{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T21:08:27Z","timestamp":1775682507257,"version":"3.50.1"},"reference-count":51,"publisher":"IOP Publishing","issue":"4","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/publishingsupport.iopscience.iop.org\/iop-standard\/v1"},{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["CEECIND\/01375\/2017"],"award-info":[{"award-number":["CEECIND\/01375\/2017"]}]}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Biofabrication"],"published-print":{"date-parts":[[2021,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Decellularized extracellular matrices (ECMs) are able to provide the necessary and specific cues for remodeling and maturation of tissue-specific cells. Nevertheless, their use for typical biofabrication applications requires chemical modification or mixing with other polymers, mainly due to the limited viscoelastic properties. In this study, we hypothesize that a bioink exclusively based on decellularized kidney ECM (dKECM) could be used to bioprint renal progenitor cells. To address these aims, porcine kidneys were decellularized, lyophilized and digested to yield a viscous solution. Then, the bioprinting process was optimized using an agarose microparticle support bath containing transglutaminase for enzymatic crosslinking of the dKECM. This methodology was highly effective to obtain constructs with good printing resolution and high structural integrity. Moreover, the encapsulation of primary renal progenitor cells resulted in high cell viability, with creation of 3D complex structures over time. More importantly, this tissue-specific matrix was also able to influence cellular growth and differentiation over time. Taken together, these results demonstrate that unmodified dKECM bioinks have great potential for bioengineering renal tissue analogs with promising translational applications and\/or for\n                    <jats:italic>in vitro<\/jats:italic>\n                    model systems. Ultimately, this strategy may have greater implications on the biomedical field for the development of bioengineered substitutes using decellularized matrices from other tissues.\n                  <\/jats:p>","DOI":"10.1088\/1758-5090\/ac0fca","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T18:45:30Z","timestamp":1624992330000},"page":"045006","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":59,"title":["Decellularized kidney extracellular matrix bioinks recapitulate renal 3D microenvironment\n                    <i>in vitro<\/i>"],"prefix":"10.1088","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4433-8890","authenticated-orcid":false,"given":"Rita","family":"Sobreiro-Almeida","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7758-1251","authenticated-orcid":false,"given":"Manuel","family":"G\u00f3mez-Florit","sequence":"additional","affiliation":[]},{"given":"Rita","family":"Quinteira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4295-6129","authenticated-orcid":false,"given":"Rui L","family":"Reis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2036-6291","authenticated-orcid":false,"given":"Manuela E","family":"Gomes","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3041-0687","authenticated-orcid":false,"given":"Nuno M","family":"Neves","sequence":"additional","affiliation":[]}],"member":"266","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"bfac0fcabib1","doi-asserted-by":"publisher","first-page":"1975","DOI":"10.1016\/S0140-6736(14)61601-9","type":"journal-article","article-title":"Worldwide access to treatment for end-stage kidney disease: a systematic review","volume":"385","author":"Liyanage","year":"2015","journal-title":"Lancet"},{"key":"bfac0fcabib2","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1016\/S0140-6736(16)31012-1","type":"journal-article","article-title":"Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: a systematic analysis for the global burden of disease study 2015","volume":"388","author":"Wang","year":"2016","journal-title":"Lancet"},{"key":"bfac0fcabib3","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.1039\/c8bm00073e","type":"journal-article","article-title":"Development of non-orthogonal 3D-printed scaffolds to enhance their osteogenic performance","volume":"6","author":"Fonseca","year":"2018","journal-title":"Biomater. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2020-10-30","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2021-06-29","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2021-07-26","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}