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Here, we present a microfluidic biofabrication strategy to engineer vascularized endometrial micro-organoids that recapitulate the cellular complexity and function of native tissue. By co-encapsulating human endometrial stromal cells, epithelial organoids, and endothelial cells (HUVECs) in biocompatible hydrogel microspheres, we created 3D constructs supporting hormone responsiveness, decidualization, and pathological remodeling upon transforming growth factor-\n                    <jats:italic>\u03b2<\/jats:italic>\n                    stimulation. Transcriptomic profiling and single-cell sequencing revealed that the presence of endothelial cells alleviated hypoxia-induced inflammation and promoted epithelial homeostasis.\n                    <jats:italic>In vivo<\/jats:italic>\n                    transplantation into a murine IUA model led to improved engraftment, endometrial regeneration, and fertility recovery. This vascularized organoid system offers a scalable and translational platform for endometrial repair and disease modeling, highlighting the promise of biofabrication in reproductive regenerative medicine.\n                  <\/jats:p>","DOI":"10.1088\/1758-5090\/ae4f26","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T22:52:17Z","timestamp":1773096737000},"page":"025011","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["A microfluidic-engineered vascularized endometrium micro-organoid platform for functional repair of intrauterine adhesion"],"prefix":"10.1088","volume":"18","author":[{"given":"Weijia","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5494-6948","authenticated-orcid":true,"given":"Yongdong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6555-8203","authenticated-orcid":true,"given":"Fanxuan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangying","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biya","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8615-7196","authenticated-orcid":true,"given":"Songying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"bfae4f26bib1","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.actbio.2021.04.032","type":"journal-article","article-title":"In vitro modelling of the physiological and diseased female reproductive system","volume":"132","author":"Stejskalova","year":"2021","journal-title":"Acta Biomater."},{"key":"bfae4f26bib2","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1089\/ten.tea.2020.0022","type":"journal-article","article-title":"Microphysiological modeling of the human endometrium","volume":"26","author":"Campo","year":"2020","journal-title":"Tissue Eng. 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