{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:42:46Z","timestamp":1787913766936,"version":"build-2784847793"},"reference-count":21,"publisher":"IOP Publishing","issue":"1","license":[{"start":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T00:00:00Z","timestamp":1698192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/page\/copyright"},{"start":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T00:00:00Z","timestamp":1698192000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["61861010"],"award-info":[{"award-number":["61861010"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Nature Science Foundation of Guangxi Province","award":["2018GXNSFAA281319"],"award-info":[{"award-number":["2018GXNSFAA281319"]}]},{"name":"Project of Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes","award":["CEMC2022002"],"award-info":[{"award-number":["CEMC2022002"]}]},{"name":"Project of Education Department of Guangdong Province","award":["2019KTSCX091"],"award-info":[{"award-number":["2019KTSCX091"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Methods Appl. Fluoresc."],"published-print":{"date-parts":[[2024,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Efficient and rapid detection of angiotensin-converting enzyme (ACE) activity is important for preventing hypertension and the discovery of new angiotensin-converting enzyme inhibitors (ACEI). In this work, a single-excitation and double-emission biomass-derived carbon quantum dots (CQDs) was prepared and applied for ratiometric fluorescence detection of ACE. Fresh banyan leaves were extracted with ethanol and acetone, and the extracted solution was used as the precursor to produce the carbon quantum dots (BL-CQDs) with single-excitation and double-emission properties. The synthesized BL-CQDs is about 1.7 nm, has a graphene-like structure, contains a variety of hydrophilic functional groups on the surface, and has good fluorescence properties. Its fluorescence intensity ratio (I<jats:sub>677<\/jats:sub>\/I<jats:sub>460<\/jats:sub>) is linear with ACE activity in the range of 0.02\u20130.8 U l<jats:sup>\u22121<\/jats:sup>. The regression equation is <jats:italic>\u25b3F<\/jats:italic>=2.5371 <jats:italic>C<\/jats:italic>\n                  <jats:sub>\n                     <jats:italic>ACE<\/jats:italic>\n                  <\/jats:sub>-0.0311. The method was successfully applied to the determination of ACE activity in pig lung and human serum, and the inhibitory efficiency of the flavonoid extract and captopril tablets on ACE activity was also investigated, which can be applied to the screening of ACEI. The survival rate and fluorescence imaging of Bel-7404 cells under the condition of high concentration BL-CQDs showed BL-CQDs had low cytotoxicity and good biocompatibility. These results indicate that the BL-CQDs can be used as an excellent fluorescent probe, providing a new method for screening ACE activity and plant-derived ACEI.<\/jats:p>","DOI":"10.1088\/2050-6120\/ad02dd","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T22:38:27Z","timestamp":1697150307000},"page":"015004","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Ratiometric fluorescence detection of the angiotensin-converting enzyme via single-excitation and double-emission biomass-derived carbon quantum dots"],"prefix":"10.1088","volume":"12","author":[{"given":"Zhihua","family":"Zhan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3543-1690","authenticated-orcid":false,"given":"Mingyue","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guocheng","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2023,10,25]]},"reference":[{"key":"mafad02ddbib1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpc.2021.100230","article-title":"Age and sex disparities in hypertension control: the multi-ethnic study of atherosclerosis (MESA)","volume":"8","author":"Osude","year":"2021","journal-title":"Am. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2023-06-12","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2023-10-12","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2023-10-25","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}