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Phys. Eng. Express"],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pulsed laser deposition (PLD) represents a promising bottom-up methodology for the synthesis and transference of nanoparticles to the surface of a biomedical device. Silver (Ag) nanoparticles directly incorporated on the metallic implant emerge as an alternative strategy for local action against prosthetic joint-associated infections. In the present research, a dual sequential PLD process is proposed to obtain a bilayer coating with (1) a bio-derived calcium phosphate (CaP) layer, to provide osteointegrative properties and (2) the controlled growth of the Ag nanoparticles over it, ranging the number of laser pulses from 100 to 500. The characterization by SEM, EDS, TEM, XPS and AFM revealed the uniform deposit of Ag rounded nanoparticles, with a narrow mean size distribution, in the original non-oxidized metallic state. Moreover, given the evidences from XPS and AFM techniques, the occurrence of a coalescence phenomenon from 400 pulses onwards was proposed together with the expected positive linear relation between the number of pulses and Ag contribution with a deposition rate of 0.05 at. % of Ag per pulse. Conversely, the decrease in roughness as the Ag content increased was also verified. Finally, the expected bacteriostatic activity for these PLD deposited metallic state Ag nanoparticles against the bacterial strain\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    was confirmed. Moreover, the evaluation of the osteoblast-like MG-63 cells viability on the Ag(100\u2013500)-CaP coatings revealed a significant increased proliferation (p &gt; 0.05) on the Ag100-CaP coating compared to the control (Ag0-CaP). When same coating was evaluated against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    the effect was not significant. The possibility of modulating the amount of nanoparticles in the bilayer coating to obtain a greater or lesser effect in combination with CaP was revealed.\n                  <\/jats:p>","DOI":"10.1088\/2057-1976\/ac9846","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T18:11:47Z","timestamp":1665166307000},"page":"065019","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Dual pulsed laser deposition of Ag nanoparticles on calcium phosphate coatings for biomedical applications"],"prefix":"10.1088","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0118-7412","authenticated-orcid":true,"given":"M","family":"L\u00f3pez-\u00c1lvarez","sequence":"first","affiliation":[]},{"given":"L","family":"Gonz\u00e1lez-Rodr\u00edguez","sequence":"additional","affiliation":[]},{"given":"F","family":"Gontad","sequence":"additional","affiliation":[]},{"given":"R","family":"Teixeira-Santos","sequence":"additional","affiliation":[]},{"given":"M","family":"Doiro","sequence":"additional","affiliation":[]},{"given":"L","family":"\u00c1lvarez-G\u00f3mez","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5233-1037","authenticated-orcid":true,"given":"F J M","family":"Mergulh\u00e3o","sequence":"additional","affiliation":[]},{"given":"P","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[]},{"given":"J","family":"Serra","sequence":"additional","affiliation":[]}],"member":"266","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"bpexac9846bib1","doi-asserted-by":"publisher","first-page":"145299","DOI":"10.1016\/j.apsusc.2020.145299","type":"journal-article","article-title":"Manipulation of AgNPs coated on selenium\/carbonated hydroxyapatite\/\u03b5-polycaprolactone nano-fibrous via pulsed laser deposition for wound healing applications","volume":"508","author":"Menazea","year":"2020","journal-title":"Appl. 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All rights, including for text and data mining, AI training, and similar technologies, are reserved.","name":"copyright_information","label":"Copyright Information"},{"value":"2022-05-25","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2022-10-07","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2022-10-18","name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}