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In the device, neurites from primary rat dorsal root ganglion (DRG) neurons were capable of forming aligned dense networks in a specific compartment that was physically isolated from neuronal somas. Co-culture of rat DRG neurons and oligodendrocytes, a well-known model to study myelination <jats:italic>in vitro<\/jats:italic>, led to interactions between oligodendrocytes and neurites in the device, and the deposition of myelin segments in an aligned distribution was spontaneously formed. For the fabrication of the device, we present a new method that produces polydimethylsiloxane (PDMS)\u2014based devices possessing an open compartment design. The proposed fabrication method takes advantage of an SU-8 photolithography process and 3D printing for mould fabrication. Both the microscale and macroscale features are replicated from the same mould, allowing devices to be produced with high precision and repeatability. The proposed device is applicable for long-term cell culturing, live-cell imaging, and by enhancing aligned myelin distribution, it is a promising tool for experimental setups that address diverse biological questions in the field of myelin research.<\/jats:p>","DOI":"10.1088\/1361-6439\/ab16a7","type":"journal-article","created":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T23:00:23Z","timestamp":1554505223000},"page":"065009","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":35,"title":["A compartmentalized neuron-oligodendrocyte co-culture device for myelin research: design, fabrication and functionality testing"],"prefix":"10.1088","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3748-3304","authenticated-orcid":false,"given":"Mervi","family":"Ristola","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9181-7355","authenticated-orcid":false,"given":"Lassi","family":"Sukki","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7316-8283","authenticated-orcid":false,"given":"Maria Manuela","family":"Azevedo","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3237-777X","authenticated-orcid":false,"given":"Ana Isabel","family":"Seixas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7636-0924","authenticated-orcid":false,"given":"Jo\u00e3o Bettencourt","family":"Relvas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7602-4418","authenticated-orcid":false,"given":"Susanna","family":"Narkilahti","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2890-6782","authenticated-orcid":false,"given":"Pasi","family":"Kallio","sequence":"additional","affiliation":[]}],"member":"266","published-online":{"date-parts":[[2019,5,3]]},"reference":[{"key":"jmmab16a7bib001","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1146\/annurev-cellbio-100913-013101","volume":"30","author":"Nave","year":"2014","journal-title":"Annu. 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