{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T21:55:20Z","timestamp":1770760520212,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2001,6,1]],"date-time":"2001-06-01T00:00:00Z","timestamp":991353600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2001,6]]},"abstract":"<jats:p>\n                    To determine and type the extended-spectrum \u03b2-lactamases (ESBLs) among the family Enterobacteriaceae in a medical center, a total of 668 clinical isolates were screened. Of the 668 isolates, the 80\n         strains were presumptively defined as ESBL producers according to the result of disk method using ESBL marker antibiotics (aztreonam, ceftazidime, and cefoxitin). These 80 strains were retested with the\n         double-disk synergy test (DDST), the E-test ESBL strip, a 5-\u00b5g ceftazidime disk, and agar dilution MICs of ceftazidime with and without clavulonic acid. Isoelectric focusing was performed to confirm\n         ESBL production and type the \u03b2-lactamases. By evaluation of the results of all tests used for ESBL detection together with isoelectric focusing, 33 (4.9%) of the 668 isolates were described as ESBL\n         producer. The positive results of the agar dilution test, DDST, the E-test strip, and 5-\u00b5g ceftazidime disk were 32, 26, 27, and 26 of the 33 strains, respectively. ESBL positivity was 48.8% in\n                    <jats:italic toggle=\"yes\">Klebsiella<\/jats:italic>\n                    species, 15.4% in\n                    <jats:italic toggle=\"yes\">Citrobacter<\/jats:italic>\n                    species, 4.9% in\n                    <jats:italic toggle=\"yes\">Enterobacter<\/jats:italic>\n                    species and 1.1% in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    strains. The ESBL enzymes frequently determined were SHV-2\/6-like (pI 7.6), SHV-5-like\n         (pI 8.2), SHV-4-like (pI 7.8), and SHV-3-like (pI 7). SHV-derived enzymes were commonly observed in\n                    <jats:italic toggle=\"yes\">Klebsiella<\/jats:italic>\n                    spp whereas TEM-related enzymes were seen in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    strains. The results of\n         this study indicated that SHV-2\/6-derived (pI 7.6) ESBL expression among the isolates of the family Enterobacteriaceae is an important problem in our medical center.\n                  <\/jats:p>","DOI":"10.1089\/10766290152045048","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:05:44Z","timestamp":1027706744000},"page":"171-175","source":"Crossref","is-referenced-by-count":5,"title":["Detection and Typing of Extended-Spectrum \u03b2-Lactamases in Clinical Isolates of the Family Enterobacteriaceae in a Medical Center in Turkey"],"prefix":"10.1177","volume":"7","author":[{"given":"Riza","family":"Durmaz","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Faculty of Medicine, Inonu University, 44069 Malatya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengul","family":"Durmaz","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Faculty of Medicine, Inonu University, 44069 Malatya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet","family":"Koroglu","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Faculty of Medicine, Inonu University, 44069 Malatya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet Sait","family":"Tekerekoglu","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Faculty of Medicine, Inonu University, 44069 Malatya, Turkey."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,6]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290152045048","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290152045048","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:13:54Z","timestamp":1770747234000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/10766290152045048"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,6]]}},"alternative-id":["10.1089\/10766290152045048"],"URL":"https:\/\/doi.org\/10.1089\/10766290152045048","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,6]]}}}