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While traditional DLP systems rely on light-emitting diodes (LEDs), their limited power spectral density, high etendue, and spectral inefficiency constrain their performance in resolution, dynamic range, printing time, and cell viability. This study proposes and evaluates a dual-laser DLP system to overcome these limitations and enhance bioprinting performance. The proposed dual-laser system resulted in a twofold increase in resolution and a twelvefold reduction in printing time compared to the LED system. The system\u2019s capability was evaluated by printing three distinct designs, achieving a maximum percentage error of 1.16% and a minimum of 0.02% in accurately reproducing complex structures. Further, the impact of exposure times (10\u201330 s) and light intensities (0.044\u20130.11 mW mm<jats:sup>\u22122<\/jats:sup>) on the viability and morphology of 3T3 fibroblasts in GelMA and GelMA-poly(ethylene glycol) diacrylate (PEGDA) hydrogels is assessed. The findings reveal a clear relationship between longer exposure times and reduced cell viability. On day 7, samples exposed for extended periods exhibited the lowest metabolic activity and cell density, with differences of \u223c40% between treatments. However, all samples show recovery by day 7, with GelMA samples exhibiting up to a sixfold increase in metabolic activity and GelMA-PEGDA samples showing up to a twofold increase. In contrast, light intensity variations had a lesser effect, with a maximum variation of 15% in cell viability. We introduced a segmented printing method to mitigate over-crosslinking and enhance the dynamic range, utilizing an adaptive segmentation control strategy. This method, demonstrated by printing a bronchial model with a 14.43x compression ratio, improved resolution and maintained cell viability up to 90% for GelMA and 85% for GelMA-PEGDA during 7 d of culture. The proposed dual-laser system and adaptive segmentation method were confirmed through successful prints with diverse bio-inks and complex structures, underscoring its advantages over traditional LED systems in advancing 3D bioprinting.<\/jats:p>","DOI":"10.1088\/1758-5090\/adbc22","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T22:52:36Z","timestamp":1741042356000},"page":"025026","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["High-resolution bioprinting of complex bio-structures via engineering of the photopatterning approaches and adaptive segmentation"],"prefix":"10.1088","volume":"17","author":[{"given":"Ceren","family":"Babayigit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7695-460X","authenticated-orcid":true,"given":"Jorge Alfonso","family":"Tavares-Negrete","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4528-3601","authenticated-orcid":true,"given":"Rahim","family":"Esfandyarpour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-1107","authenticated-orcid":true,"given":"Ozdal","family":"Boyraz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2025,3,13]]},"reference":[{"key":"bfadbc22bib1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac2d78","article-title":"Multi-material digital light processing bioprinting of hydrogel-based microfluidic chips","volume":"14","author":"Bhusal","year":"2021","journal-title":"Biofabrication"},{"key":"bfadbc22bib2","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202201926","article-title":"A novel 3D-bioprinting technology of orderly extruded multi-materials via photopolymerization","volume":"8","author":"Tavares-Negrete","year":"2023","journal-title":"Adv. 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