{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:40:46Z","timestamp":1793727646664,"version":"4.6.1"},"reference-count":171,"publisher":"IOP Publishing","issue":"4","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"Engineering and Physical Sciences Research Council","award":["EP\/I003983\/1"],"award-info":[{"award-number":["EP\/I003983\/1"]}],"id":[{"id":"https:\/\/ror.org\/0439y7842","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Methods Appl. Fluoresc."],"published-print":{"date-parts":[[2026,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lipid packing in cellular membranes exerts direct effect on membrane tension and microviscosity, and plays a central role in cellular adaptation, homeostasis and disease development. According to conventional mechanical descriptions, viscosity and tension are directly interconnected, with increased tension leading to decreased membrane microviscosity. However, intricate molecular interactions that contribute to provide the structure and function of cellular membranes suggest a more complex relationship between these parameters. In this perspective, we review recent progress in using environmentally sensitive fluorophores to map changes in lipid structure, corresponding to alterations in the bilayer hydration, polarity, microviscosity or tension. We focus particularly on the use of fluorescence lifetime imaging microscopy that enables quantitative concentration-independent measurements of these parameters.<\/jats:p>","DOI":"10.1088\/2050-6120\/aeaea2","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:56:09Z","timestamp":1790808969000},"page":"043101","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Recent developments in fluorescence lifetime imaging of lipid membrane dynamics"],"prefix":"10.1088","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3933-8722","authenticated-orcid":false,"given":"Miguel","family":"Paez-Perez","sequence":"first","affiliation":[{"name":"Imperial College London"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8529-7378","authenticated-orcid":true,"given":"Maya","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Imperial College London"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2383-6014","authenticated-orcid":true,"given":"Marina K","family":"Kuimova","sequence":"additional","affiliation":[{"name":"Imperial College London"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"266","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"mafaeaea2bib1","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1038\/s41580-022-00524-4","type":"journal-article","article-title":"Regulation of membrane protein structure and function by their lipid nano-environment","volume":"24","author":"Levental","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"mafaeaea2bib2","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1038\/nrm2330","type":"journal-article","article-title":"Membrane lipids: where they are and how they behave","volume":"9","author":"Van Meer","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"mafaeaea2bib3","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1038\/s41580-022-00511-9","type":"journal-article","article-title":"Generation of nanoscopic membrane curvature for membrane trafficking","volume":"24","author":"Kozlov","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"mafaeaea2bib4","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1038\/nrm.2017.138","type":"journal-article","article-title":"Understanding the diversity of membrane lipid composition","volume":"19","author":"Harayama","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"mafaeaea2bib5","doi-asserted-by":"publisher","first-page":"5849","DOI":"10.1021\/acs.chemrev.8b00439","type":"journal-article","article-title":"Biological membrane organization and cellular signaling","volume":"119","author":"Cheng","year":"2019","journal-title":"Chem. Rev."},{"key":"mafaeaea2bib6","doi-asserted-by":"publisher","first-page":"2231","DOI":"10.1016\/j.bbamem.2016.06.025","type":"journal-article","article-title":"Biophysics in cancer: the relevance of drug-membrane interaction studies","volume":"1858","author":"Alves","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"mafaeaea2bib7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2412914121","type":"journal-article","article-title":"Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells","volume":"121","author":"Matsuzaki","year":"2024","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"mafaeaea2bib8","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1002\/med.21868","type":"journal-article","article-title":"Lipidomic landscape in cancer: actionable insights for membrane-based therapy and diagnoses","volume":"42","author":"Agarwala","year":"2022","journal-title":"Med. Res. Rev."},{"key":"mafaeaea2bib9","doi-asserted-by":"publisher","first-page":"3620","DOI":"10.1038\/s41467-021-23626-7","type":"journal-article","article-title":"4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation","volume":"12","author":"Geoghegan","year":"2021","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib10","doi-asserted-by":"publisher","first-page":"4545","DOI":"10.1007\/s00018-021-03799-6","type":"journal-article","article-title":"Of membranes and malaria: phospholipid asymmetry in Plasmodium falciparum-infected red blood cells","volume":"78","author":"Fraser","year":"2021","journal-title":"Cell Mol. Life Sci."},{"key":"mafaeaea2bib11","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2020.101049","type":"journal-article","article-title":"Brain lipid peroxidation and Alzheimer disease: synergy between the Butterfield and Mattson laboratories","volume":"64","author":"Butterfield","year":"2020","journal-title":"Ageing Res. Rev."},{"key":"mafaeaea2bib12","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.cca.2019.07.037","type":"journal-article","article-title":"Lipid peroxidation in neurodegeneration","volume":"497","author":"Pe\u00f1a-Bautista","year":"2019","journal-title":"Clin. Chim. Acta"},{"key":"mafaeaea2bib13","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1097\/MOL.0b013e3282fe1d0e","type":"journal-article","article-title":"Oxidized phospholipids: emerging lipid mediators in pathophysiology","volume":"19","author":"Deigner","year":"2008","journal-title":"Curr. Opin. Lipidol."},{"key":"mafaeaea2bib14","doi-asserted-by":"publisher","first-page":"7634","DOI":"10.3390\/ijms22147634","type":"journal-article","article-title":"Molecular mechanisms of lipid metabolism disorders in infectious exacerbations of chronic obstructive pulmonary disease","volume":"22","author":"Kotlyarov","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"mafaeaea2bib15","doi-asserted-by":"publisher","first-page":"4637","DOI":"10.1021\/acsnano.4c14927","type":"journal-article","article-title":"Nanoscale viscometry reveals an inherent mucus defect in cystic fibrosis","volume":"19","author":"Ponomarchuk","year":"2025","journal-title":"ACS Nano"},{"key":"mafaeaea2bib16","doi-asserted-by":"publisher","first-page":"S207","DOI":"10.1194\/jlr.R800074-JLR200","type":"journal-article","article-title":"The role of oxidized phospholipids in atherosclerosis","volume":"50","author":"Berliner","year":"2009","journal-title":"J. Lipid Res."},{"key":"mafaeaea2bib17","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1038\/s41586-018-0198-8","type":"journal-article","article-title":"Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice","volume":"558","author":"Que","year":"2018","journal-title":"Nature"},{"key":"mafaeaea2bib18","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.1038\/s41565-026-02236-8","type":"journal-article","article-title":"Spectral biophysical cytometry with nanosensors reveals remodelling of immune cells in atherosclerosis","volume":"21","author":"Gurdap","year":"2026","journal-title":"Nat. Nanotechnol."},{"key":"mafaeaea2bib19","doi-asserted-by":"publisher","first-page":"H1178","DOI":"10.1152\/ajpheart.00241.2015","type":"journal-article","article-title":"Vascular endothelial cell membranes differentiate between stretch and shear stress through transitions in their lipid phases","volume":"309","author":"Yamamoto","year":"2015","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"mafaeaea2bib20","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1039\/d0nh00605j","type":"journal-article","article-title":"Targeted liposomal drug delivery: a nanoscience and biophysical perspective","volume":"6","author":"Liu","year":"2021","journal-title":"Nanoscale Horiz."},{"key":"mafaeaea2bib21","doi-asserted-by":"publisher","first-page":"5134","DOI":"10.1021\/acs.langmuir.0c00262","type":"journal-article","article-title":"Membrane fluidity as a new means to selectively target cancer cells with fusogenic lipid carriers","volume":"36","author":"Bompard","year":"2020","journal-title":"Langmuir"},{"key":"mafaeaea2bib22","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202408039","type":"journal-article","article-title":"Membrane fusion\u2010based drug delivery liposomes transiently modify the material properties of synthetic and biological membranes","volume":"21","author":"Hammond","year":"2025","journal-title":"Small"},{"key":"mafaeaea2bib23","doi-asserted-by":"publisher","first-page":"9389","DOI":"10.1021\/acsnano.2c02195","type":"journal-article","article-title":"Dynamic reconfiguration of subcompartment architectures in artificial cells","volume":"16","author":"Zubaite","year":"2022","journal-title":"ACS Nano"},{"key":"mafaeaea2bib24","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202500928","type":"journal-article","article-title":"Ion- and temperature-programmable reconfiguration of subcompartments in synthetic cells","volume":"27","author":"Xu","year":"2026","journal-title":"ChemBioChem"},{"key":"mafaeaea2bib25","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/s41467-024-53975-y","type":"journal-article","article-title":"A tuneable minimal cell membrane reveals that two lipid species suffice for life","volume":"15","author":"Justice","year":"2024","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib26","doi-asserted-by":"publisher","first-page":"3842","DOI":"10.1038\/s41467-025-59260-w","type":"journal-article","article-title":"Local mapping of the nanoscale viscoelastic properties of fluid membranes by AFM nanorheology","volume":"16","author":"Trewby","year":"2025","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib27","doi-asserted-by":"publisher","first-page":"15263","DOI":"10.1021\/acs.jpcb.5b06604","type":"journal-article","article-title":"Effects of lipid composition on bilayer membranes quantified by all-atom molecular dynamics","volume":"119","author":"Ding","year":"2015","journal-title":"J. Phys. Chem. B"},{"key":"mafaeaea2bib28","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/S0304-4157(96)00009-3","type":"journal-article","article-title":"Lateral pressure in membranes","volume":"1286","author":"Marsh","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"mafaeaea2bib29","first-page":"41","type":"journal-article","article-title":"Membranes using Di-Pyrenyl phosphatidylcholine","volume":"111","author":"Templer","year":"1998","journal-title":"Distribution"},{"key":"mafaeaea2bib30","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1038\/s41589-020-00688-0","type":"journal-article","article-title":"Structural and functional consequences of reversible lipid asymmetry in living membranes","volume":"16","author":"Doktorova","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"mafaeaea2bib31","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1016\/j.bpj.2019.11.3398","type":"journal-article","article-title":"Spontaneous curvature, differential stress, and bending modulus of asymmetric lipid membranes","volume":"118","author":"Hossein","year":"2020","journal-title":"Biophys. J."},{"key":"mafaeaea2bib32","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1128\/mcb.26.1.313-323.2006","type":"journal-article","article-title":"Identifying optimal lipid raft characteristics required to promote nanoscale protein-protein interactions on the plasma membrane","volume":"26","author":"Nicolau","year":"2006","journal-title":"Mol. Cell Biol."},{"key":"mafaeaea2bib33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcell.2021.706731","type":"journal-article","article-title":"Receptor\/Raft Ratio Is a Determinant for LRP6 Phosphorylation and WNT\/\u03b2-catenin signaling","volume":"9","author":"Haack","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"mafaeaea2bib34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1103\/PhysRevE.95.012403","type":"journal-article","article-title":"Impact of lipid rafts on the T -cell-receptor and peptide-major-histocompatibility-complex interactions under different measurement conditions","volume":"95","author":"Li","year":"2017","journal-title":"Phys. Rev. E"},{"key":"mafaeaea2bib35","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1038\/35036052","type":"journal-article","article-title":"Lipid rafts and signal transduction","volume":"1","author":"Simons","year":"2000","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"mafaeaea2bib36","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2019.183091","type":"journal-article","article-title":"Disruption of palmitate-mediated localization; a shared pathway of force and anesthetic activation of TREK-1 channels","volume":"1862","author":"Petersen","year":"2020","journal-title":"Biochim. Biophys. Acta"},{"key":"mafaeaea2bib37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/ncomms13873","type":"journal-article","article-title":"Kinetic disruption of lipid rafts is a mechanosensor for phospholipase D","volume":"7","author":"Petersen","year":"2016","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib38","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S0304-4157(98)00006-9","type":"journal-article","article-title":"Phases and phase transitions of the phosphatidylcholines","volume":"1376","author":"Koynova","year":"1998","journal-title":"Biochim. Biophys. Acta"},{"key":"mafaeaea2bib39","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/S0006-3495(89)82807-3","type":"journal-article","article-title":"Structure of the fully hydrated gel phase of dipalmitoylphosphatidylcholine","volume":"55","author":"Wiener","year":"1989","journal-title":"Biophys. J."},{"key":"mafaeaea2bib40","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.chemphyslip.2018.12.011","type":"journal-article","article-title":"Structure of gel phase DPPC determined by x-ray diffraction","volume":"218","author":"Nagle","year":"2019","journal-title":"Chem. Phys. Lipids"},{"key":"mafaeaea2bib41","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.chemphyslip.2011.11.001","type":"journal-article","article-title":"Lateral order in gel, subgel and crystalline phases of lipid membranes: wide-angle x-ray scattering","volume":"165","author":"Marsh","year":"2012","journal-title":"Chem. Phys. Lipids"},{"key":"mafaeaea2bib42","doi-asserted-by":"publisher","first-page":"5779","DOI":"10.1039\/c2nr31465g","type":"journal-article","article-title":"Lipid crystallization: from self-assembly to hierarchical and biological ordering","volume":"4","author":"Kulkarni","year":"2012","journal-title":"Nanoscale"},{"key":"mafaeaea2bib43","doi-asserted-by":"publisher","first-page":"12006","DOI":"10.1021\/acs.analchem.3c01747","type":"journal-article","article-title":"Viscosity-sensitive membrane dyes as tools to estimate the crystalline structure of lipid bilayers","volume":"95","author":"Paez-Perez","year":"2023","journal-title":"Anal. Chem."},{"key":"mafaeaea2bib44","doi-asserted-by":"publisher","first-page":"2914","DOI":"10.1016\/j.bbamem.2007.08.012","type":"journal-article","article-title":"Bilayer polarity and its thermal dependency in the \u2113o and \u2113d phases of binary phosphatidylcholine\/cholesterol mixtures","volume":"1768","author":"Arrais","year":"2007","journal-title":"Biochim. Biophys."},{"key":"mafaeaea2bib45","doi-asserted-by":"publisher","first-page":"9055","DOI":"10.1039\/c5sm01807b","type":"journal-article","article-title":"On scattered waves and lipid domains: detecting membrane rafts with x-rays and neutrons","volume":"11","author":"Marquardt","year":"2015","journal-title":"Soft Matter"},{"key":"mafaeaea2bib46","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1016\/j.bpj.2019.01.004","type":"journal-article","article-title":"Biophysical perspective advances in micropipette aspiration: applications in cell biomechanics, models, and extended studies","volume":"116","author":"Gonz\u00e1lez-Berm\u00fadez","year":"2019","journal-title":"Biophys. J."},{"key":"mafaeaea2bib47","doi-asserted-by":"publisher","first-page":"6286","DOI":"10.1039\/d5na00702j","type":"journal-article","article-title":"Advances in nanomechanical property mapping by atomic force microscopy","volume":"7","author":"Garcia","year":"2025","journal-title":"Nanoscale Adv."},{"key":"mafaeaea2bib48","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.bbamem.2009.12.019","type":"journal-article","article-title":"Nanomechanics of lipid bilayers by force spectroscopy with AFM : a perspective","volume":"1798","author":"Garcia-Manyes","year":"2010","journal-title":"Biochim. Biophys. Acta"},{"key":"mafaeaea2bib49","doi-asserted-by":"publisher","first-page":"8996","DOI":"10.1039\/d0sm00943a","type":"journal-article","article-title":"Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy","volume":"16","author":"Faizi","year":"2020","journal-title":"Soft Matter"},{"key":"mafaeaea2bib50","doi-asserted-by":"publisher","first-page":"7385","DOI":"10.1039\/c5sm01565k","type":"journal-article","article-title":"Viscoelastic deformation of lipid bilayer vesicles","volume":"11","author":"Wu","year":"2015","journal-title":"Soft Matter"},{"key":"mafaeaea2bib51","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1021\/acsmacrolett.8b00498","type":"journal-article","article-title":"Optical tweezers microrheology: from the basics to advanced techniques and applications","volume":"7","author":"Robertson-Anderson","year":"2018","journal-title":"ACS Macro Lett."},{"key":"mafaeaea2bib52","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1038\/s43586-023-00286-z","type":"journal-article","article-title":"Brillouin microscopy","volume":"4","author":"Kabakova","year":"2024","journal-title":"Nat. Rev. Methods Primers"},{"key":"mafaeaea2bib53","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1038\/s41592-019-0543-3","type":"journal-article","article-title":"Brillouin microscopy: an emerging tool for mechanobiology","volume":"16","author":"Prevedel","year":"2019","journal-title":"Nat. Methods"},{"key":"mafaeaea2bib54","doi-asserted-by":"publisher","first-page":"7833","DOI":"10.1021\/acs.chemrev.9b00019","type":"journal-article","article-title":"Brillouin light scattering: applications in biomedical sciences","volume":"119","author":"Palombo","year":"2019","journal-title":"Chem. Rev."},{"key":"mafaeaea2bib55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphy.2021.644450","type":"journal-article","article-title":"A comprehensive review of fluorescence correlation spectroscopy","volume":"9","author":"Yu","year":"2021","journal-title":"Front. Phys."},{"key":"mafaeaea2bib56","doi-asserted-by":"publisher","first-page":"7331","DOI":"10.1021\/acs.chemrev.6b00815","type":"journal-article","article-title":"Single particle tracking: from theory to biophysical applications","volume":"117","author":"Shen","year":"2017","journal-title":"Chem. Rev."},{"key":"mafaeaea2bib57","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1017\/S0033583515000013","type":"journal-article","article-title":"Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice","volume":"48","author":"Lor\u00e9n","year":"2015","journal-title":"Q. Rev. Biophys."},{"key":"mafaeaea2bib58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1021\/acs.accounts.2c00586","type":"journal-article","article-title":"Fluorescent probes for lipid membranes: from the cell surface to organelles","volume":"56","author":"Klymchenko","year":"2023","journal-title":"Acc. Chem. Res."},{"key":"mafaeaea2bib59","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202217868","type":"journal-article","article-title":"Fluorescent flippers: small\u2010molecule probes to image membrane tension in living systems","volume":"62","author":"Chen","year":"2023","journal-title":"Angew. Chem., Int. Ed."},{"key":"mafaeaea2bib60","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202311233","type":"journal-article","article-title":"Molecular rotors: fluorescent sensors for microviscosity and conformation of biomolecules","volume":"63","author":"Paez\u2010Perez","year":"2024","journal-title":"Angew. Chem., Int. Ed."},{"key":"mafaeaea2bib61","doi-asserted-by":"publisher","first-page":"5106","DOI":"10.1021\/acs.langmuir.3c02941","type":"journal-article","article-title":"Exploring the physical properties of lipid membranes with polyhydroxy oxanorbornane head group using NBD-conjugated and DPH fluorescent probes","volume":"40","author":"Sahu","year":"2024","journal-title":"Langmuir"},{"key":"mafaeaea2bib62","doi-asserted-by":"publisher","first-page":"2154","DOI":"10.1021\/acs.jpcb.3c07006","type":"journal-article","article-title":"Quantifying fluorescence lifetime responsiveness of environment-sensitive probes for membrane fluidity measurements","volume":"128","author":"Ragaller","year":"2024","journal-title":"J. Phys. Chem. B"},{"key":"mafaeaea2bib63","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphy.2021.648553","type":"journal-article","article-title":"A review of new high-throughput methods designed for fluorescence lifetime sensing from cells and tissues","volume":"9","author":"Bitton","year":"2021","journal-title":"Front. Phys."},{"key":"mafaeaea2bib64","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1117\/1.1586704","type":"journal-article","article-title":"Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods","volume":"8","author":"Gratton","year":"2003","journal-title":"J. Biomed. Opt."},{"key":"mafaeaea2bib65","doi-asserted-by":"publisher","DOI":"10.1039\/c3cp51953h","type":"journal-article","article-title":"Molecular rheometry: direct determination of viscosity in Lo and Ld lipid phases via fluorescence lifetime imaging","volume":"15","author":"Wu","year":"2013","journal-title":"Phys. Chem. Chem. Phys."},{"key":"mafaeaea2bib66","doi-asserted-by":"publisher","first-page":"18393","DOI":"10.1039\/C5CP01937K","type":"journal-article","article-title":"Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors","volume":"17","author":"Dent","year":"2015","journal-title":"Phys. Chem. Chem. Phys."},{"key":"mafaeaea2bib67","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1021\/acs.accounts.6b00517","type":"journal-article","article-title":"Solvatochromic and fluorogenic dyes as environment-sensitive probes: design and biological applications","volume":"50","author":"Klymchenko","year":"2017","journal-title":"Acc. Chem. Res."},{"key":"mafaeaea2bib68","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1021\/acs.accounts.0c00687","type":"journal-article","article-title":"LAURDAN since weber: the quest for visualizing membrane heterogeneity","volume":"54","author":"Gunther","year":"2021","journal-title":"Acc. Chem. Res."},{"key":"mafaeaea2bib69","doi-asserted-by":"publisher","first-page":"2563","DOI":"10.1529\/biophysj.105.072884","type":"journal-article","article-title":"Characterization and application of a new optical probe for membrane lipid domains","volume":"90","author":"Jin","year":"2006","journal-title":"Biophys. J."},{"key":"mafaeaea2bib70","doi-asserted-by":"publisher","first-page":"6570","DOI":"10.1039\/d4tb00872c","type":"journal-article","article-title":"Sensing cholesterol-induced rigidity in model membranes with time-resolved fluorescence spectroscopy and microscopy","volume":"12","author":"Biswas","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"mafaeaea2bib71","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.jphotochem.2019.03.006","type":"journal-article","article-title":"A closer look into Laurdan as a probe to monitor cationic DODAB bilayers","volume":"376","author":"Masukawa","year":"2019","journal-title":"J. Photochem. Photobiol. A"},{"key":"mafaeaea2bib72","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1016\/j.bpj.2012.12.057","type":"journal-article","article-title":"Laurdan fluorescence lifetime discriminates cholesterol content from changes in fluidity in living cell membranes","volume":"104","author":"Golfetto","year":"2013","journal-title":"Biophys. J."},{"key":"mafaeaea2bib73","doi-asserted-by":"publisher","first-page":"1498","DOI":"10.1016\/j.bpj.2018.08.041","type":"journal-article","article-title":"Time-resolved Laurdan fluorescence reveals insights into membrane viscosity and hydration levels","volume":"115","author":"Ma","year":"2018","journal-title":"Biophys. J."},{"key":"mafaeaea2bib74","doi-asserted-by":"publisher","first-page":"L80","DOI":"10.1529\/biophysj.106.084673","type":"journal-article","article-title":"Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells","volume":"90","author":"Owen","year":"2006","journal-title":"Biophys. J."},{"key":"mafaeaea2bib75","doi-asserted-by":"publisher","first-page":"1374","DOI":"10.1021\/jacs.3c10132","type":"journal-article","article-title":"Lipid peroxidation drives liquid-liquid phase separation and disrupts raft protein partitioning in biological membranes","volume":"146","author":"Balakrishnan","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib76","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1038\/s41557-018-0127-3","type":"journal-article","article-title":"A fluorescent membrane tension probe","volume":"10","author":"Colom","year":"2018","journal-title":"Nat. Chem."},{"key":"mafaeaea2bib77","doi-asserted-by":"publisher","first-page":"24114","DOI":"10.1021\/jacs.4c08580","type":"journal-article","article-title":"Tensing flipper: photosensitized manipulation of membrane tension, lipid phase separation, and raft protein sorting in biological membranes","volume":"146","author":"Torra","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib78","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/0009-2614(80)80574-4","type":"journal-article","article-title":"Fluorescence probe for microenvironments: anomalous viscosity dependence of the fluorescence quantum yield of p-N,N-dialkylaminobenzylidenemalononitrile in 1-alkanols","volume":"75","author":"Law","year":"1980","journal-title":"Chem. Phys. Lett."},{"key":"mafaeaea2bib79","doi-asserted-by":"publisher","first-page":"4205","DOI":"10.1021\/j100218a023","type":"journal-article","article-title":"Effect of viscosity and temperature on torsional relaxation of molecular rotors","volume":"86","author":"Loutfy","year":"1982","journal-title":"J. Phys. Chem."},{"key":"mafaeaea2bib80","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/1754-1611-4-11","type":"journal-article","article-title":"Environment-sensitive behavior of fluorescent molecular rotors","volume":"4","author":"Haidekker","year":"2010","journal-title":"J. Biol. Eng."},{"key":"mafaeaea2bib81","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/j.bioorg.2005.07.005","type":"journal-article","article-title":"Effects of solvent polarity and solvent viscosity on the fluorescent properties of molecular rotors and related probes","volume":"33","author":"Haidekker","year":"2005","journal-title":"Bioorg. Chem."},{"key":"mafaeaea2bib82","doi-asserted-by":"publisher","first-page":"2414","DOI":"10.1021\/acs.jpclett.9b00668","type":"journal-article","article-title":"Solvatochromic near-infrared probe for polarity mapping of biomembranes and lipid droplets in cells under stress","volume":"10","author":"Ashoka","year":"2019","journal-title":"J. Phys. Chem. Lett."},{"key":"mafaeaea2bib83","doi-asserted-by":"publisher","first-page":"5773","DOI":"10.1039\/C5SC02248G","type":"journal-article","article-title":"Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors","volume":"6","author":"Vy\u0161niauskas","year":"2015","journal-title":"Chem. Sci."},{"key":"mafaeaea2bib84","doi-asserted-by":"publisher","first-page":"1669","DOI":"10.1039\/B618415D","type":"journal-article","article-title":"Molecular rotors\u2014fluorescent biosensors for viscosity and flow","volume":"5","author":"Haidekker","year":"2007","journal-title":"Org. Biomol. Chem."},{"key":"mafaeaea2bib85","doi-asserted-by":"publisher","first-page":"6672","DOI":"10.1021\/ja800570d","type":"journal-article","article-title":"Molecular rotor measures viscosity of live cells via fluorescence lifetime imaging","volume":"130","author":"Kuimova","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib86","doi-asserted-by":"publisher","first-page":"5282","DOI":"10.1039\/C3CC47530A","type":"journal-article","article-title":"A molecular rotor for measuring viscosity in plasma membranes of live cells","volume":"50","author":"L\u00f3pez-Duarte","year":"2014","journal-title":"Chem. Commun."},{"key":"mafaeaea2bib87","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/s42003-019-0583-3","type":"journal-article","article-title":"Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density","volume":"2","author":"Steink\u00fchler","year":"2019","journal-title":"Commun. Biol."},{"key":"mafaeaea2bib88","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1016\/j.bpj.2019.01.030","type":"journal-article","article-title":"Solvatochromic modeling of Laurdan for multiple polarity analysis of dihydrosphingomyelin bilayer","volume":"116","author":"Watanabe","year":"2019","journal-title":"Biophys. J."},{"key":"mafaeaea2bib89","doi-asserted-by":"publisher","DOI":"10.1117\/1.jbo.20.9.096002","type":"journal-article","article-title":"Spectrally resolved fluorescence lifetime imaging of Nile red for measurements of intracellular polarity","volume":"20","author":"Levitt","year":"2015","journal-title":"J. Biomed. Opt."},{"key":"mafaeaea2bib90","doi-asserted-by":"publisher","first-page":"25252","DOI":"10.1039\/c7cp03571c","type":"journal-article","article-title":"Exploring viscosity, polarity and temperature sensitivity of BODIPY-based molecular rotors","volume":"19","author":"Vy\u0161niauskas","year":"2017","journal-title":"Phys. Chem. Chem. Phys."},{"key":"mafaeaea2bib91","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.biomaterials.2017.06.009","type":"journal-article","article-title":"Probing supramolecular protein assembly using covalently attached fluorescent molecular rotors","volume":"139","author":"Kub\u00e1nkov\u00e1","year":"2017","journal-title":"Biomaterials"},{"key":"mafaeaea2bib92","doi-asserted-by":"publisher","first-page":"2604","DOI":"10.1039\/D0SC05874B","type":"journal-article","article-title":"Imaging non-classical mechanical responses of lipid membranes using molecular rotors","volume":"12","author":"P\u00e1ez-P\u00e9rez","year":"2021","journal-title":"Chem. Sci."},{"key":"mafaeaea2bib93","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1524\/zpch.1971.75.1_2.063","type":"journal-article","article-title":"Viscosity dependence of fluorescent quantum yields of some dye systems","volume":"75","author":"F\u00f6rster","year":"1971","journal-title":"Z. Phys. Chem."},{"key":"mafaeaea2bib94","doi-asserted-by":"publisher","first-page":"8296","DOI":"10.1039\/C9CP06865A","type":"journal-article","article-title":"The effect of solvent polarity and macromolecular crowding on the viscosity sensitivity of a molecular rotor BODIPY-C 10","volume":"22","author":"Polita","year":"2020","journal-title":"Phys. Chem. Chem. Phys."},{"key":"mafaeaea2bib95","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2022.11.1332","type":"journal-article","article-title":"Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties","volume":"12","author":"Ragaller","year":"2022","journal-title":"Open Biol."},{"key":"mafaeaea2bib96","doi-asserted-by":"publisher","first-page":"1592","DOI":"10.1016\/j.bpj.2024.04.033","type":"journal-article","article-title":"Fluorescence lifetime imaging microscopy of flexible and rigid dyes probes the biophysical properties of synthetic and biological membranes","volume":"123","author":"Lira","year":"2024","journal-title":"Biophys. J."},{"key":"mafaeaea2bib97","doi-asserted-by":"publisher","first-page":"13984","DOI":"10.1073\/pnas.1102125108","type":"journal-article","article-title":"Sensing membrane stress with near IR-emissive porphyrins","volume":"108","author":"Kamat","year":"2011","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"mafaeaea2bib98","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/s42004-022-00809-x","type":"journal-article","article-title":"Directly imaging emergence of phase separation in peroxidized lipid membranes","volume":"6","author":"Paez-Perez","year":"2023","journal-title":"Commun. Chem."},{"key":"mafaeaea2bib99","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3390\/cells12212583","type":"journal-article","article-title":"Correlation of plasma membrane microviscosity and cell stiffness revealed via fluorescence-lifetime imaging and atomic force microscopy","volume":"13","author":"Efremov","year":"2023","journal-title":"Cells"},{"key":"mafaeaea2bib100","doi-asserted-by":"publisher","first-page":"2041","DOI":"10.1021\/jacsau.4c00237","type":"journal-article","article-title":"Visualizing actin packing and the effects of actin attachment on lipid membrane viscosity using molecular rotors","volume":"4","author":"Ioannou","year":"2024","journal-title":"JACS Au."},{"key":"mafaeaea2bib101","doi-asserted-by":"publisher","first-page":"4361","DOI":"10.1021\/acsabm.1c00135","type":"journal-article","article-title":"A tunable palette of molecular rotors allows multicolor, ratiometric fluorescence imaging and direct mapping of mitochondrial heterogeneity","volume":"4","author":"Raja","year":"2021","journal-title":"ACS Appl. Bio Mater."},{"key":"mafaeaea2bib102","doi-asserted-by":"publisher","first-page":"4609","DOI":"10.1002\/slct.201700463","type":"journal-article","article-title":"Facile synthesis of highly sensitive, red-emitting, fluorogenic dye for microviscosity and mitochondrial imaging in embryonic stem cells","volume":"2","author":"Raja","year":"2017","journal-title":"ChemistrySelect"},{"key":"mafaeaea2bib103","doi-asserted-by":"publisher","first-page":"2459","DOI":"10.1039\/d0qm00872a","type":"journal-article","article-title":"Near infrared emitting molecular rotor based on merocyanine for probing the viscosity of cellular lipid environments","volume":"5","author":"Mukherjee","year":"2021","journal-title":"Mater. Chem. Front."},{"key":"mafaeaea2bib104","doi-asserted-by":"publisher","first-page":"13269","DOI":"10.1039\/C6CC05954F","type":"journal-article","article-title":"Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor","volume":"52","author":"Dent","year":"2016","journal-title":"Chem. Commun."},{"key":"mafaeaea2bib105","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1038\/s41589-020-0529-6","type":"journal-article","article-title":"Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape","volume":"16","author":"Lorent","year":"2020","journal-title":"Nat. Chem. Biol."},{"key":"mafaeaea2bib106","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1002\/anie.202113163","type":"journal-article","article-title":"Photocleavable fluorescent membrane tension probes: fast release with spatiotemporal control in inner leaflets of plasma membrane, nuclear envelope, and secretory pathway","author":"L\u00f3pez-Andarias","year":"2022","journal-title":"Angew. Chem., Int. Ed."},{"key":"mafaeaea2bib107","doi-asserted-by":"publisher","first-page":"16768","DOI":"10.1002\/chem.202102743","type":"journal-article","article-title":"Designing a red-emitting viscosity-sensitive bodipy fluorophore for intracellular viscosity imaging","volume":"27","author":"Maleckait\u0117","year":"2021","journal-title":"Chem. Eur. J."},{"key":"mafaeaea2bib108","doi-asserted-by":"publisher","first-page":"2828","DOI":"10.1039\/C5TC02954F","type":"journal-article","article-title":"Tuning BODIPY molecular rotors into the red: sensitivity to viscosity vs. temperature","volume":"4","author":"Vu","year":"2016","journal-title":"J. Mater. Chem. C"},{"key":"mafaeaea2bib109","doi-asserted-by":"publisher","first-page":"2158","DOI":"10.1021\/acssensors.0c02275","type":"journal-article","article-title":"Cyclopropyl substituents transform the viscosity-sensitive BODIPY molecular rotor into a temperature sensor","volume":"6","author":"Vy\u0161niauskas","year":"2021","journal-title":"ACS Sens."},{"key":"mafaeaea2bib110","doi-asserted-by":"publisher","first-page":"7282","DOI":"10.1021\/acs.jpcb.9b04384","type":"journal-article","article-title":"Sensing temperature in vitro and in cells using a BODIPY molecular probe","volume":"123","author":"Ogle","year":"2019","journal-title":"J. Phys. Chem. B"},{"key":"mafaeaea2bib111","doi-asserted-by":"publisher","first-page":"14935","DOI":"10.1002\/chem.201903604","type":"journal-article","article-title":"Fluorescent flipper probes: comprehensive twist coverage","volume":"25","author":"Strakova","year":"2019","journal-title":"Chem. Eur. J."},{"key":"mafaeaea2bib112","doi-asserted-by":"publisher","DOI":"10.1002\/ceur.202300041","type":"journal-article","article-title":"Hydrophobic interfacing of fluorescent membrane probes","volume":"1","author":"Bayard","year":"2023","journal-title":"ChemistryEurope"},{"key":"mafaeaea2bib113","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1021\/ja5107018","type":"journal-article","article-title":"Fluorescent flippers for mechanosensitive membrane probes","volume":"137","author":"Dal Molin","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib114","doi-asserted-by":"publisher","first-page":"3792","DOI":"10.1039\/c4ob02507e","type":"journal-article","article-title":"Thiophene-based dyes for probing membranes","volume":"13","author":"L\u00f3pez-Duarte","year":"2015","journal-title":"Org. Biomol. Chem."},{"key":"mafaeaea2bib115","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1038\/ncomms2273","type":"journal-article","article-title":"Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution","volume":"3","author":"Owen","year":"2012","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib116","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.tcb.2020.01.009","type":"journal-article","article-title":"Lipid rafts: controversies resolved, mysteries remain","volume":"30","author":"Levental","year":"2020","journal-title":"Trends Cell Biol."},{"key":"mafaeaea2bib117","doi-asserted-by":"publisher","first-page":"1979","DOI":"10.1038\/s41596-018-0026-5","type":"journal-article","article-title":"Fit-free analysis of fluorescence lifetime imaging data using the phasor approach","volume":"13","author":"Ranjit","year":"2018","journal-title":"Nat. Protocols"},{"key":"mafaeaea2bib118","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/nprot.2011.419","type":"journal-article","article-title":"Quantitative imaging of membrane lipid order in cells and organisms","volume":"7","author":"Owen","year":"2012","journal-title":"Nat. Protocols"},{"key":"mafaeaea2bib119","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202309721","type":"journal-article","article-title":"Fluorescent solvatochromic probes for long-term imaging of lipid order in living cells","volume":"11","author":"Tanaka","year":"2024","journal-title":"Adv. Sci."},{"key":"mafaeaea2bib120","doi-asserted-by":"publisher","first-page":"14798","DOI":"10.1021\/acs.analchem.0c03559","type":"journal-article","article-title":"Redesigning solvatochromic probe Laurdan for imaging lipid order selectively in cell plasma membranes","volume":"92","author":"Danylchuk","year":"2020","journal-title":"Anal. Chem."},{"key":"mafaeaea2bib121","doi-asserted-by":"publisher","first-page":"14450","DOI":"10.1039\/c6cc08771j","type":"journal-article","article-title":"Headgroup engineering in mechanosensitive membrane probes","volume":"52","author":"Soleimanpour","year":"2016","journal-title":"Chem. Commun."},{"key":"mafaeaea2bib122","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1039\/d4fd00205a","type":"journal-article","article-title":"Immune cell activation produces locally scrambled foci of plasma membrane lipids","volume":"259","author":"Sputay","year":"2025","journal-title":"Faraday Discuss"},{"key":"mafaeaea2bib123","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2417145122","type":"journal-article","article-title":"Loss of lipid asymmetry facilitates plasma membrane blebbing by decreasing membrane lipid packing","volume":"122","author":"Wang","year":"2025","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"mafaeaea2bib124","doi-asserted-by":"publisher","first-page":"4398","DOI":"10.1021\/acsnano.8b00177","type":"journal-article","article-title":"An optical technique for mapping microviscosity dynamics in cellular organelles","volume":"12","author":"Chambers","year":"2018","journal-title":"ACS Nano"},{"key":"mafaeaea2bib125","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1126\/sciadv.abm2094","type":"journal-article","article-title":"Z-\u03b1 1 -antitrypsin polymers impose molecular filtration in the endoplasmic reticulum after undergoing phase transition to a solid state","volume":"8","author":"Chambers","year":"2022","journal-title":"Sci. Adv."},{"key":"mafaeaea2bib126","doi-asserted-by":"publisher","DOI":"10.1088\/2050-6120\/ab481f","type":"journal-article","article-title":"Linker length affects photostability of protein-targeted sensor of cellular microviscosity","volume":"7","author":"Kub\u00e1nkov\u00e1","year":"2019","journal-title":"Methods Appl. Fluoresc."},{"key":"mafaeaea2bib127","doi-asserted-by":"publisher","first-page":"8694","DOI":"10.1039\/c2cc33133k","type":"journal-article","article-title":"Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag","volume":"48","author":"Gatzogiannis","year":"2012","journal-title":"Chem. Commun."},{"key":"mafaeaea2bib128","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1021\/acscentsci.0c00666","type":"journal-article","article-title":"Haloflippers: a general tool for the fluorescence imaging of precisely localized membrane tension changes in living cells","volume":"6","author":"Strakova","year":"2020","journal-title":"ACS Cent. Sci."},{"key":"mafaeaea2bib129","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1039\/C8SC03620A","type":"journal-article","article-title":"Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells","volume":"10","author":"Goujon","year":"2019","journal-title":"Chem. Sci."},{"key":"mafaeaea2bib130","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1021\/jacsau.0c00069","type":"journal-article","article-title":"Genetically encoded supramolecular targeting of fluorescent membrane tension probes within live cells: precisely localized controlled release by external chemical stimulation","volume":"1","author":"L\u00f3pez-Andarias","year":"2021","journal-title":"JACS Au."},{"key":"mafaeaea2bib131","doi-asserted-by":"publisher","first-page":"13210","DOI":"10.1002\/chem.201601925","type":"journal-article","article-title":"A molecular rotor that measures dynamic changes of lipid bilayer viscosity caused by oxidative stress","volume":"22","author":"Vy\u0161niauskas","year":"2016","journal-title":"Chem. Eur. J."},{"key":"mafaeaea2bib132","doi-asserted-by":"publisher","first-page":"9466","DOI":"10.1039\/c8sm01633j","type":"journal-article","article-title":"Molecular rotors report on changes in live cell plasma membrane microviscosity upon interaction with beta-amyloid aggregates","volume":"14","author":"Kub\u00e1nkov\u00e1","year":"2018","journal-title":"Soft Matter"},{"key":"mafaeaea2bib133","doi-asserted-by":"publisher","first-page":"2060","DOI":"10.1016\/j.bbamem.2016.06.003","type":"journal-article","article-title":"Effect of ethanol perturbation on viscosity and permeability of an inner membrane in Bacillus subtilis spores","volume":"1858","author":"Loison","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"mafaeaea2bib134","doi-asserted-by":"publisher","first-page":"36307","DOI":"10.1021\/acsami.9b10426","type":"journal-article","article-title":"Microscopic viscosity of neuronal plasma membranes measured using fluorescent molecular rotors: effects of oxidative stress and neuroprotection","volume":"11","author":"Kub\u00e1nkov\u00e1","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"mafaeaea2bib135","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2021.112346","type":"journal-article","article-title":"Visualising UV-A light-induced damage to plasma membranes of eye lens","volume":"225","author":"Sherin","year":"2021","journal-title":"J. Photochem. Photobiol. B"},{"key":"mafaeaea2bib136","doi-asserted-by":"publisher","DOI":"10.3791\/62708","type":"journal-article","article-title":"Probing metabolism and viscosity of cancer cells using fluorescence lifetime imaging microscopy","volume":"2021","author":"Shimolina","year":"2021","journal-title":"J. Vis. Exp."},{"key":"mafaeaea2bib137","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1088\/2050-6120\/ac89cd","type":"journal-article","article-title":"Development of resistance to 5-fluorouracil affects membrane viscosity and lipid composition of cancer cells","author":"Shimolina","year":"2022","journal-title":"Methods Appl. Fluoresc."},{"key":"mafaeaea2bib138","doi-asserted-by":"publisher","first-page":"1984","DOI":"10.3390\/biomedicines12112550","type":"journal-article","article-title":"Unraveling microviscosity changes induced in cancer cells by photodynamic therapy with targeted genetically encoded photosensitizer","volume":"12","author":"Shimolina","year":"2024","journal-title":"Biomedicines"},{"key":"mafaeaea2bib139","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/nchem.120","type":"journal-article","article-title":"Imaging intracellular viscosity of a single cell during photoinduced cell death","volume":"1","author":"Kuimova","year":"2009","journal-title":"Nat. Chem."},{"key":"mafaeaea2bib140","doi-asserted-by":"publisher","first-page":"1984","DOI":"10.1016\/j.bpj.2019.03.038","type":"journal-article","article-title":"Measuring intracellular viscosity in conditions of hypergravity","volume":"116","author":"Woodcock","year":"2019","journal-title":"Biophys. J."},{"key":"mafaeaea2bib141","doi-asserted-by":"publisher","first-page":"21615","DOI":"10.1002\/ange.202008224","type":"journal-article","article-title":"Simultaneous detection of carbon monoxide and viscosity changes in cells","volume":"132","author":"Robson","year":"2020","journal-title":"Angew. Chem., Int. Ed."},{"key":"mafaeaea2bib142","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70972-5","type":"journal-article","article-title":"Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIM","volume":"10","author":"Kashirina","year":"2020","journal-title":"Sci. Rep."},{"key":"mafaeaea2bib143","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1117\/1.JBO.25.12.126004","type":"journal-article","article-title":"Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy","volume":"25","author":"Shimolina","year":"2020","journal-title":"J. Biomed. Opt."},{"key":"mafaeaea2bib144","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrp.2025.102567","type":"journal-article","article-title":"Elastic remodeling of model and cell membranes by rotating ATP synthase","volume":"6","author":"Valdivieso Gonz\u00e1lez","year":"2025","journal-title":"Cell Rep. Phys. Sci."},{"key":"mafaeaea2bib145","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-65571-9","type":"journal-article","article-title":"Adherent cells sustain membrane tension gradients independently of migration","volume":"16","author":"Garc\u00eda-arcos","year":"2025","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib146","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2024.113007","type":"journal-article","article-title":"Photodynamic therapy with Photoditazine increases microviscosity of cancer cells membrane in cellulo and in vivo","volume":"259","author":"Shimolina","year":"2024","journal-title":"J. Photochem. Photobiol. B"},{"key":"mafaeaea2bib147","doi-asserted-by":"publisher","first-page":"9952","DOI":"10.1021\/acs.langmuir.1c00830","type":"journal-article","article-title":"Lipid hydroperoxide compromises the membrane structure organization and softens bending rigidity","volume":"37","author":"Scanavachi","year":"2021","journal-title":"Langmuir"},{"key":"mafaeaea2bib148","doi-asserted-by":"publisher","first-page":"12752","DOI":"10.1021\/jacs.5b06800","type":"journal-article","article-title":"Curvature softening and negative compressibility of gel-phase lipid membranes","volume":"137","author":"Diggins","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib149","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1038\/s41556-018-0150-z","type":"journal-article","article-title":"Decrease in plasma membrane tension triggers PtdIns(4,5)P 2 phase separation to inactivate TORC2","volume":"20","author":"Riggi","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"mafaeaea2bib150","doi-asserted-by":"publisher","first-page":"5714","DOI":"10.1021\/acsbiomaterials.4c00911","type":"journal-article","article-title":"Influence of simvastatin and pravastatin on the biophysical properties of model lipid bilayers and plasma membranes of live cells","volume":"10","author":"Polita","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"mafaeaea2bib151","doi-asserted-by":"publisher","first-page":"4322","DOI":"10.1021\/acsnano.1c10534","type":"journal-article","article-title":"Substrate stiffness-driven membrane tension modulates vesicular trafficking via Caveolin-1","volume":"16","author":"Lachowski","year":"2022","journal-title":"ACS Nano"},{"key":"mafaeaea2bib152","doi-asserted-by":"publisher","first-page":"6165","DOI":"10.3390\/cancers13246165","type":"journal-article","article-title":"The role of plasma membrane viscosity in the response and resistance of cancer cells to oxaliplatin","volume":"13","author":"Shimolina","year":"2021","journal-title":"Cancers"},{"key":"mafaeaea2bib153","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.jphotobiol.2018.03.002","type":"journal-article","article-title":"Evaluation of membrane fluidity of multidrug-resistant isolates of Escherichia coli and Staphylococcus aureus in presence and absence of antibiotics","volume":"181","author":"Bessa","year":"2018","journal-title":"J. Photochem. Photobiol. B"},{"key":"mafaeaea2bib154","doi-asserted-by":"publisher","first-page":"10251","DOI":"10.1007\/s00253-016-7975-9","type":"journal-article","article-title":"Mechanical properties that influence antimicrobial peptide activity in lipid membranes","volume":"100","author":"Mar\u00edn-Medina","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"mafaeaea2bib155","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2512977122","type":"journal-article","article-title":"Exploring bacteria\u2013surface interactions with a fluorescent membrane tension probe","volume":"122","author":"Gonzalez-Garcia","year":"2025","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"mafaeaea2bib156","doi-asserted-by":"publisher","first-page":"13148","DOI":"10.1021\/acs.langmuir.1c02392","type":"journal-article","article-title":"Peptide-membrane interactions monitored by fluorescence lifetime imaging: a study case of transportan 10","volume":"37","author":"Anselmo","year":"2021","journal-title":"Langmuir"},{"key":"mafaeaea2bib157","doi-asserted-by":"publisher","first-page":"6081","DOI":"10.1038\/s41467-023-41709-5","type":"journal-article","article-title":"Biomolecular condensates modulate membrane lipid packing and hydration","volume":"14","author":"Mangiarotti","year":"2023","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib158","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1039\/d1na00755f","type":"journal-article","article-title":"Addressing challenges in the removal of unbound dye from passively labelled extracellular vesicles","volume":"4","author":"Rautaniemi","year":"2022","journal-title":"Nanoscale Adv."},{"key":"mafaeaea2bib159","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/s41592-018-0291-9","type":"journal-article","article-title":"A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM","volume":"16","author":"Castello","year":"2019","journal-title":"Nat. Methods"},{"key":"mafaeaea2bib160","doi-asserted-by":"publisher","first-page":"12595","DOI":"10.1021\/acs.analchem.5c00826","type":"journal-article","article-title":"Super-resolution FLIM imaging of intracellular lipid microenvironments with a dual-responsive polarity- and pH-sensitive fluorescent probe","volume":"97","author":"Cao","year":"2025","journal-title":"Anal. Chem."},{"key":"mafaeaea2bib161","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1038\/s42003-021-02976-4","type":"journal-article","article-title":"Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells","volume":"5","author":"Oleksiievets","year":"2022","journal-title":"Commun. Biol."},{"key":"mafaeaea2bib162","doi-asserted-by":"publisher","first-page":"7997","DOI":"10.1021\/acs.jpcb.4c02506","type":"journal-article","article-title":"Single-molecule localization microscopy and tracking with a fluorescent mechanosensitive probe","volume":"128","author":"Maillard","year":"2024","journal-title":"J. Phys. Chem. B"},{"key":"mafaeaea2bib163","doi-asserted-by":"publisher","first-page":"12034","DOI":"10.1021\/jacs.0c04942","type":"journal-article","article-title":"Fluorescent membrane tension probes for super-resolution microscopy: combining mechanosensitive cascade switching with dynamic-covalent ketone chemistry","volume":"142","author":"Garc\u00eda-Calvo","year":"2020","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib164","doi-asserted-by":"publisher","first-page":"22609","DOI":"10.1021\/jacs.5c03749","type":"journal-article","article-title":"Deep learning for fluorescence lifetime predictions enables high-throughput in vivo imaging","volume":"147","author":"Kapsiani","year":"2025","journal-title":"J. Am. Chem. Soc."},{"key":"mafaeaea2bib165","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1002\/cyto.a.22616","type":"journal-article","article-title":"Time-domain microfluidic fluorescence lifetime flow cytometry for high-throughput F\u00f6rster resonance energy transfer screening","volume":"87","author":"Nedbal","year":"2015","journal-title":"Cytometry A"},{"key":"mafaeaea2bib166","doi-asserted-by":"publisher","first-page":"7376","DOI":"10.1038\/s41467-024-51125-y","type":"journal-article","article-title":"High-throughput fluorescence lifetime imaging flow cytometry","volume":"15","author":"Kanno","year":"2024","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib167","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1038\/s41467-017-01325-6","type":"journal-article","article-title":"Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish","volume":"8","author":"Lagendijk","year":"2017","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib168","doi-asserted-by":"publisher","first-page":"1674","DOI":"10.1038\/s41565-024-01723-0","type":"journal-article","article-title":"Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors","volume":"19","author":"Hu","year":"2024","journal-title":"Nat. Nanotechnol."},{"key":"mafaeaea2bib169","doi-asserted-by":"publisher","first-page":"2510","DOI":"10.1038\/s41467-021-22606-1","type":"journal-article","article-title":"Super-resolved visualization of single DNA-based tension sensors in cell adhesion","volume":"12","author":"Schlichthaerle","year":"2021","journal-title":"Nat. Commun."},{"key":"mafaeaea2bib170","doi-asserted-by":"publisher","first-page":"7579","DOI":"10.1021\/acs.nanolett.2c02605","type":"journal-article","article-title":"Advanced DNA Zipper probes for detecting cell membrane lipid domains","volume":"22","author":"Ali","year":"2022","journal-title":"Nano Lett."},{"key":"mafaeaea2bib171","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202112033","type":"journal-article","article-title":"Imaging membrane order and dynamic interactions in living cells with a DNA zipper probe","volume":"61","author":"Bagheri","year":"2022","journal-title":"Angew. Chem., Int. Ed."}],"container-title":["Methods and Applications in Fluorescence"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2","content-type":"text\/html","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2\/pdf","content-type":"application\/pdf","content-version":"am","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:01:53Z","timestamp":1791810113000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1088\/2050-6120\/aeaea2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,12]]},"references-count":171,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,10,12]]},"published-print":{"date-parts":[[2026,12,1]]}},"URL":"https:\/\/doi.org\/10.1088\/2050-6120\/aeaea2","relation":{},"ISSN":["2050-6120"],"issn-type":[{"value":"2050-6120","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,12]]},"assertion":[{"value":"Recent developments in fluorescence lifetime imaging of lipid membrane dynamics","name":"article_title","label":"Article Title"},{"value":"Methods and Applications in Fluorescence","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"\u00a9 2026 The Author(s). Published by IOP Publishing Ltd","name":"copyright_information","label":"Copyright Information"},{"value":"2026-05-19","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-09-30","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2026-10-12","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}