{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:58:58Z","timestamp":1780102738674,"version":"3.54.0"},"reference-count":29,"publisher":"IOP Publishing","issue":"3","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Methods Appl. Fluoresc."],"published-print":{"date-parts":[[2019,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    InP\/ZnS quantum dot (QD)-based fluorescent probe for directly sensitive and selective detection of horseradish peroxidase (HRP) was reported herein. Fluorescence of InP\/ZnS QDs was statically quenched by HRP, due to the ground state complex formation of InP\/ZnS QDs with HRP. Such ground state complex formation between InP\/ZnS QDs and HRP reduced both the\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -helix content and the melting temperature of HRP. Several key factors including InP\/ZnS QDs concentration, buffer pH value, ionic strength, reaction temperature, and reaction time those affected the analytical performance of InP\/ZnS QDs in HRP determination were investigated thoroughly. Under the optimal conditions, fluorescence intensity of InP\/ZnS QDs was linearly decreased with the increasing of HRP concentration during the range of 1.0\u00a0\u00d7\u00a010\n                    <jats:sup>\u22129<\/jats:sup>\n                    M\u00a0\u223c\u00a03.0\u00a0\u00d7\u00a010\n                    <jats:sup>\u22128<\/jats:sup>\n                    M (0.01 U ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00a0\u223c\u00a00.3 U ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) with the detection limit as low as 1.2\u00a0\u00d7\u00a010\n                    <jats:sup>\u221210<\/jats:sup>\n                    M (1.2 mU ml\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). The present method showed excellent selectivity for HRP over some amino acids, nucleotides, and common proteins. This method was utilized to detect HRP in synthetic samples successfully.\n                  <\/jats:p>","DOI":"10.1088\/2050-6120\/aaff92","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T17:16:27Z","timestamp":1547745387000},"page":"035008","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["InP\/ZnS quantum dot-based fluorescent probe for directly sensitive and selective detection of horseradish peroxidase"],"prefix":"10.1088","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1379-6223","authenticated-orcid":false,"given":"Erli","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiandong","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lumin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2019,6,26]]},"reference":[{"key":"mafaaff92bib1","doi-asserted-by":"publisher","first-page":"14737","DOI":"10.1002\/anie.201705650","type":"journal-article","article-title":"Versatile tri(pyrazolyl)phosphanes as phosphorus precursors for the synthesis of highly emitting InP\/ZnS quantum dots","volume":"56","author":"Panzer","year":"2017","journal-title":"Angew. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2018-08-30","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2019-01-17","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2019-06-26","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}