{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:20:24Z","timestamp":1788355224457,"version":"build-2803163510"},"reference-count":41,"publisher":"IOP Publishing","issue":"4","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/questions\/alternative-author-rights-policies\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/questions\/alternative-author-rights-policies\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["22304047"],"award-info":[{"award-number":["22304047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Methods Appl. Fluoresc."],"published-print":{"date-parts":[[2026,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mercury ions (Hg\n                    <jats:sup>2+<\/jats:sup>\n                    ) are highly toxic and persistent heavy metal pollutants that pose serious threats to environmental systems and human health. Therefore, developing sensitive and selective detection strategies for Hg\n                    <jats:sup>2+<\/jats:sup>\n                    is of great significance. In this study, a rhodamine-based dual-mode fluorescent probe (RB-HG) was developed utilizing dimethylthiocarbamate groups as recognition sites.\n                    <jats:italic>\n                      Upon undergoing specific interaction with Hg\n                      <jats:sup>2+<\/jats:sup>\n                      , leading to the dissociation of the C\u2013O bond and subsequently triggering an opening-ring reaction to form a ketone structure\n                    <\/jats:italic>\n                    (\n                    <jats:italic>RB-C=O<\/jats:italic>\n                    )\n                    <jats:italic>, thereby activating the intramolecular charge transfer<\/jats:italic>\n                    (\n                    <jats:italic>ICT<\/jats:italic>\n                    )\n                    <jats:italic>process and generating both fluorescence and colorimetric signals,<\/jats:italic>\n                    thereby activating both colorimetric and fluorescence dual\u2014channel signals.\n                    <jats:italic>In vitro<\/jats:italic>\n                    experiments demonstrate that RB-HG (5.0\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M) exhibits high selectivity and sensitivity toward Hg\n                    <jats:sup>2+<\/jats:sup>\n                    . The limits of detection for the fluorescence and colorimetric detection modes are 1.16\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M and 3.22\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M, respectively. RB-HG was successfully applied to detect Hg\n                    <jats:sup>2+<\/jats:sup>\n                    in food samples with excellent recovery rates and to image exogenous Hg\n                    <jats:sup>2+<\/jats:sup>\n                    in living cells, highlighting its potential for food monitoring and bioimaging applications.\n                  <\/jats:p>","DOI":"10.1088\/2050-6120\/ae9f16","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:53:16Z","timestamp":1787784796000},"page":"045003","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel rhodamine-based dual-mode fluorogenic probe for sensitive determination of Hg\u00b2\n                    <sup>+<\/sup>"],"prefix":"10.1088","volume":"14","author":[{"given":"Zhaosheng","family":"Liu","sequence":"first","affiliation":[{"name":"Hengyang Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqin","family":"Wang","sequence":"additional","affiliation":[{"name":"Hengyang Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanfang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Hengyang Ecological and Environmental Affairs Center, Hengyang Municipal Bureau of Ecology and Environment"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hengyang Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Hengyang Ecological and Environmental Affairs Center, Hengyang Municipal Bureau of Ecology and Environment"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Hengyang Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Hengyang Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4668-6322","authenticated-orcid":true,"given":"Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Hengyang Normal University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"mafae9f16bib1","doi-asserted-by":"publisher","first-page":"12002","DOI":"10.1021\/acs.analchem.0c02509","type":"journal-article","article-title":"Imaging Hg2+-induced oxidative stress by NIR molecular probe with \u201cdual-key-and-lock\u201d strategy","volume":"92","author":"Yang","year":"2020","journal-title":"Anal. Chem."},{"key":"mafae9f16bib2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2020.127734","type":"journal-article","article-title":"First fluorescence sensor for simultaneously detecting three kinds of IIB elements (Zn2+, Cd2+ and Hg2+) based on aggregation-induced emission","volume":"308","author":"Jiang","year":"2020","journal-title":"Sens. Actuators B"},{"key":"mafae9f16bib3","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1016\/j.snb.2018.04.011","type":"journal-article","article-title":"A highly specific and ultrasensitive fluorescent probe for monitoring hypochlorous acid and its applications in live cells","volume":"267","author":"Zhu","year":"2018","journal-title":"Sens. Actuators B"},{"key":"mafae9f16bib4","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1016\/j.bios.2015.12.056","type":"journal-article","article-title":"Functionalized gold nanoparticles\/reduced graphene oxide nanocomposites for ultrasensitive electrochemical sensing of mercury ions based on thymine\u2013mercury\u2013thymine structure","volume":"79","author":"Wang","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"mafae9f16bib5","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1016\/j.cej.2017.06.062","type":"journal-article","article-title":"Detection of Hg2+ in aqueous solution by pyrazole derivative-functionalized Fe3O4@SiO2 fluorescent probe","volume":"327","author":"Mir","year":"2017","journal-title":"Chem. Eng. J."},{"key":"mafae9f16bib6","doi-asserted-by":"publisher","first-page":"12052","DOI":"10.1021\/acs.inorgchem.6b02233","type":"journal-article","article-title":"A switch-on NIR probe for specific detection of Hg2+ ion in aqueous medium and in mitochondria","volume":"55","author":"Agarwalla","year":"2016","journal-title":"Inorg. Chem."},{"key":"mafae9f16bib7","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1021\/acssensors.8b00776","type":"journal-article","article-title":"Turn-on fluorescence probe for nitric oxide detection and bioimaging in live cells and zebrafish","volume":"4","author":"Han","year":"2018","journal-title":"ACS Sens."},{"key":"mafae9f16bib8","doi-asserted-by":"publisher","first-page":"3026","DOI":"10.1039\/D3AY00544E","type":"journal-article","article-title":"A fluorescent probe based on an enhanced ICT effect for Hg2+ detection and cell imaging","volume":"15","author":"Pei","year":"2023","journal-title":"Anal. Methods"},{"key":"mafae9f16bib9","doi-asserted-by":"publisher","first-page":"14743","DOI":"10.1021\/acsami.5b02603","type":"journal-article","article-title":"Thymine functionalized graphene oxide for fluorescence \u201cturn-off-on\u201d sensing of Hg2+ and I\u2013in aqueous medium","volume":"7","author":"Dinda","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"mafae9f16bib10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2023.108237","type":"journal-article","article-title":"A new aggregation-induced emission-based fluorescent probe for effective detection of Hg2+ and its multiple applications","volume":"34","author":"Pan","year":"2023","journal-title":"Chin. Chem. Lett."},{"key":"mafae9f16bib11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2023.132999","type":"journal-article","article-title":"Fine-tuning bromide AIE probes for Hg2+ detection in mitochondria with wash-free staining","volume":"464","author":"Tan","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"mafae9f16bib12","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202302809","type":"journal-article","article-title":"All\u2010in\u2010one detection, removal and recovery of Hg2+ in industrial wastewater with plasmonic schottky heterostructures","volume":"33","author":"Wang","year":"2023","journal-title":"Adv. Funct. Mater."},{"key":"mafae9f16bib13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2023.131683","type":"journal-article","article-title":"Controllable synthesis of bifunctional magnetic carbon dots for rapid fluorescent detection and reversible removal of Hg2+","volume":"457","author":"Song","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"mafae9f16bib14","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2022.133979","type":"journal-article","article-title":"A novel Fe3+ fluorescent probe based on rhodamine derivatives and its application in biological imaging","volume":"1270","author":"Wang","year":"2022","journal-title":"J. Mol. Struct."},{"key":"mafae9f16bib15","doi-asserted-by":"publisher","first-page":"12532","DOI":"10.1021\/acs.analchem.6b04392","type":"journal-article","article-title":"Highly specific and ultrasensitive two-photon fluorescence imaging of native HOCl in lysosomes and tissues based on thiocarbamate derivatives","volume":"88","author":"Zhu","year":"2016","journal-title":"Anal. Chem."},{"key":"mafae9f16bib16","doi-asserted-by":"publisher","first-page":"3364","DOI":"10.1039\/D4AY00380B","type":"journal-article","article-title":"Construction of a novel fluorescent probe for sensitive determination of glyphosate in food and imaging living cells","volume":"16","author":"Lin","year":"2024","journal-title":"Anal. Methods"},{"key":"mafae9f16bib17","doi-asserted-by":"publisher","first-page":"4079","DOI":"10.1039\/C8AY01176A","type":"journal-article","article-title":"Reaction-based fluorescent probes for rapid detection of hydrogen sulfide in vivo","volume":"10","author":"Jin","year":"2018","journal-title":"Anal. Methods"},{"key":"mafae9f16bib18","doi-asserted-by":"publisher","first-page":"10554","DOI":"10.1039\/C9RA00622B","type":"journal-article","article-title":"Highly selective and sensitive fluorescent probe for the rapid detection of mercury ions","volume":"9","author":"Xu","year":"2019","journal-title":"RSC Adv."},{"key":"mafae9f16bib19","doi-asserted-by":"publisher","first-page":"8004","DOI":"10.1039\/C5AY01581B","type":"journal-article","article-title":"A guanidinium modified rhodamine-based fluorescent probe for in vitro\/vivo imaging of gold ions","volume":"7","author":"Karaku\u015f","year":"2015","journal-title":"Anal. Methods"},{"key":"mafae9f16bib20","doi-asserted-by":"publisher","first-page":"5554","DOI":"10.1039\/C8AY01887A","type":"journal-article","article-title":"A novel fluorescent probe for Hg2+ detection in a wide pH range and its application in living cell imaging","volume":"10","author":"Xu","year":"2018","journal-title":"Anal. Methods"},{"key":"mafae9f16bib21","doi-asserted-by":"publisher","first-page":"1846","DOI":"10.1039\/D3AY02106H","type":"journal-article","article-title":"A ratiometric fluorescent probe with a large stokes shift for the detection of Hg2+ and its applications in environmental sample and living system analysis","volume":"16","author":"Tian","year":"2024","journal-title":"Anal. Methods"},{"key":"mafae9f16bib22","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1016\/j.snb.2017.04.074","type":"journal-article","article-title":"Hexaphenylbenzene appended AIEE active FRET based fluorescent probe for selective imaging of Hg2+ ions in MCF-7 cell lines","volume":"249","author":"Singh","year":"2017","journal-title":"Sens. Actuators B"},{"key":"mafae9f16bib23","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1039\/C9AY02303H","type":"journal-article","article-title":"The construction of a CND\/Cu2+ fluorescence sensing system for the ultrasensitive detection of glyphosate","volume":"12","author":"Wang","year":"2020","journal-title":"Anal. Methods"},{"key":"mafae9f16bib24","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1016\/j.talanta.2016.07.020","type":"journal-article","article-title":"Facile, green and clean one-step synthesis of carbon dots from wool: application as a sensor for glyphosate detection based on the inner filter effect","volume":"160","author":"Wang","year":"2016","journal-title":"Talanta"},{"key":"mafae9f16bib25","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126815","type":"journal-article","article-title":"Dual lanthanide-probe based on coordination polymer networks for ratiometric detection of glyphosate in food samples","volume":"323","author":"Qu","year":"2020","journal-title":"Food Chem."},{"key":"mafae9f16bib26","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1021\/acssensors.6b00001","type":"journal-article","article-title":"High-performance colorimetric detection of Hg2+ based on triangular silver nanoprisms","volume":"1","author":"Chen","year":"2016","journal-title":"ACS Sens."},{"key":"mafae9f16bib27","doi-asserted-by":"publisher","first-page":"7725","DOI":"10.1039\/D0CS01340D","type":"journal-article","article-title":"Recent progress in fluorescent probes for bacteria","volume":"50","author":"Huang","year":"2021","journal-title":"Chem. Soc. Rev."},{"key":"mafae9f16bib28","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2020.108321","type":"journal-article","article-title":"Recent progress in the development of fluorescent probes for detection of biothiols","volume":"177","author":"Dai","year":"2020","journal-title":"Dyes Pigm."},{"key":"mafae9f16bib29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2020.213638","type":"journal-article","article-title":"Fluorescent probes for biothiols based on metal complex","volume":"429","author":"Liu","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"mafae9f16bib30","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1039\/C9CS00243J","type":"journal-article","article-title":"Synthetic ratiometric fluorescent probes for detection of ions","volume":"49","author":"Park","year":"2020","journal-title":"Chem. Soc. Rev."},{"key":"mafae9f16bib31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2020.213582","type":"journal-article","article-title":"Recent progresses in fluorescent probes for detection of polarity","volume":"427","author":"Xiao","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"mafae9f16bib32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2020.213686","type":"journal-article","article-title":"Advances and perspectives in carbon dot-based fluorescent probes: mechanism, and application","volume":"431","author":"Li","year":"2021","journal-title":"Coord. Chem. Rev."},{"key":"mafae9f16bib33","doi-asserted-by":"publisher","first-page":"9257","DOI":"10.1039\/C9SC02764E","type":"journal-article","article-title":"Engineering an NIR rhodol derivative with spirocyclic ring-opening activation for high-contrast photoacoustic imaging","volume":"10","author":"Liu","year":"2019","journal-title":"Chem. Sci."},{"key":"mafae9f16bib34","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1016\/j.bmc.2010.05.074","type":"journal-article","article-title":"Design and synthesis of a novel fluorescence probe for Zn2+ based on the spirolactam ring-opening process of rhodamine derivatives","volume":"19","author":"Sasaki","year":"2011","journal-title":"Bioorg. Med. Chem."},{"key":"mafae9f16bib35","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2025.138338","type":"journal-article","article-title":"Turn-on fluorescent probe for vicinal dithiol-containing proteins via spirocyclic ring-opening of rhodamine and its application in cell imaging","volume":"444","author":"Shi","year":"2025","journal-title":"Sens. Actuators B"},{"key":"mafae9f16bib36","doi-asserted-by":"publisher","first-page":"1805","DOI":"10.1039\/c3ob27356c","type":"journal-article","article-title":"An acid catalyzed reversible ring-opening\/ring-closure reaction involving a cyano-rhodamine spirolactam","volume":"11","author":"Li","year":"2013","journal-title":"Org. Biomol. Chem."},{"key":"mafae9f16bib37","doi-asserted-by":"publisher","first-page":"6110","DOI":"10.1039\/c2dt32198j","type":"journal-article","article-title":"Discrimination of fluorescence light-up effects induced by pH and metal ion chelation on a spirocyclic derivative of rhodamine B","volume":"42","author":"Leite","year":"2013","journal-title":"Dalton Trans."},{"key":"mafae9f16bib38","doi-asserted-by":"publisher","first-page":"8167","DOI":"10.1021\/acsomega.7b01324","type":"journal-article","article-title":"New six-membered pH-insensitive rhodamine spirocycle in selective sensing of Cu2+ through C\u2013C bond cleavage and its application in cell imaging","volume":"2","author":"Majumdar","year":"2017","journal-title":"ACS Omega"},{"key":"mafae9f16bib39","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.dyepig.2012.03.008","type":"journal-article","article-title":"A fluorescent sensor for pH based on rhodamine fluorophore","volume":"95","author":"Tian","year":"2012","journal-title":"Dyes Pigm."},{"key":"mafae9f16bib40","doi-asserted-by":"publisher","DOI":"10.1002\/qua.25780","type":"journal-article","article-title":"Why the lowest electronic excitations of rhodamines are overestimated by time\u2010dependent density functional theory","volume":"118","author":"Zhou","year":"2018","journal-title":"Int. J. Quantum Chem."},{"key":"mafae9f16bib41","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochem.2025.116986","type":"journal-article","article-title":"A novel AIE fluorescent probe for sensitive determination of glyphosate in food and imaging living cells","volume":"15","author":"Lin","year":"2025","journal-title":"J. Photochem. Photobiol. A"}],"container-title":["Methods and Applications in Fluorescence"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:51:14Z","timestamp":1788353474000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/ae9f16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":41,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,9,2]]},"published-print":{"date-parts":[[2026,12,1]]}},"URL":"https:\/\/doi.org\/10.1088\/2050-6120\/ae9f16","relation":{},"ISSN":["2050-6120"],"issn-type":[{"value":"2050-6120","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]},"assertion":[{"value":"A novel rhodamine-based dual-mode fluorogenic probe for sensitive determination of Hg\u00b2\n                      +","name":"article_title","label":"Article Title"},{"value":"Methods and Applications in Fluorescence","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2026-05-28","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-08-26","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-09-02","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}