{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T14:00:56Z","timestamp":1793109656612,"version":"4.5.0"},"reference-count":82,"publisher":"IOP Publishing","issue":"2","license":[{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,7]],"date-time":"2025-02-07T00:00:00Z","timestamp":1738886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"crossref","award":["788753"],"award-info":[{"award-number":["788753"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"crossref","award":["892389"],"award-info":[{"award-number":["892389"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ON Foundation","award":["21-053"],"award-info":[{"award-number":["21-053"]}]},{"name":"US-Ireland Research and Development Partnership","award":["17\/US\/3437"],"award-info":[{"award-number":["17\/US\/3437"]}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"crossref","award":["12\/RC\/2278"],"award-info":[{"award-number":["12\/RC\/2278"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Health Research Boards","award":["ILP-POR-2017-032"],"award-info":[{"award-number":["ILP-POR-2017-032"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biofabrication"],"published-print":{"date-parts":[[2025,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Osteomyelitis, a severe bone infection, is an extremely challenging complication in the repair of traumatic bone defects. Furthermore, the use of long-term high-dose antibiotics in standard treatment increases the risks of antibiotic resistance. Herein, an antibiotic-free, collagen silver-doped hydroxyapatite (coll-AgHA) scaffold reinforced with a 3D printed polycaprolactone (PCL) framework was developed with enhanced mechanical properties to be used in the repair of load-bearing defects with antimicrobial properties as a preventative measure against osteomyelitis. The AgHA particles were fabricated in varying Ag doses and loaded within freeze-dried collagen scaffolds at two concentrations. The optimised Ag dose (1.5 mol% Ag) and AgHA concentration (200 wt%) within the collagen scaffold demonstrated <jats:italic>in vitro<\/jats:italic> osteogenic and antibacterial properties against <jats:italic>S. aureus (S. aureus),<\/jats:italic> the main causative pathogen of osteomyelitis. The addition of the PCL framework to the coll-AgHA scaffolds significantly enhanced the compressive modulus from 4 to 12 MPa while maintaining high porosity as well as both pro-osteogenic and antibacterial properties. The reinforced coll-AgHA scaffolds were implanted <jats:italic>in vivo<\/jats:italic> and demonstrated enhanced bone repair, significantly greater vessel formation, and calcified tissue in a load-bearing critical sized defect in rats. Taken together, these results confirm the capacity of this novel biomaterial scaffold as a preventative measure against infection in bone repair for use in load-bearing defects, without the use of antibiotics.<\/jats:p>","DOI":"10.1088\/1758-5090\/adaf59","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T22:54:58Z","timestamp":1738104898000},"page":"025010","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Collagen silver-doped hydroxyapatite scaffolds reinforced with 3D printed frameworks for infection prevention and enhanced repair of load-bearing bone defects"],"prefix":"10.1088","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8551-6222","authenticated-orcid":true,"given":"Katelyn J","family":"Genoud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna M","family":"Sadowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7250-0299","authenticated-orcid":true,"given":"Rachael N","family":"Power","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara S","family":"Costard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily J","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2316-3000","authenticated-orcid":true,"given":"Austyn R","family":"Matherson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlyng G","family":"Gonzalez-Vazquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Lemoine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9346-7731","authenticated-orcid":true,"given":"Kian","family":"Eichholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierluca","family":"Pitacco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenton","family":"Cavanagh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orquidea","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciara M","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0060-4213","authenticated-orcid":true,"given":"Caroline M","family":"Curtin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4091-0992","authenticated-orcid":true,"given":"Daniel J","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2030-8005","authenticated-orcid":true,"given":"Fergal J","family":"O\u2019Brien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2025,2,7]]},"reference":[{"key":"bfadaf59bib1","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.mattod.2020.12.018","article-title":"Bone biomaterials for overcoming antimicrobial resistance: advances in non-antibiotic antimicrobial approaches for regeneration of infected osseous tissue","volume":"46","author":"Sadowska","year":"2021","journal-title":"Mater. Today"},{"key":"bfadaf59bib2","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.3390\/diagnostics12051289","article-title":"Fracture related infections and their risk factors for treatment failure\u2014a major trauma centre perspective","volume":"12","author":"Lu","year":"2022","journal-title":"Diagnostics"},{"key":"bfadaf59bib3","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/S0140-6736(22)00087-3","article-title":"The overlooked pandemic of antimicrobial resistance","volume":"399","author":"Laxminarayan","year":"2022","journal-title":"Lancet"},{"key":"bfadaf59bib4","article-title":"Global antimicrobial resistance and use surveillance system","author":"Antimicrobial Resistance Division (AMR)","year":"2022"},{"key":"bfadaf59bib5","doi-asserted-by":"crossref","DOI":"10.1097\/00003086-200202000-00009","article-title":"Properties of osteoconductive biomaterials: calcium phosphates","author":"LeGeros","year":"2002"},{"key":"bfadaf59bib6","first-page":"p 600","article-title":"Chapter 9: calcium phosphate ceramics for bone tissue engineering","author":"Wolke","year":"2007"},{"key":"bfadaf59bib7","doi-asserted-by":"publisher","first-page":"6083","DOI":"10.1016\/j.apsusc.2010.03.124","article-title":"Synthesis, characterization and antimicrobial activity of copper and zinc-doped hydroxyapatite nanopowders","volume":"256","author":"Stani\u0107","year":"2010","journal-title":"Appl. Surf. Sci."},{"key":"bfadaf59bib8","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/j.carbpol.2017.09.095","article-title":"Fabrication and characterization of copper(II)-chitosan complexes as antibiotic-free antibacterial biomaterial","volume":"179","author":"Gritsch","year":"2018","journal-title":"Carbohydrate Polym."},{"key":"bfadaf59bib9","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1155\/MBD.1994.467","article-title":"Antibacterial Silver","volume":"1","author":"Clement","year":"1994","journal-title":"Met. Based Drugs"},{"key":"bfadaf59bib10","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1089\/sur.2008.9941","article-title":"History of the medical use of silver","volume":"10","author":"Alexander","year":"2009","journal-title":"Surg. Infect."},{"key":"bfadaf59bib11","doi-asserted-by":"publisher","first-page":"4507","DOI":"10.1016\/j.ceramint.2013.08.125","article-title":"Characterization, antibacterial and in vitro compatibility of zinc\u2013silver doped hydroxyapatite nanoparticles prepared through microwave synthesis","volume":"40","author":"Iqbal","year":"2014","journal-title":"Ceram. Int."},{"key":"bfadaf59bib12","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.msec.2017.11.003","article-title":"Mesoporous silica-based bioactive glasses for antibiotic-free antibacterial applications","volume":"83","author":"Kaya","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"bfadaf59bib13","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.msec.2015.05.078","article-title":"Ultra-trace silver-doped hydroxyapatite with non-cytotoxicity and effective antibacterial activity","volume":"55","author":"Shi","year":"2015","journal-title":"Mater. Sci. Eng. C"},{"key":"bfadaf59bib14","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/j.msec.2015.08.061","article-title":"Strontium incorporation to optimize the antibacterial and biological characteristics of silver-substituted hydroxyapatite coating","volume":"58","author":"Geng","year":"2016","journal-title":"Mater. Sci. Eng. C"},{"key":"bfadaf59bib15","doi-asserted-by":"publisher","first-page":"1781","DOI":"10.1039\/C9TB02710F","article-title":"Substituted hydroxyapatite coatings of bone implants","volume":"8","author":"Arcos","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"bfadaf59bib16","doi-asserted-by":"publisher","first-page":"27","DOI":"10.4028\/www.scientific.net\/KEM.493-494.27","article-title":"Silver-doped apatite as a bioactive and an antimicrobial bone material","volume":"493","author":"Thian","year":"2012","journal-title":"Key Eng. Mater."},{"key":"bfadaf59bib17","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1002\/jor.22903","article-title":"Silver oxide\u2010containing hydroxyapatite coating supports osteoblast function and enhances implant anchorage strength in rat femur","volume":"33","author":"Shuichi","year":"2015","journal-title":"J. Orthop. Res."},{"key":"bfadaf59bib18","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1166\/jbn.2017.2387","article-title":"Preparation and antibacterial properties of silver-doped nanoscale hydroxyapatite pastes for bone repair and augmentation","volume":"13","author":"Wilcock","year":"2017","journal-title":"J. Biomed. Nanotechnol."},{"key":"bfadaf59bib19","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1021\/acsbiomaterials.5b00170","article-title":"Is nano-silver safe within bioactive hydroxyapatite composites?","volume":"1","author":"Vukomanovi\u0107","year":"2015","journal-title":"ACS Biomater. Sci. Eng."},{"key":"bfadaf59bib20","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1186\/1556-276X-7-324","article-title":"Antibacterial activity of silver-doped hydroxyapatite nanoparticles against gram-positive and gram-negative bacteria","volume":"7","author":"Ciobanu","year":"2012","journal-title":"Nanoscale Res. Lett."},{"key":"bfadaf59bib21","doi-asserted-by":"publisher","first-page":"23477","DOI":"10.1021\/acsami.6b06596","article-title":"Content-dependent osteogenic response of nanohydroxyapatite: an in vitro and in vivo assessment within collagen-based scaffolds","volume":"8","author":"Cunniffe","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"bfadaf59bib22","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1002\/adhm.201400397","article-title":"Combinatorial gene therapy accelerates bone regeneration: non-viral dual delivery of VEGF and BMP2 in a collagen-nanohydroxyapatite scaffold","volume":"4","author":"Curtin","year":"2015","journal-title":"Adv. Healthcare Mater."},{"key":"bfadaf59bib23","doi-asserted-by":"publisher","first-page":"218","DOI":"10.22203\/eCM.v020a18","article-title":"Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration","volume":"20","author":"Gleeson","year":"2010","journal-title":"Eur. Cell Mater."},{"key":"bfadaf59bib24","doi-asserted-by":"publisher","first-page":"3","DOI":"10.3233\/THC-2007-15102","article-title":"The effect of pore size on permeability and cell attachment in collagen scaffolds for tissue engineering","volume":"15","author":"O\u2019Brien","year":"2007","journal-title":"Technol. Health Care"},{"key":"bfadaf59bib25","doi-asserted-by":"publisher","first-page":"377","DOI":"10.4161\/cam.4.3.11747","article-title":"Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds","volume":"4","author":"Murphy","year":"2010","journal-title":"Cell Adh. Migr."},{"key":"bfadaf59bib26","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202307639","article-title":"A multifunctional scaffold for bone infection treatment by delivery of microRNA therapeutics combined with antimicrobial nanoparticles","volume":"36","author":"Sadowska","year":"2024","journal-title":"Adv. Mater."},{"key":"bfadaf59bib27","doi-asserted-by":"publisher","first-page":"e00084","DOI":"10.1128\/CMR.00084-17","article-title":"Staphylococcal osteomyelitis: disease progression, treatment challenges, and future directions","volume":"31","author":"Kavanagh","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"bfadaf59bib28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jsamd.2020.01.007","article-title":"Porous scaffolds for bone regeneration","volume":"5","author":"Abbasi","year":"2020","journal-title":"J. Sci."},{"key":"bfadaf59bib29","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1038\/nmat1421","article-title":"Porous scaffold design for tissue engineering","volume":"4","author":"Hollister","year":"2005","journal-title":"Nat. Mater."},{"key":"bfadaf59bib30","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202403641","article-title":"Recent advances in 3D printing of smart scaffolds for bone tissue engineering and regeneration","volume":"36","author":"Yuan","year":"2024","journal-title":"Adv. Mater."},{"key":"bfadaf59bib31","doi-asserted-by":"publisher","first-page":"158","DOI":"10.22203\/eCM.v045a11","article-title":"Development of a 3D-printed bioabsorbable composite scaffold with mechanical properties suitable for treating large, load-bearingarticular cartilage defects","volume":"45","author":"Joyce","year":"2023","journal-title":"Eur. Cell. Mater."},{"key":"bfadaf59bib32","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1187500","article-title":"Development of tissue-engineered tracheal scaffold with refined mechanical properties and vascularisation for tracheal regeneration","volume":"11","author":"Khalid","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"bfadaf59bib33","doi-asserted-by":"publisher","DOI":"10.3791\/55343","article-title":"Rapid mix preparation of bioinspired nanoscale hydroxyapatite for biomedical applications","volume":"120","author":"Wilcock","year":"2017","journal-title":"JoVE"},{"key":"bfadaf59bib34","doi-asserted-by":"publisher","first-page":"2297","DOI":"10.3390\/ma8052297","article-title":"Process optimisation to control the physico-chemical characteristics of biomimetic nanoscale hydroxyapatites prepared using wet chemical precipitation","volume":"8","author":"Gentile","year":"2015","journal-title":"Materials"},{"key":"bfadaf59bib35","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1021\/j150449a003","article-title":"Refractive index of ethanol\u2013water mixtures and density and refractive index of ethanol\u2013water\u2013ethyl ether mixtures","volume":"50","author":"Scott","year":"1946","journal-title":"J. Phys. Chem."},{"key":"bfadaf59bib36","doi-asserted-by":"publisher","first-page":"6981","DOI":"10.1039\/C2RA20684F","article-title":"The toxic effect of silver ions and silver nanoparticles towards bacteria and human cells occurs in the same concentration range","volume":"2","author":"Greulich","year":"2012","journal-title":"RSC Adv."},{"key":"bfadaf59bib37","doi-asserted-by":"publisher","first-page":"58","DOI":"10.3390\/jfb11030058","article-title":"Electrospun scaffolds containing silver-doped hydroxyapatite with antimicrobial properties for applications in orthopedic and dental bone surgery","volume":"11","author":"Paterson","year":"2020","journal-title":"J. Funct. Biomater."},{"key":"bfadaf59bib38","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1016\/S0142-9612(03)00630-6","article-title":"Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds","volume":"25","author":"O\u2019Brien","year":"2004","journal-title":"Biomaterials"},{"key":"bfadaf59bib39","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1089\/ten.TEA.2010.0590","article-title":"Crosslinking and mechanical properties significantly influence cell attachment, proliferation, and migration within collagen glycosaminoglycan scaffolds","volume":"17","author":"Haugh","year":"2011","journal-title":"Tissue Eng. A"},{"key":"bfadaf59bib40","author":"Lemoine","year":"2020"},{"key":"bfadaf59bib41","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1002\/jbm.a.32366","article-title":"Surface modification of PCL-TCP scaffolds improve interfacial mechanical interlock and enhance early bone formation: an in vitro and in vivo characterization","volume":"92","author":"Yeo","year":"2010","journal-title":"J. Biomed. Mater. Res. A"},{"key":"bfadaf59bib42","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.biomaterials.2016.02.026","article-title":"Appropriate density of PCL nano-fiber sheath promoted muscular remodeling of PGS\/PCL grafts in arterial circulation","volume":"88","author":"Yang","year":"2016","journal-title":"Biomaterials"},{"key":"bfadaf59bib43","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/j.jconrel.2011.11.026","article-title":"The development of non-viral gene-activated matrices for bone regeneration using polyethyleneimine (PEI) and collagen-based scaffolds","volume":"158","author":"Tierney","year":"2012","journal-title":"J. Control Release"},{"key":"bfadaf59bib44","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1111\/j.1432-0436.1990.tb00619.x","article-title":"Regulation of alkaline phosphatase and alpha 2(I) procollagen synthesis during early intramembranous bone formation in the rat mandible","volume":"44","author":"Zernik","year":"1990","journal-title":"Differentiation"},{"key":"bfadaf59bib45","article-title":"M100Ed32","author":"Lewis","year":"2022","edition":"32nd edn"},{"key":"bfadaf59bib46","doi-asserted-by":"publisher","first-page":"666","DOI":"10.3389\/fendo.2018.00666","article-title":"An optimized approach to perform bone histomorphometry","volume":"9","author":"Malhan","year":"2018","journal-title":"Front. Endocrinol."},{"key":"bfadaf59bib47","doi-asserted-by":"publisher","DOI":"10.4161\/biom.27993","article-title":"Comprehensive histological evaluation of bone implants","volume":"4","author":"Rentsch","year":"2014","journal-title":"Biomatter"},{"key":"bfadaf59bib48","doi-asserted-by":"publisher","first-page":"11002","DOI":"10.1021\/la0621665","article-title":"Apparent solubility of hydroxyapatite in aqueous medium and its influence on the morphology of nanocrystallites with precipitation temperature","volume":"22","author":"Prakash","year":"2006","journal-title":"Langmuir"},{"key":"bfadaf59bib49","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1097\/BCO.0b013e3282630851","article-title":"The role of alkaline phosphatase in mineralization","volume":"18","author":"Golub","year":"2007","journal-title":"Curr. Opin. Orthop."},{"key":"bfadaf59bib50","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1533\/9781845696610.2.271","article-title":"Cements as bone repair materials","author":"Ginebra","year":"2009"},{"key":"bfadaf59bib51","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202300932","article-title":"Silver-containing biomaterials for biomedical hard tissue implants","volume":"12","author":"Shao","year":"n.d.","journal-title":"Adv. Healthcare Mater."},{"key":"bfadaf59bib52","doi-asserted-by":"publisher","first-page":"7562","DOI":"10.1021\/acsabm.0c00801","article-title":"Hydroxyapatite particle shape and size influence MSC osteogenesis by directing the macrophage phenotype in collagen-hydroxyapatite scaffolds","volume":"3","author":"Sridharan","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"bfadaf59bib53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-017-03086-0","article-title":"The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation","volume":"7","author":"Lebre","year":"2017","journal-title":"Sci. Rep."},{"key":"bfadaf59bib54","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/978-1-4614-1080-5_2","article-title":"Matrix stiffness: a regulator of cellular behavior and tissue formation","author":"Mason","year":"2012"},{"key":"bfadaf59bib55","doi-asserted-by":"publisher","first-page":"1139","DOI":"10.1126\/science.1116995","article-title":"Tissue cells feel and respond to the stiffness of their substrate","volume":"310","author":"Discher","year":"2005","journal-title":"Science"},{"key":"bfadaf59bib56","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.cell.2006.06.044","article-title":"Matrix elasticity directs stem cell lineage specification","volume":"126","author":"Engler","year":"2006","journal-title":"Cell"},{"key":"bfadaf59bib57","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1111\/joa.12262","article-title":"Effect of different hydroxyapatite incorporation methods on the structural and biological properties of porous collagen scaffolds for bone repair","volume":"227","author":"Ryan","year":"2015","journal-title":"J. Anat."},{"key":"bfadaf59bib58","author":"Ryan","year":"2019"},{"key":"bfadaf59bib59","doi-asserted-by":"publisher","first-page":"5474","DOI":"10.1016\/j.biomaterials.2005.02.002","article-title":"Porosity of 3D biomaterial scaffolds and osteogenesis","volume":"26","author":"Karageorgiou","year":"2005","journal-title":"Biomaterials"},{"key":"bfadaf59bib60","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.yrtph.2018.08.007","article-title":"Toxicity of silver ions, metallic silver, and silver nanoparticle materials after in vivo dermal and mucosal surface exposure: a review","volume":"98","author":"Hadrup","year":"2018","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"bfadaf59bib61","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2019.121974","article-title":"Silver nanoparticles: toxicity in model organisms as an overview of its hazard for human health and the environment","volume":"390","author":"Tortella","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"bfadaf59bib62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.114636","article-title":"Synthesis, applications, toxicity and toxicity mechanisms of silver nanoparticles: a review","volume":"253","author":"Nie","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"bfadaf59bib63","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/ab28e4","article-title":"Silver and gold doped hydroxyapatite nanocomposites for enhanced bone regeneration","volume":"14","author":"Kumar","year":"2019","journal-title":"Biomed. Mater."},{"key":"bfadaf59bib64","doi-asserted-by":"publisher","first-page":"4510","DOI":"10.1016\/j.apsusc.2010.12.113","article-title":"Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering","volume":"257","author":"Stani\u0107","year":"2011","journal-title":"Appl. Surf. Sci."},{"key":"bfadaf59bib65","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.msec.2018.04.076","article-title":"Synthesis of monophasic Ag doped hydroxyapatite and evaluation of antibacterial activity","volume":"90","author":"Riaz","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"bfadaf59bib66","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.actbio.2016.02.004","article-title":"Increasing the strength and bioactivity of collagen scaffolds using customizable arrays of 3D-printed polymer fibers","volume":"33","author":"Mozdzen","year":"2016","journal-title":"Acta Biomater."},{"key":"bfadaf59bib67","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.jmbbm.2016.10.017","article-title":"Modifying the strength and strain concentration profile within collagen scaffolds using customizable arrays of poly-lactic acid fibers","volume":"66","author":"Mozdzen","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"bfadaf59bib68","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.actbio.2020.11.028","article-title":"Inclusion of a 3D-printed Hyperelastic Bone mesh improves mechanical and osteogenic performance of a mineralized collagen scaffold","volume":"121","author":"Dewey","year":"2021","journal-title":"Acta Biomater."},{"key":"bfadaf59bib69","doi-asserted-by":"publisher","first-page":"2950","DOI":"10.3390\/polym13172950","article-title":"Effect of polymeric matrix stiffness on osteogenic differentiation of mesenchymal stem\/progenitor cells","volume":"13","author":"El-Rashidy","year":"2021","journal-title":"Concise Rev. Polym."},{"key":"bfadaf59bib70","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1186\/s13287-018-0798-0","article-title":"Extracellular matrix stiffness controls osteogenic differentiation of mesenchymal stem cells mediated by integrin \u03b15","volume":"9","author":"Sun","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"bfadaf59bib71","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.bioactmat.2021.12.005","article-title":"An overview of substrate stiffness guided cellular response and its applications in tissue regeneration","volume":"15","author":"Yi","year":"2022","journal-title":"Bioact. Mater."},{"key":"bfadaf59bib72","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.1002\/jor.22357","article-title":"Silver oxide-containing hydroxyapatite coating has in vivo antibacterial activity in the rat tibia","volume":"31","author":"Akiyama","year":"2013","journal-title":"J. Orthop. Res."},{"key":"bfadaf59bib73","doi-asserted-by":"publisher","first-page":"1498","DOI":"10.1016\/j.arth.2015.12.034","article-title":"First clinical experience with thermal-sprayed silver oxide\u2013containing hydroxyapatite coating implant","volume":"31","author":"Eto","year":"2016","journal-title":"J. Arthroplast."},{"key":"bfadaf59bib74","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.actbio.2021.03.003","article-title":"3D bioprinting of prevascularised implants for the repair of critically-sized bone defects","volume":"126","author":"Nulty","year":"2021","journal-title":"Acta Biomater."},{"key":"bfadaf59bib75","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac88a1","article-title":"Scaffold microarchitecture regulates angiogenesis and the regeneration of large bone defects","volume":"14","author":"Eichholz","year":"2022","journal-title":"Biofabrication"},{"key":"bfadaf59bib76","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.actbio.2022.07.037","article-title":"3D bioprinting of cartilaginous templates for large bone defect healing","volume":"156","author":"Pitacco","year":"2022","journal-title":"Acta Biomater."},{"key":"bfadaf59bib77","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.bioactmat.2021.07.030","article-title":"Fabrication of a bio-instructive scaffold conferred with a favorable microenvironment allowing for superior implant osseointegration and accelerated in situ vascularized bone regeneration via type H vessel formation","volume":"9","author":"He","year":"2022","journal-title":"Bioact. Mater."},{"key":"bfadaf59bib78","doi-asserted-by":"publisher","first-page":"eabb5093","DOI":"10.1126\/sciadv.abb5093","article-title":"3D bioprinting spatiotemporally defined patterns of growth factors to tightly control tissue regeneration","volume":"6","author":"Freeman","year":"2020","journal-title":"Sci. Adv."},{"key":"bfadaf59bib79","doi-asserted-by":"publisher","first-page":"17699","DOI":"10.1039\/C9NR05730G","article-title":"Stimulation of osteogenesis and angiogenesis by micro\/nano hierarchical hydroxyapatite via macrophage immunomodulation","volume":"11","author":"Yang","year":"2019","journal-title":"Nanoscale"},{"key":"bfadaf59bib80","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1080\/17435390.2016.1214763","article-title":"Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio","volume":"10","author":"Gao","year":"2016","journal-title":"Nanotoxicology"},{"key":"bfadaf59bib81","doi-asserted-by":"publisher","first-page":"1637","DOI":"10.1016\/j.jvs.2013.02.012","article-title":"Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation","volume":"58","author":"Jeanmonod","year":"2013","journal-title":"J. Vasc. Surg."},{"key":"bfadaf59bib82","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.toxlet.2011.05.1033","article-title":"Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles","volume":"205","author":"Kang","year":"2011","journal-title":"Toxicol. Lett."}],"container-title":["Biofabrication"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:08:28Z","timestamp":1740132508000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/1758-5090\/adaf59"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,7]]},"references-count":82,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2,7]]},"published-print":{"date-parts":[[2025,4,1]]}},"URL":"https:\/\/doi.org\/10.1088\/1758-5090\/adaf59","relation":{},"ISSN":["1758-5082","1758-5090"],"issn-type":[{"value":"1758-5082","type":"print"},{"value":"1758-5090","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,7]]},"assertion":[{"value":"Collagen silver-doped hydroxyapatite scaffolds reinforced with 3D printed frameworks for infection prevention and enhanced repair of load-bearing bone defects","name":"article_title","label":"Article Title"},{"value":"Biofabrication","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2025 The Author(s). Published by IOP Publishing Ltd","name":"copyright_information","label":"Copyright Information"},{"value":"2024-07-24","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2025-01-28","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2025-02-07","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}