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Here, we demonstrate the feasibility of Sequential Additive Biofabrication Extended over Real-time (SABER), a bioprinting strategy that introduces time as an explicit design variable by enabling multi-day additive fabrication of soft-hydrogel constructs supported by continuous perfusion culture. SABER integrates a thermoresponsive methylcellulose-agarose support material, which allows high-fidelity collagen deposition at low temperatures and mechanical stabilization at culture temperatures, with a custom bioreactor that permits direct through-tissue perfusion. We demonstrate feasibility of multi-day fabrication of layered constructs, nested geometries, suspended internal features, a miniaturized acellular human heart model with preserved architectural fidelity, and a 10-layer approximately 1 cm thick construct demonstrating scalability beyond single-session practical limits. SABER supports perfusion culture of thick cell-laden constructs, enabling printed cardiac tissues to remain viable and contractile after one week of perfusion culture and permitting\n                    <jats:italic>in situ<\/jats:italic>\n                    differentiation of bioprinted iPS cells into cardiomyocytes. These findings establish SABER as a proof-of-concept platform for time-resolved, perfused bioprinting of soft hydrogel constructs, introducing time as an explicit design variable in biofabrication and providing a foundation for future work exploring multi-day fabrication workflows at scales and with temporal complexity not supported by existing single-session approaches.\n                  <\/jats:p>","DOI":"10.1088\/1758-5090\/ae8a86","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T22:51:33Z","timestamp":1784069493000},"page":"045008","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["SABER bioprinting: a temporal platform for multiday assembly of engineered tissues"],"prefix":"10.1088","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9229-4012","authenticated-orcid":true,"given":"Joshua R","family":"Gershlak","sequence":"first","affiliation":[{"name":"Massachusetts General Hospital"},{"name":"Harvard Medical School"},{"name":"Harvard University"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Shoaib A","family":"Goraya","sequence":"additional","affiliation":[{"name":"Harvard Medical School"},{"name":"Mass General Brigham"},{"name":"Brigham and Women\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7591-0362","authenticated-orcid":true,"given":"Marie","family":"Billaud","sequence":"additional","affiliation":[{"name":"Harvard Medical School"},{"name":"Mass General Brigham"},{"name":"Brigham and Women\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Farhad R","family":"Nezami","sequence":"additional","affiliation":[{"name":"Harvard Medical School"},{"name":"Mass General Brigham"},{"name":"Brigham and Women\u2019s Hospital"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Harald C","family":"Ott","sequence":"additional","affiliation":[{"name":"Massachusetts General Hospital"},{"name":"Harvard Medical School"},{"name":"United Therapeutics"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"266","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"bfae8a86bib1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trre.2020.100585","type":"journal-article","article-title":"Organ donation in the US and Europe: the supply vs demand imbalance","volume":"35","author":"Lewis","year":"2021","journal-title":"Transplant. 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