{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T17:43:05Z","timestamp":1776706985338,"version":"3.51.2"},"reference-count":41,"publisher":"IOP Publishing","issue":"6","license":[{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/page\/copyright"},{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"crossref","award":["ONCOSCREEN project (101097036)"],"award-info":[{"award-number":["ONCOSCREEN project (101097036)"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["CEEC Individual 2021.00049.CEECIND"],"award-info":[{"award-number":["CEEC Individual 2021.00049.CEECIND"]}]},{"name":"Northern Portugal Regional Coordination and Development Commission","award":["PINFRA\/22190\/2016 (Norte-01-0145-FEDER-022190)"],"award-info":[{"award-number":["PINFRA\/22190\/2016 (Norte-01-0145-FEDER-022190)"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biomed. Mater."],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Integrating biological material within soft microfluidic systems made of hydrogels offers countless possibilities in biomedical research to overcome the intrinsic limitations of traditional microfluidics based on solid, non-biodegradable, and non-biocompatible materials. Hydrogel-based microfluidic technologies have the potential to transform <jats:italic>in vitro<\/jats:italic> cell\/tissue culture and modeling. However, most hydrogel-based microfluidic platforms are associated with device deformation, poor structural definition, reduced stability\/reproducibility due to swelling, and a limited range in rigidity, which threatens their applicability. Herein, we describe a new methodological approach for developing a soft cell-laden microfluidic device based on enzymatically-crosslinked silk fibroin (SF) hydrogels. Its unique mechano-chemical properties and high structural fidelity, make this platform especially suited for <jats:italic>in vitro<\/jats:italic> disease modelling, as demonstrated by reproducing the native dynamic 3D microenvironment of colorectal cancer and its response to chemotherapeutics in a simplistic way. Results show that from all the tested concentrations, 14 wt% enzymatically-crosslinked SF microfluidic platform has outstanding structural stability and the ability to perfuse fluid while displaying <jats:italic>in vivo<\/jats:italic>-like biological responses. Overall, this work shows a novel technique to obtain an enzymatically-crosslinked SF microfluidic platform that can be employed for developing soft lab-on-a-chip <jats:italic>in vitro<\/jats:italic> models.<\/jats:p>","DOI":"10.1088\/1748-605x\/ad8829","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T22:54:56Z","timestamp":1729205696000},"page":"065032","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Biomimetic and soft lab-on-a-chip platform based on enzymatic-crosslinked silk fibroin hydrogel for 3D cell co-culture"],"prefix":"10.1088","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2081-3909","authenticated-orcid":true,"given":"Mariana R","family":"Carvalho","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7930-2535","authenticated-orcid":true,"given":"David","family":"Caballero","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7170-2291","authenticated-orcid":true,"given":"Subhas C","family":"Kundu","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4295-6129","authenticated-orcid":false,"given":"Rui L","family":"Reis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7052-8837","authenticated-orcid":true,"given":"Joaquim M","family":"Oliveira","sequence":"additional","affiliation":[]}],"member":"266","published-online":{"date-parts":[[2024,10,24]]},"reference":[{"key":"bmmad8829bib1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biosx.2022.100106","article-title":"Emergence of microfluidics for next generation biomedical devices","volume":"10","author":"Preetam","year":"2022","journal-title":"Biosens. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2024-01-09","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2024-10-17","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2024-10-24","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}