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To illustrate the feasibility of this approach, viable animal tissues were used, including ectocinerea (grey matter), alba (white matter) and blood vessels. Raman spectra were acquired using a custom-built Raman spectrometer. The hyperparameters of the RF model were determined by combining a cross-validation-based algorithm and manually adjusting. The experimental results show the ability of our approach to discriminate different types of tissues with high accuracy.<\/jats:p>","DOI":"10.1088\/2632-2153\/ad1349","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T17:26:51Z","timestamp":1701970011000},"page":"045053","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Using random forest for brain tissue identification by Raman spectroscopy"],"prefix":"10.1088","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3724-3893","authenticated-orcid":true,"given":"Weiyi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chau Minh","family":"Giang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingan","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behnam","family":"Badie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"266","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"mlstad1349bib1","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/j.fsigen.2010.12.001","article-title":"DNA methylation-based forensic tissue identification","volume":"5","author":"Frumkin","year":"2011","journal-title":"Forensic Sci. 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