112","doi-asserted-by":"publisher","first-page":"C151","DOI":"10.1152\/ajpcell.00120.2020","article-title":"A brief history of organoids","volume":"319","author":"Corro","year":"2020","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"bfacee21bib113","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.conctc.2018.08.001","article-title":"Factors associated with clinical trials that fail and opportunities for improving the likelihood of success: a review","volume":"11","author":"Fogel","year":"2018","journal-title":"Contemp. Clin. Trials Commun."},{"key":"bfacee21bib114","doi-asserted-by":"publisher","first-page":"eaax2537","DOI":"10.1126\/science.aax2537","article-title":"Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment","volume":"371","author":"Trujillo","year":"2021","journal-title":"Science"},{"key":"bfacee21bib115","doi-asserted-by":"publisher","first-page":"eabl6422","DOI":"10.1126\/science.abl6422","article-title":"Human TKTL1 implies greater neurogenesis in frontal neocortex of modern humans than Neanderthals","volume":"377","author":"Pinson","year":"2022","journal-title":"Science"},{"key":"bfacee21bib116","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1016\/j.bbagen.2013.10.035","article-title":"Evolutionary conservation of long non-coding RNAs; sequence, structure, function","volume":"1840","author":"Johnsson","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"bfacee21bib117","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.molcel.2009.06.027","article-title":"Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA","volume":"35","author":"Chen","year":"2009","journal-title":"Mol. Cell"},{"key":"bfacee21bib118","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1038\/s41587-020-0561-9","article-title":"Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors","volume":"38","author":"Anzalone","year":"2020","journal-title":"Nat. Biotechnol."}],"container-title":["Biofabrication"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T08:34:58Z","timestamp":1692606898000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/acee21"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,21]]},"references-count":118,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,8,21]]},"published-print":{"date-parts":[[2023,10,1]]}},"URL":"https:\/\/doi.org\/10.1088\/1758-5090\/acee21","relation":{},"ISSN":["1758-5082","1758-5090"],"issn-type":[{"value":"1758-5082","type":"print"},{"value":"1758-5090","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,21]]},"assertion":[{"value":"Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly","name":"article_title","label":"Article Title"},{"value":"Biofabrication","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2023 The Author(s). Published by IOP Publishing Ltd","name":"copyright_information","label":"Copyright Information"},{"value":"2023-05-01","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2023-08-08","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2023-08-21","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}