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Tissue specific hydrogels retain the individual biochemical composition of the extracellular matrix and thus promote the inherent cell\u00b4s physiological function. Hence, we created a decellularized lacrimal gland (LG) hydrogel (dLG-HG) meeting the requirements for a bioink as the basis of a LG model with potential for <jats:italic>in vitro<\/jats:italic> ADDE studies. Varying hydrolysis durations were compared to obtain dLG-HG with best possible physical and ultrastructural properties while preserving the original biochemical composition. A particular focus was placed on dLG-HG\u00b4s impact on viability and functionality of LG associated cell types with relevance for a future <jats:italic>in vitro<\/jats:italic> model in comparison to the unspecific single component hydrogel collagen type-I (Col) and the common cell culture substrate Matrigel. Proliferation of LG epithelial cells (EpC), LG mesenchymal stem cells, and endothelial cells cultured on dLG-HG was enhanced compared to culture on Matrigel. Most importantly with respect to a functional <jats:italic>in vitro<\/jats:italic> model, the secretion capacity of EpC cultured on dLG-HG was higher than that of EpC cultured on Col or Matrigel. In addition to these promising cell related properties, a rapid matrix metalloproteinase-dependent biodegradation was observed, which on the one hand suggests a lively cell\u2013matrix interaction, but on the other hand limits the cultivation period. Concluding, dLG-HG possesses decisive properties for the tissue engineering of a LG <jats:italic>in vitro<\/jats:italic> model such as cytocompatibility and promotion of secretion, making it superior to unspecific cell culture substrates. However, deceleration of biodegradation should be addressed in future experiments.<\/jats:p>","DOI":"10.1088\/1758-5090\/ad2082","type":"journal-article","created":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T22:23:01Z","timestamp":1705702981000},"page":"025008","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Enhancement of lacrimal gland cell function by decellularized lacrimal gland derived hydrogel"],"prefix":"10.1088","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1567-9282","authenticated-orcid":true,"given":"Katharina E","family":"Wiebe-Ben Zakour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sema","family":"Kaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia C","family":"Matros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael C","family":"Hacker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amina","family":"Cheikh-Rouhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Spaniol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Geerling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Witt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"bfad2082bib1","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1097\/ICU.0b013e32832b7578","article-title":"Dry eye in 2008","volume":"20","author":"Abelson","year":"2009","journal-title":"Curr. 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