{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T22:20:39Z","timestamp":1770762039335,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:p>\n                    Recently, a nation-wide molecular epidemiologic survey of penicillin-nonsusceptible\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                    has been performed in the Netherlands. In the current study, we analyzed the genes\n                    <jats:italic toggle=\"yes\">pbp1a<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">pbp2b<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">pbp2x<\/jats:italic>\n                    from these clinical isolates at the molecular level, and identified the genetic composition of the penicillin-binding domains. The pneumococcal strains were selected on the\n         basis of differences in restriction fragment length polymorphism (RFLP) patterns of the genes\n                    <jats:italic toggle=\"yes\">pbp1a<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">pbp2b<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">pbp2x<\/jats:italic>\n                    , and represented 8, 7, and 10 distinct patterns, respectively.\n         The genetic heterogeneity observed by sequence analysis of the\n                    <jats:italic toggle=\"yes\">pbp<\/jats:italic>\n                    gene parts was comparable with the heterogeneity of the entire\n                    <jats:italic toggle=\"yes\">pbp<\/jats:italic>\n                    genes as deduced from RFLP analysis. Furthermore, the\n         mutations in the\n                    <jats:italic toggle=\"yes\">pbp<\/jats:italic>\n                    sequences of the Dutch isolates invariably matched with the mutations described in\n                    <jats:italic toggle=\"yes\">pbp<\/jats:italic>\n                    sequences of penicillin-nonsusceptible pneumococci isolated in other countries.\n         Finally, novel mosaic structures were identified indicating horizontal exchange of\n                    <jats:italic toggle=\"yes\">pbp<\/jats:italic>\n                    gene parts among penicillin-nonsusceptible pneumococci.\n                  <\/jats:p>","DOI":"10.1089\/10766290152773338","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:08:35Z","timestamp":1027706915000},"page":"323-334","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Characteristics of Penicillin-Binding Protein Genes of Penicillin-Nonsusceptible\n                    <i>Streptococcus pneumoniae<\/i>\n                    Isolated in The Netherlands"],"prefix":"10.1177","volume":"7","author":[{"given":"Karin","family":"Overweg","sequence":"first","affiliation":[{"name":"Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debby","family":"Bogaert","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Sophia Children's Hospital\/Erasmus University Rotterdam, Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Sluijter","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Sophia Children's Hospital\/Erasmus University Rotterdam, Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"de Groot","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Sophia Children's Hospital\/Erasmus University Rotterdam, Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter W.M.","family":"Hermans","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Sophia Children's Hospital\/Erasmus University Rotterdam, Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290152773338","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/10766290152773338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:13:58Z","timestamp":1770747238000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/10766290152773338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1089\/10766290152773338"],"URL":"https:\/\/doi.org\/10.1089\/10766290152773338","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12]]}}}